2017
DOI: 10.4236/jbise.2017.102005
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Modulation of Fatty Acid Oxidation and Glucose Uptake by Oxytocin in Adipocytes

Abstract: Oxytocin (OT) is a hypothalamic neuropeptide synthesized and secreted by OT neurons. In addition to its conventional role in reproductive physiology, central OT also regulates various social behaviors, such as care, trust, and emotions. Central and subcutaneous OT infusions stimulate lipid metabolism in mice and rats when fed standard or high fat diets. Mice lacking the OT receptor (OTR) or OT peptide develop late-onset obesity with greater fat pad weights, larger adipocyte size and elevated plasma levels of l… Show more

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“…The results in Fig 2L represented 8 repeats for EEC culture experiment. After homogenization procedure and preliminary centrifugation (17K x g at 4°C for 15 minutes) to remove mitochondria, cell membranes, nucleus and intact cells, the supernatant from EECs were sedimented at 145K x g for 2 hours (CP80WX, Hitachi Koki Co., Ltd., Hitachi, Tokyo, Japan) to collect microsomes, then were sonicated and stored in -20°C for Western blotting following the previously described procedure [ 19 ]. In brief, microsomes (10 μg protein/sample as determined by the method of Bradford reagent (B6916, Sigma-Aldrich, according to the manufacturer’s protocol)) were subjected to 10% SDS-PAGE gel, and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membrane (NEF1002001PK, PerkinElmer, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…The results in Fig 2L represented 8 repeats for EEC culture experiment. After homogenization procedure and preliminary centrifugation (17K x g at 4°C for 15 minutes) to remove mitochondria, cell membranes, nucleus and intact cells, the supernatant from EECs were sedimented at 145K x g for 2 hours (CP80WX, Hitachi Koki Co., Ltd., Hitachi, Tokyo, Japan) to collect microsomes, then were sonicated and stored in -20°C for Western blotting following the previously described procedure [ 19 ]. In brief, microsomes (10 μg protein/sample as determined by the method of Bradford reagent (B6916, Sigma-Aldrich, according to the manufacturer’s protocol)) were subjected to 10% SDS-PAGE gel, and the separated proteins were electrophoretically transferred to a polyvinylidene difluoride (PVDF) membrane (NEF1002001PK, PerkinElmer, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%