Modulation of human cord blood progenitor cell growth by recombinant human interleukin 3 (IL‐3), IL‐6, granulocyte‐macrophage colony stimulating factor (GM‐CSF) and stem cell factor (SCF) in serum‐supplemented and serum‐free medium

Bruyn, Cécile De; Delforge, Alain; Bron, Dominique; Ley, P.; Hemptinne, D. de; Stryckmans, Pierre

doi:10.1002/stem.5530120609

Cited by 10 publications

(9 citation statements)

References 27 publications

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“…Previous studies have demonstrated that HUCB HPC seem to be more primitive than their counterparts in BM or peripheral blood [32][33][34][35][36][37][38][39][40][41][42][43][44]. In the present study, we have demonstrated that the anti-HIV agents IFN-␣ and ARA-C have a greater effect on BM HPC than HUCB HPC.…”

Section: Discussionsupporting

confidence: 58%

“…The hematopoietic progenitors in HUCB seem to be more primitive than their counterparts in BM or peripheral blood: The colonies in agar or methyl cellulose are bigger and the multipotential colony-forming units (CFU-Mix) and highly proliferative potential colony-forming cells (HPP-CFC) are more numerous. Compared to adult BM stem cells, the cells survive longer and expand with different growth factors in longterm cultures [32][33][34][35][36][37][38]. Integration of retroviruses or adeno-associated viruses is more effective in HUCBenriched progenitor cells than in adult BM [39][40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

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Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV‐infected newborns

Toren¹,

Einat²,

Fabian³

et al. 1997

Am. J. Hematol.

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Vertical transmission from mother to child occurs in 15-39% of women infected with the human immunodeficiency virus (HIV). Stem cell transplantation has recently been suggested as a potential therapy for patients with HIV infection. We have examined the possible advantages of human cord blood (HUCB) stem cells over bone marrow (BM) stem cells in the treatment of HIV-infected newborns. HUCB myeloid progenitors were found to be statistically more resistant to interferon-␣ (IFN-␣), cytarabine (ARA-C), and eilatin than BM myeloid progenitor cells grown with IL-3 (P < 0.05). HUCB treated with IFN-␣, ARA-C, and eilatin demonstrated a significantly higher capacity for self-renewal manifested by delta assay following 7 days in liquid culture. We, therefore, suggest that HUCB purged by anti-HIV drugs may be a source for autologous transplantation in HIVinfected newborns. Am.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV‐infected newborns

Toren¹,

Einat²,

Fabian³

et al. 1997

Am. J. Hematol.

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“…We considered that to be useful for adult stem cell transplantation, a CB sample should be expanded by at least fourfold (11). In the present study, only 11 of 23 CB samples responded with such an expansion.…”

Section: Discussionmentioning

confidence: 83%

“…The expansion of progenitor cells was heterogeneous, with CD34+ cell expansion ranging from 0 to 309-fold and CD34+CD38-cell expansion ranging from 0 to 45fold. Considering that a nonexpanded CB sample can be used to safety transplant a 20-kg body recipient (11), the progenitor cell expansion required for an acceptable adult graft would be in excess of fourfold. We have chosen, in this study, to take into account for evaluation of the expansion only CB samples in which the CD34+ cell expansions were greater than fourfold and thus could be useful for clinical application.…”

Section: Resultsmentioning

confidence: 99%

“…It is particularly essential for the expansion of cord blood samples, for which it has already been demonstrated that the mean number of hematopoietic progenitor cells appears too low to safely engraft an adult patient (11). However, to date, the exact number of immature cord blood progenitor cells that would be required for a graft has not been determined.…”

Section: Introduction When Exposed To Various Cocktails Of Cy-mentioning

confidence: 99%

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Ex Vivo Expansion of CD34+CD38− Cord Blood Cells

Bruyn

Delforge²,

Bron³

et al. 1997

Journal of Hematotherapy

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CD34+ cord blood (CB) cells were expanded in stromal cell-free long-term culture (LTC), in the presence of various combinations of interleukin-3 (IL-3), stem cell factor (SCF), IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and anti-transforming growth factor-beta (anti-TGF-beta) antibody. The progenitor cell expansion was evaluated by monitoring the increase of CD34+ and CD34 + CD38- cells over a period of 21 days. The expansion of immature (B1-CFC, HPP-CFC) and of more committed progenitors (CFU-GM, CFU-GEMM, BFU-E) was also evaluated in specific samples. Our results show that (a) CD34+ cell expansion is highly variable depending on the cord blood samples studied, (b) significant correlations between B1-CFC and CD34 + CD38- and between total CFU and CD34+ cell expansion are observed, (c) SCF in combination with IL-3 appears to expand cell subsets that continue to express their CD34 + CD38- phenotype and that generate both immature and committed progenitors, and (d) the addition of IL-6, GM-CSF, or anti-TGF-beta does not significantly improve these expansions.

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Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV-infected newborns

et al. 1997

View full text Add to dashboard Cite

Vertical transmission from mother to child occurs in 15-39% of women infected with the human immunodeficiency virus (HIV). Stem cell transplantation has recently been suggested as a potential therapy for patients with HIV infection. We have examined the possible advantages of human cord blood (HUCB) stem cells over bone marrow (BM) stem cells in the treatment of HIV-infected newborns. HUCB myeloid progenitors were found to be statistically more resistant to interferon-alpha (IFN-alpha), cytarabine (ARA-C), and eilatin than BM myeloid progenitor cells grown with IL-3 (P < 0.05). HUCB treated with IFN-alpha, ARA-C, and eilatin demonstrated a significantly higher capacity for self-renewal manifested by delta assay following 7 days in liquid culture. We, therefore, suggest that HUCB purged by anti-HIV drugs may be a source for autologous transplantation in HIV-infected newborns.

show abstract

Modulation of human cord blood progenitor cell growth by recombinant human interleukin 3 (IL‐3), IL‐6, granulocyte‐macrophage colony stimulating factor (GM‐CSF) and stem cell factor (SCF) in serum‐supplemented and serum‐free medium

Cited by 10 publications

References 27 publications

Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV‐infected newborns

Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV‐infected newborns

Ex Vivo Expansion of CD34+CD38− Cord Blood Cells

Human umbilical cord blood myeloid progenitor cells are relatively chemoresistant: A potential model for autologous transplantations in HIV-infected newborns

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