Modulation of endoplasmic reticulum stress and cardiomyocyte apoptosis by mulberry leaf diet in experimental autoimmune myocarditis rats

Abstract

Mulberry is commonly used as silkworm diet and an alternative medicine in Japan and China, has recently reported to contain many antioxidative flavanoid compounds and having the free radical scavenging effects. Antioxidants reduce cardiac oxidative stress and attenuate cardiac dysfunction in animals with pacing-induced congestive heart failure. Hence we investigated the cardioprotective effect of mulberry leaf powder in rats with experimental autoimmune myocarditis. Eight-week-old Lewis rats immunized with cardiac myosin were fed with either normal chow or a diet containing 5% mulberry leaf powder and were examined on day 21. ML significantly decreased oxidative stress, myocyte apoptosis, cellular infiltration, cardiac fibrosis, mast cell density, myocardial levels of sarco/endo-plasmic reticulum Ca2+ ATPase2, p22phox, receptor for advanced glycation end products, phospho-p38 mitogen activated protein kinase, phospho-c-Jun NH2-terminal protein kinase, glucose regulated protein78, caspase12 and osteopontin levels in EAM rats. These results may suggest that mulberry diet can preserve the cardiac function in experimental autoimmune myocarditis by modulating oxidative stress induced MAPK activation and further afford protection against endoplasmic reticulum stress mediated apoptosis.

Mulberry leaves and their potential effects against cardiometabolic risks: a review of chemical compositions, biological properties and clinical efficacy

“…In this study, the treatment also preserved cardiac hemodynamic function by reversing systolic and diastolic impairment of myocardium, indicating protective effect on the left ventricular remodelling development (Arumugam et al. 2012 ).…”

“…Similarly, mulberry leaves treatment was associated with the close-to-normal structure of myocardial tissues without much infiltration of inflammatory cytokines and fibrous tissues in the myosin-induced myocarditis models (Arumugam et al. 2012 ). In this study, the treatment also preserved cardiac hemodynamic function by reversing systolic and diastolic impairment of myocardium, indicating protective effect on the left ventricular remodelling development (Arumugam et al.…”

Toki-shakuyaku-san, a Japanese kampo medicine, reduces colon inflammation in a mouse model of acute colitis

“…For the histological analysis, the distal colon samples were fixed immediately in 10% formaldehyde solution, embedded in paraffin, cut into 5 μm thick transversal sections, mounted on glass slides, deparaffinized and stained with hematoxylin and eosin stain (H&E). The slides were monitored under light microscope to check the colonic damage [26].…”

Modified SJH alleviates FFAs-induced hepatic steatosis through leptin signaling pathways

“…Based on the above information, it is conceivable that because of its appreciable antioxidant activities, mSJH can combat fatty acids-induced ER stress in cells. Such an event has also been reflected in a study reporting that the extract of Morus alba leaf, the major constituent of SJH, attenuates ER stress and this effect is principally driven by its antioxidant activity40.…”