Modulation of gastrointestinal digestion of β-lactoglobulin and micellar casein following binding by (−)-epigallocatechin-3-gallate (EGCG) and green tea flavanols

Dönmez, Özge; Mogol, Burçe Ataç; Gökmen, Vural; Tang, Ning; Andersen, Mogens L.; Chatterton, Dereck E.W.

doi:10.1039/d0fo00783h

Cited by 18 publications

(9 citation statements)

References 52 publications

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“…In addition, some studies have focused on gastrointestinal digestion of protein-polyphenols conjugates and their emulsion system, although these studies did not show any newly formed toxicants of conjugation of protein and polyphenols as well as their consumption. 23,46,47 The results of the present study indicate that the conjugation of native-pcRPI with EGCG significantly increases the antioxidant capacity of native-pcRPI.…”

Section: Secondary Structure Of Pcrpi-egcgsupporting

confidence: 49%

Conjugation of (–)‐epigallocatechin‐3‐gallate and protein isolate from large yellow croaker (Pseudosciaena crocea) roe: improvement of antioxidant activity and structural characteristics

Han

Yin

et al. 2021

J Sci Food Agric

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BACKGROUND: Large yellow croaker (Pseudosciaena crocea) roe is the main by-product in the processing of large yellow croaker. Previous studies have found that its protein isolates are composed of vitellogenin, as well as vitellogenin B and C, having good functional properties. (-)-Epigallocatechin-3-gallate (EGCG) is a natural antioxidant component that can be combined with protein to improve antioxidant activity and structural characteristics of protein.RESULTS: EGCG was bound with the P. crocea roe protein isolate (pcRPI) by the free radical method to prepare the conjugate. The formation of pcRPI-EGCG conjugates was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation chromatography, which showed that the calculated weight-average molar masses of native-pcRPI and pcRPI-EGCG conjugates were 86.9 and 215.3 kDa, respectively. The results of fluorescence, ultraviolet, circular and infrared spectra indicated that the conjugation of EGCG with native-pcRPI changed the secondary and tertiary structure of native-pcRPI. The pcRPI-EGCG conjugates exhibited higher thermal stability than native-pcRPI. The radical scavenging and reducing power of native-pcRPI were increased by 2.0-2.5-and 1.4-fold, respectively, after the EGCG-grafting reaction.CONCLUSION: These results indicate that the binding of pcRPI and EGCG effectively improved the antioxidant properties and structural characteristics of the pcRPI.

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Section: Secondary Structure Of Pcrpi-egcgsupporting

confidence: 49%

Conjugation of (–)‐epigallocatechin‐3‐gallate and protein isolate from large yellow croaker (Pseudosciaena crocea) roe: improvement of antioxidant activity and structural characteristics

Han

Yin

et al. 2021

J Sci Food Agric

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“…βLg is inherently resistant to gastric digestion due to its stability at acidic pH and its compact structure (Deng et al ., 2020). As βLg unfolded after conjugation with EGCG, its resistance to pepsin was weakened (Dönmez et al ., 2020). Moreover, ultrasound increased the gastric digestibility of βLg‐EGCG conjugates because the secondary structure of βLg in the conjugates changed more, loosening the protein and exposing more peptide bonds (Liang et al ., 2021).…”

Section: Resultsmentioning

confidence: 99%

Influence of ultrasound on the mechanism of conjugation between epigallocatechin gallate and β‐lactoglobulin for function improvement

Shao

Wang

et al. 2022

Int J of Food Sci Tech

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Epigallocatechin-3-gallate (EGCG) can improve functional properties of β-Lactoglobulin (βLg) by conjugation. The effects of ultrasound, a useful tool in improving the conjugation efficiency, on the various functional properties of βLg-EGCG conjugates were investigated in this study. Results revealed βLg-EGCG conjugates transformed from cross-linked state to fragments of various forms with ultrasound treatment with increased of binding affinity. Meanwhile, ultrasound increased secondary structural changes in βLg in the conjugated state. Functionally, ultrasound decreased the immunoglobulin E (IgE) combing capacity of βLg and enhanced its antioxidant properties by promoting the interaction between EGCG and βLg. Moreover, ultrasound induced an increase in hydrodynamic diameter and lowered the absolute zeta potential of βLg-EGCG conjugates, resulting in an increase in digestibility, as well as foaming and emulsification abilities, while, a decrease in thermal stability. The results provide valuable information for obtaining βLg with better functional properties for industrial production by ultrasoundassisted covalent binding of EGCG with βLg.

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“…The generation of new conjugate via covalent interaction between peptides and quinones of EGCG likely occurred. Also, dehydrogenation of EGCG could occur under alkaline pH, resulting in the dimerization of EGCG with augmented ability in radical scavenging activity (Dönmez et al., 2020). When comparing the antioxidative activities between S‐liposome and UAP‐liposome, the former exhibited lower activities than the latter ( p < .05), suggesting that liposome prepared using ultrasonication had higher stability in preventing the loss in antioxidant activities of conjugate than that prepared using sonication, especially under gastric condition.…”

Section: Resultsmentioning

confidence: 99%

Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

et al. 2021

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Hydrolyzed collagen (HC) from defatted sea bass skin conjugated with 3% epigallocatechin gallate (EGCG) was prepared and the resulting HC–EGCG conjugate at various levels (0.25%–2%, w/v) was loaded into liposome. The obtained liposomes were subjected to sonication (S). Liposome loaded with 1% conjugate showed the highest encapsulation efficiency (EE) (p < .05). When the ultrasound‐assisted process (UAP) at different amplitudes (20% and 40%) and times (2, 5, 10, and 15 min) were implemented, the highest EE of conjugate‐loaded liposome was found at 20% amplitude for 2 min (p < .05). When S‐liposome and UAP‐liposome were lyophilized, decreasing EE of both samples was observed (p < .05). Lyophilized UAP‐liposome had higher stability than lyophilized S‐liposome during storage at 25℃ for 28 days. Additionally, antioxidant activity in the gastrointestinal track model system (GIMs) and digest obtained from GIMs were higher for UAP‐liposome (p < .05). Therefore, liposome can be used for the delivery of conjugate. Practical applications HC from defatted sea bass skin is considered to possess several bioactivities, especially skin nourishment and bone strengthening. Nevertheless, antioxidant activity, related to the treatment of several ailments, is still low for HC. Thus, grafting of HC with polyphenol such as EGCG via free radical method can be used for the enhancement of the antioxidant activity of HC. Although the resulting conjugate has augmented activity, it is unstable during storage and in the gastrointestinal digestion system. Liposome is a promising means to stabilize the conjugate under harsh condition, especially with the aid of the UAP. Thus, liposome loaded with conjugate having the reduced size has higher antioxidant activity with increased stability, which can have a wider range of applications.

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Modulation of gastrointestinal digestion of β-lactoglobulin and micellar casein following binding by (−)-epigallocatechin-3-gallate (EGCG) and green tea flavanols

Abstract: Milk proteins bind flavanoids (EGCG and green tea extract), affecting their in vitro digestibility and modulating flavanoid free radical scavenging.

Cited by 18 publications

References 52 publications

Conjugation of (–)‐epigallocatechin‐3‐gallate and protein isolate from large yellow croaker (Pseudosciaena crocea) roe: improvement of antioxidant activity and structural characteristics

Conjugation of (–)‐epigallocatechin‐3‐gallate and protein isolate from large yellow croaker (Pseudosciaena crocea) roe: improvement of antioxidant activity and structural characteristics

Influence of ultrasound on the mechanism of conjugation between epigallocatechin gallate and β‐lactoglobulin for function improvement

Effects of sonication and ultrasound on properties and bioactivities of liposomes loaded with hydrolyzed collagen from defatted sea bass skin conjugated with epigallocatechin gallate

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