Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase

Hu, Rongkuan; Yan, Hong‐Bin; Fei, Xiaoyan; Liu, Haiyang; Wu, Jiarui

doi:10.1038/s41598-017-00925-y

Cited by 27 publications

(12 citation statements)

References 33 publications

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“…Several drugs have been widely reported to activate AMPK. For instance, metformin significantly enhances AMPK phosphorylation and regulates glycometabolism, and rosiglitazone reduces blood sugar levels and increases AMPK expression [23]. Previous research has shown that the expression of AMPK gene is downregulated in the db/db mice [24], and our results were consistent with this finding.…”

Section: Discussionsupporting

confidence: 90%

Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1

Yang

Zhang

Liu

et al. 2019

BMC Complement Altern Med

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Background Tomato fruit ( Lycopersicon esculentum Mill.) has been suggested to be useful for the prevention of diabetes. Esculeoside A is the main saponin compounds in tomatoes. This study investigated the hypoglycemic effects and the underlying mechanism of esculeoside A in C57BLKS/Leprdb ( db/db ) mice. Methods Wild-type C57BLKS ( db/dm ) mice were used in the db/dm mouse group and db/db mice were randomly divided into 2 groups: untreated and treated db/db mouse groups. Esculeoside A (100 mg/kg) was administered by gavage for 56 days to the treated db/db mouse group. Distilled water was administered to the db/dm mouse group and the untreated db/db mouse group. The blood and liver biochemical parameters and the expression of liver insulin signaling-related proteins were examined. Results The results showed that esculeoside A reduced the fasting blood glucose (FBG) levels and improved the glucose tolerance. Further investigation revealed that hepatic protein expressions of total AMP-activated protein kinase (T-AMPK), phosphorylated AMP-activated protein kinase (p-AMPK), insulin receptor substrate-1 (IRS-1), and glucokinase (GCK) were significantly upregulated after esculeoside A treatment. In contrast, the hepatic protein expression of phosphoenolpyruvate carboxykinase (PEPCK) was significantly downregulated by esculeoside A treatment. Conclusion These findings suggested that esculeoside A has a potential of alleviating the metabolic abnormalities in db/db mice via regulation of AMPK/IRS-1 pathway. Our findings supported a possible application of esculeoside A as a functional supplement for diabetes treatment.

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Section: Discussionsupporting

confidence: 90%

Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1

Yang

Zhang

Liu

et al. 2019

BMC Complement Altern Med

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“…Several phytochemicals such as resveratrol, quercetin, epigallocatechin‐3‐gallate, and curcumin suppress mitochondrial ATP production to activate AMPK. They have been reported to inhibit the mitochondrial F 1 F 0 ‐ATPase/ATP synthase, 50 whereas other AMPK activators, including berberine and shizukaol F are associated with the inhibition of respiratory chain complex I 51 . Here, we found that the treatment of CO‐EtOAc inhibited mitochondrial membrane potential and cellular respiration.…”

Section: Discussionmentioning

confidence: 46%

Chinese olive ( Canarium album L.) fruit regulates glucose utilization by activating AMP‐activated protein kinase

Yeh

Lian

et al. 2020

FASEB j.

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A growing body of evidence demonstrates obesity‐induced insulin resistance is associated with the development of metabolic diseases. This study was designed to investigate ethyl acetate fraction of Chinese olive fruit extract (CO‐EtOAc)‐mediated attenuation of obesity and hyperglycemia in a mouse model. About 60% HFD‐fed mice were treated intragastrically with CO‐EtOAc for last 6 weeks, and body weight, blood biochemical parameters as well as hepatic inflammation response were investigated. Our results showed that CO‐EtOAc treatment significantly reduced the formation of hepatic lipid droplets, body weight gain, blood glucose, and improved serum biochemical parameters in HFD‐induced obese and insulin resistant mice. We further explored the molecular mechanism underlying the blood glucose modulating effect of CO‐EtOAc using L6 myotubes model. We conclude that CO‐EtOAc effectively increases the glycogen content and glucose uptake by stimulating the membrane translocation of glucose transporter 4. In addition, CO‐EtOAc depolarizes the mitochondrial membrane and decreases the mitochondrial oxygen consumption, which may result in AMPK activation and the consequent mitochondrial fission. This study shows that CO‐EtOAc prevents the development of obesity in mice fed with HFD and is also capable of stimulating glucose uptake. The possible mechanism might be due to the effects of CO‐EtOAc on activation of AMPK and promotion of mitochondrial fission.

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“…The exosomes in the cell culture medium and in patient serum were extracted using the Total Exosome Isolation Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. The resulting precipitate was observed under a transmission electron microscope as described previously[17].…”

Section: Methodsmentioning

confidence: 99%

Mechanism of exosomal microRNA-224 in development of hepatocellular carcinoma and its diagnostic and prognostic value

Yao¹,

Xu²,

Liu³

et al. 2019

WJG

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BACKGROUND Exosomes contain proteins, lipids, and biological molecules such as DNA and RNA. Nucleic acids in exosomes are a group of molecules that can act as biomarkers. Currently, there are many reports on exosomal microRNAs, which are ideal biomarkers for the early diagnosis of cancer. However, there are few reports on the role of exosomal microRNAs in the diagnosis and prognosis of hepatocellular carcinoma (HCC). AIM To understand the mechanism of exosomal microRNA-224 (miR-224) in the development of HCC and evaluate its diagnostic and prognostic value. METHODS Cell culture and transfection of exosomal miRNA-224, real-time quantitative PCR, luciferase reporter assay, and other methods were used to find new biomarkers related to the development of HCC that can be used to diagnose HCC and predict HCC prognosis. RESULTS By targeting glycine N-methyltransferase, incubating exosomes with miR-224 mimic resulted in a significant increase in cell proliferation compared to that of the control group, while incubation with the miR-224 inhibitor significantly reduced cell proliferation. The same results were obtained for the cell invasion assay. Serum exosomal miR-224 did have some ability to differentiate patients with HCC from healthy controls, with an area under the curve of 0.910, and HCC patients with higher serum exosomal miR-224 expression had lower overall survival. CONCLUSION Exosomal miR-224 is a tumor promotor and can be a marker of diagnosis and prognosis of HCC patients, however, its ability to distinguish liver diseases needs further verification.

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Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase

Cited by 27 publications

References 33 publications

Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1

Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1

Chinese olive ( Canarium album L.) fruit regulates glucose utilization by activating AMP‐activated protein kinase

Mechanism of exosomal microRNA-224 in development of hepatocellular carcinoma and its diagnostic and prognostic value

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