Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide–Peptide Interaction

Abstract

Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders. This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)−peptide interaction. In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria. ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae. Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca 2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers. This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.

A Fermented Food Product Containing Lactic Acid Bacteria Protects ZDF Rats from the Development of Type 2 Diabetes

“…It was reported some positive correlations between Blautia spp . and microbial products such as long-chain triglycerides [70] and short chain fatty acids (SCFAs) [71], and parameters like BFG, insulin level, HOMA-IR, and weight loss [56,72]. A decreased abundance of Blautia coccoides / Eubacterium rectale was found in T1D children and was linked to intestinal disintegrity [73], while a randomized crossover study in healthy adults reported a reduced abundance of Blautia genus after prebiotic supplementation [74].…”

The Functional Interplay between Gut Microbiota, Protein Hydrolysates/Bioactive Peptides, and Obesity: A Critical Review on the Study Advances

“…Soybean also contains bioactive peptides or hydrolysates with positive impacts on body weight, and their effects on gut microbiota composition in obesity have been reported. Research investigating the modulatory effects of pepsin-released peptides of soybean 7S globulin on gut microbiota indicated that soybean 7S globulin peptide is involved in lipopolysaccharide-peptide interaction effects of gut microbiota functions related to obesity [ 49 ]. The soybean 7S globulin selectively suppressed pro-inflammatory gram-negative bacteria, and also promoted the production of SCFAs due to increased Lachnospiraceae and Lactobacillaceae relative abundance [ 49 ].…”

“…Research investigating the modulatory effects of pepsin-released peptides of soybean 7S globulin on gut microbiota indicated that soybean 7S globulin peptide is involved in lipopolysaccharide-peptide interaction effects of gut microbiota functions related to obesity [ 49 ]. The soybean 7S globulin selectively suppressed pro-inflammatory gram-negative bacteria, and also promoted the production of SCFAs due to increased Lachnospiraceae and Lactobacillaceae relative abundance [ 49 ]. Furthermore, a high amount of 7S globulin in soy milk promoted the growth of the Bacteroides-Prevotella group in obese men [ 50 ], while soybean pepsin-hydrolysate conglycinin maintained a healthy gut microbial community by inhibiting the growth of E. coli in mice [ 51 ].…”

High hydrostatic pressure treatment reduces the potential antigenicity of β‐conglycinin by changing the protein structure during in vitro digestion

“…The benefits of ⊎-conglycinin in reducing obesity and metabolic diseases have attracted the attention of researchers. [3][4][5] However, as a most important protein allergen, ⊎-conglycinin induces food allergic reactions in humans. 6 Therefore, reduction or even complete elimination of sensitization of ⊎-conglycinin will expand its utilization in the food industry.…”

“…β‐Conglycinin is the main storage protein in soybeans, and it accounts for 40% of total soybean protein. The benefits of β‐conglycinin in reducing obesity and metabolic diseases have attracted the attention of researchers 3‐5 . However, as a most important protein allergen, β‐conglycinin induces food allergic reactions in humans 6 .…”