Modulation of Epidermal Growth Factor Release by Biopolymer-Coated Liposomes

Parchen, Gabriela Pereira; Jacumazo, Joslaine; Koop, Heidegrid Siebert; Biscaia, Stellee Marcela Petris; Trindade, Edvaldo da Silva; Silveira, Joana Léa Meira; Freitas, Rilton Alves de

doi:10.1016/j.xphs.2020.04.004

Cited by 14 publications

(10 citation statements)

References 48 publications

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“…They found that the amount of bovine serum albumin (BSA) may be significantly enhanced by the increase of the number of deposited bilayers due to the embedding of additional protein molecules within the polyelectrolyte layers. This agrees with the results by Pereira-Parchen et al [252] for the encapsulation of epidermal growth factor, with a 3-fold increase of the encapsulation efficiency for decorated liposomes in relation to the bare one.…”

Section: Lbl Multilayers On Liposomessupporting

confidence: 92%

“…However, the increase of liposome stability depends on both the number of deposited polyelectrolyte layers and the charge density of the liposomal surface, which indicates the important role of the electrostatic interactions as driving force for the stabilization [262]. This is compatible with the finding by Pereira-Parchen et al [252] for the release of epidermal growth factor from liposomes decorated with (CHI-ALG) n multilayers. They reported that the drug release is completely hindered for physiological values of the pH (around 7.2) due to the compact character of the CHI layers, whereas the swelling of the multilayer with the decrease of the pH favors the release of the encapsulated drug.…”

Section: Lbl Multilayers On Liposomessupporting

confidence: 89%

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Polyelectrolyte Multilayers on Soft Colloidal Nanosurfaces: A New Life for the Layer-By-Layer Method

et al. 2021

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The Layer-by-Layer (LbL) method is a well-established method for the assembly of nanomaterials with controlled structure and functionality through the alternate deposition onto a template of two mutual interacting molecules, e.g., polyelectrolytes bearing opposite charge. The current development of this methodology has allowed the fabrication of a broad range of systems by assembling different types of molecules onto substrates with different chemical nature, size, or shape, resulting in numerous applications for LbL systems. In particular, the use of soft colloidal nanosurfaces, including nanogels, vesicles, liposomes, micelles, and emulsion droplets as a template for the assembly of LbL materials has undergone a significant growth in recent years due to their potential impact on the design of platforms for the encapsulation and controlled release of active molecules. This review proposes an analysis of some of the current trends on the fabrication of LbL materials using soft colloidal nanosurfaces, including liposomes, emulsion droplets, or even cells, as templates. Furthermore, some fundamental aspects related to deposition methodologies commonly used for fabricating LbL materials on colloidal templates together with the most fundamental physicochemical aspects involved in the assembly of LbL materials will also be discussed.

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Section: Lbl Multilayers On Liposomessupporting

confidence: 92%

Section: Lbl Multilayers On Liposomessupporting

confidence: 89%

Polyelectrolyte Multilayers on Soft Colloidal Nanosurfaces: A New Life for the Layer-By-Layer Method

et al. 2021

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“…Their pH responsiveness can be used to modulate the release of proteins while protecting them against degradation [ 46 , 47 , 48 , 49 ]. The anionic materials commonly used to form complexes with chitosan include alginate [ 50 , 51 ], collagen [ 52 , 53 ], gelatin [ 9 , 54 , 55 , 56 ], poly(γ-glutamic acid) [ 57 ], β-glycerophosphate [ 58 , 59 , 60 ], and tripolyphosphate [ 47 , 61 ]. Chitosan can associate with synthetic polymers (poly(vinyl alcohol) [ 56 , 62 ], polyethylene glycol [ 63 ], and poly(lactic- co -glycolic acid) [ 64 ]) and other materials (including clays [ 65 ] and graphene oxide [ 52 ]) for producing DDSs with enhanced mechanical properties and hydrophilic–hydrophobic balance.…”

Section: Principal Polysaccharides Used For Biomedical Materialsmentioning

confidence: 99%

Polysaccharide-Based Materials Created by Physical Processes: From Preparation to Biomedical Applications

et al. 2021

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Polysaccharide-based materials created by physical processes have received considerable attention for biomedical applications. These structures are often made by associating charged polyelectrolytes in aqueous solutions, avoiding toxic chemistries (crosslinking agents). We review the principal polysaccharides (glycosaminoglycans, marine polysaccharides, and derivatives) containing ionizable groups in their structures and cellulose (neutral polysaccharide). Physical materials with high stability in aqueous media can be developed depending on the selected strategy. We review strategies, including coacervation, ionotropic gelation, electrospinning, layer-by-layer coating, gelation of polymer blends, solvent evaporation, and freezing–thawing methods, that create polysaccharide-based assemblies via in situ (one-step) methods for biomedical applications. We focus on materials used for growth factor (GFs) delivery, scaffolds, antimicrobial coatings, and wound dressings.

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“…It has been reported that liposome-coated hEGF was more effective at healing wounds than hEGF itself [ 3 ]. Increased stability and localized delivery of hEGF in the injured area has also been reported to be better with liposome coating [ 10 , 11 , 12 , 13 , 14 , 15 ]. Due to its low stability, hEGF with immediate release will also only last for a short time.…”

Section: Introductionmentioning

confidence: 99%

Film-Forming Spray of Water-Soluble Chitosan Containing Liposome-Coated Human Epidermal Growth Factor for Wound Healing

et al. 2021

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Human epidermal growth factor (hEGF) has been known to have excellent wound-healing activity. However, direct application to the wound area can lead to low hEGF bioavailability due to protease enzymes or endocytosis. The use of liposomes as coatings and carriers can protect hEGF from degradation by enzymes, chemical reactions, and immune reactions. Sustained release using a matrix polymer can also keep the levels of hEGF in line with the treatment. Therefore, this study aimed to develop a film-forming spray of water-soluble chitosan (FFSWSC) containing hEGF-liposomes as a potential wound dressing. The hEGF-liposomes were prepared using the hydration film method, and the preparation of the FFSWSC was achieved by the ionic gelation method. The hydration film method produced hEGF-liposomes that were round and spread with a Z-average of 219.3 nm and encapsulation efficiency of 99.87%, whereas the film-forming solution, which provided good sprayability, had a formula containing 2% WSC and 3% propylene glycol with a viscosity, spray angle, droplet size, spray weight, and occlusion factor of 21.94 ± 0.05 mPa.s, 73.03 ± 1.28°, 54.25 ± 13.33 µm, 0.14 ± 0.00 g, and 14.57 ± 3.41%, respectively. The pH, viscosity, and particle size of the FFSWSC containing hEGF-liposomes were stable during storage for a month in a climatic chamber (40 ± 2 °C, RH 75 ± 5%). A wound healing activity test on mice revealed that hEGF-liposomes in FFSWSC accelerated wound closure significantly, with a complete wound closure on day 6. Based on the findings, we concluded that FFSWSC containing hEGF-liposomes has the potential to be used as a wound dressing.

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Modulation of Epidermal Growth Factor Release by Biopolymer-Coated Liposomes

Cited by 14 publications

References 48 publications

Polyelectrolyte Multilayers on Soft Colloidal Nanosurfaces: A New Life for the Layer-By-Layer Method

Polyelectrolyte Multilayers on Soft Colloidal Nanosurfaces: A New Life for the Layer-By-Layer Method

Polysaccharide-Based Materials Created by Physical Processes: From Preparation to Biomedical Applications

Film-Forming Spray of Water-Soluble Chitosan Containing Liposome-Coated Human Epidermal Growth Factor for Wound Healing

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