Modulation of host defence against bacterial and viral infections by omega-3 polyunsaturated fatty acids

Husson, Marie-Odile; Ley, Delphine; Portal, Céline; Gottrand, Madeleine; Hueso, Thomas; Desseyn, Jean‐Luc; Gottrand, F.

doi:10.1016/j.jinf.2016.10.001

Cited by 83 publications

(85 citation statements)

References 101 publications

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“…Despite the fact of both ZIKV and DHA effects on neuronal cells have already been elucidated, there is nothing exploring how they could influence each other, once ZIKV and DHA have opposite effects on the same cell type 12,22 . For this reason, the present study is a pioneer in the neuroprotection field provided by DHA against ZIKV infection, and, at the same time, appends to the scarce literature about this fatty acid effects on diverse infections 27,28 .…”

Section: Discussionmentioning

confidence: 92%

Potential neuroprotective and anti-inflammatory effects provided by omega-3 (DHA) against Zika virus infection in human SH-SY5Y cells

Braz-de-Melo

Pasquarelli-do-Nascimento

Corrêa

et al. 2019

Sci Rep

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Zika virus (ZIKV) has a strong tropism for the nervous system and has been related to post-infection neurological syndromes. Once neuronal cells are infected, the virus is capable of modulating cell metabolism, leading to neurotoxicity and cellular death. The negative effect of ZIKV in neuron cells has been characterized. However, the description of molecules capable of reversing these cytotoxic effects is still under investigation. In this context, it has been largely demonstrated that docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid, is highly neuroprotective. Here, we hypothesized that DHA's neuroprotective proprieties could have an influence on ZIKV-induced neurotoxicity in SH-SY5Y cells. Our data showed that pre-treatment of SH-SY5Y cells with DHA increased the cell viability and proliferation in ZIKV-infected cells. Moreover, DHA triggered an anti-inflammatory response in those infected cells. Besides, DHA was capable of restoring mitochondria function and number in ZIKVinfected SH-SY5Y cells. In addition, cells pre-treated with DHA prior to ZIKV infection presented a lower viral load at different times of infection. Taking together, these results demonstrated that DHA has a potential anti-inflammatory and neuroprotective effect against ZIKV infection in these neuron-like cells and could be a useful tool in the treatment against this virus.

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Section: Discussionmentioning

confidence: 92%

Potential neuroprotective and anti-inflammatory effects provided by omega-3 (DHA) against Zika virus infection in human SH-SY5Y cells

Braz-de-Melo

Pasquarelli-do-Nascimento

Corrêa

et al. 2019

Sci Rep

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“…Arachidonic acid (AA), an omega-6 series PUFA, is a major component of phospholipids in the cell membrane and is important for maintaining the production function of eicosanoids. Linoleic acid (LA), an omega-6 series PUFA, and -linolenic acid (ALA), an omega-3 series as arrhythmia and chronic heart failure [CHF]), and immune system (including rheumatoid arthritis and psoriasis), and in the defense against infections [7][8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Prevention of Cardiovascular Events with Omega-3 Polyunsaturated Fatty Acids and the Mechanism Involved

Watanabe

Tatsuno

2020

JAT

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The official journal of the Japan Atherosclerosis Society and the Asian Pacific Society of Atherosclerosis and Vascular Diseases Review PUFA, are considered essential fatty acids because they cannot be synthesized by humans. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are representatives of omega-3 PUFA, and they can be converted from ALA in vivo. EPA and DHA are attracting attention due to their anti-arteriosclerotic effect. This article overviews the knowledge on the prevention of cardiovascular (CV) events with omega-3 PUFA and the mechanisms involved, focusing on the history of research on the anti-arteriosclerotic effect of fish oil. History of Fish Oil and Lifestyle-Related Diseases Mortality rate from atherosclerotic cardiovascular diseases (CVD), particularly myocardial infarction Copyright©2019 Japan Atherosclerosis Society This article is distributed under the terms of the latest version of CC BY-NC-SA defined by the Creative Commons Attribution License.

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“…The impact of polyunsaturated fatty acids on human health during bacterial infection focuses on inflammation. α-linolenic acid is converted in arachidonic acid-derived inflammatory eicosanoids (52)(53)(54). Omega-3 fatty acids are converted as well to bioactive mediators, termed specialized pro-resolving mediators, that actively reprogram the host immune response to limit inflammation (55).…”

Section: Discussionmentioning

confidence: 99%

The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagicEscherichia coli

Cazzola

Lemaire

Acket

et al. 2020

Preprint

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AbstractEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborne gastrointestinal illness. The adhesion of EHEC on host tissues is the first step enabling bacterial colonization. Adhesins like fimbriae and flagella mediate this mechanism. Here, we studied the interaction of the bacterial flagellum with the host cell’s plasma membrane using Giant Unilamellar Vesicles (GUVs) as a biologically relevant model. Cultured cell lines contain many different molecular components including proteins and glycoproteins. In contrast, with GUVs we can characterize the bacterial mode of interaction solely with a defined lipid part of the cell membrane. Bacterial adhesion on GUVs was dependent on the presence of the flagellar filament and its motility. By testing different phospholipid head groups, the nature of the fatty acid chains or the liposome curvature, we found that lipid packing is a key parameter to enable bacterial adhesion. Using HT-29 cells grown in the presence of polyunsaturated fatty acid (α-linolenic acid) or saturated fatty acid (palmitic acid), we found that α-linolenic acid reduced adhesion of wild type EHEC but not of a non-flagellated mutant. Finally, our results reveal that the presence of flagella is advantageous for the bacteria to bind to lipid rafts. We speculate that polyunsaturated fatty acids prevent flagellar adhesion on membrane bilayers and play a clear role for optimal host colonization. Flagella-mediated adhesion to plasma membranes has broad implications to host-pathogen interactions.ImportanceBacterial adhesion is a crucial step to allow bacteria to colonize their hosts, invade tissues and form biofilm. Enterohemorrhagic E. coli O157:H7 is a human pathogen and the causative agent of diarrhea and hemorrhagic colitis. Here, we use biomimetic membrane models and cell lines to decipher the impact of lipid content of the plasma membrane on enterohemorrhagic E. coli flagella-mediated adhesion. Our findings provide evidence that polyunsaturated fatty acid (α-linolenic acid) inhibits E. coli flagella adhesion to the plasma membrane in a mechanism separate from its antimicrobial and anti-inflammatory functions. In addition, we confirm that cholesterol-enriched lipid microdomains, often called lipid rafts are important in bacterial adhesion. These findings significantly strengthen plasma membrane adhesion via bacterial flagella in an important human pathogen. This mechanism represents a promising target for the development of novel anti-adhesion therapies.

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Modulation of host defence against bacterial and viral infections by omega-3 polyunsaturated fatty acids

Cited by 83 publications

References 101 publications

Potential neuroprotective and anti-inflammatory effects provided by omega-3 (DHA) against Zika virus infection in human SH-SY5Y cells

Potential neuroprotective and anti-inflammatory effects provided by omega-3 (DHA) against Zika virus infection in human SH-SY5Y cells

Prevention of Cardiovascular Events with Omega-3 Polyunsaturated Fatty Acids and the Mechanism Involved

The impact of plasma membrane lipid composition on flagella-mediated adhesion of enterohemorrhagicEscherichia coli

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