Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

Abstract

Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol–gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior.

Effects of fluoridation of porcine hydroxyapatite on osteoblastic activity of human MG63 cells

“…At predetermined periods of time, the cells on FPHA were washed three times with PBS and fixed in 3.7% formaldehyde solution in PBS for 10 min at room temperature. Then 0.1% Triton-X100 PBS was added to increase cell membrane permeability, and 1% bovine serum albumin was added to reduce nonspecific background staining [23]. Finally, cells were stained with rhodamine-phalloidin 635 (Life technologies, Invitrogen, USA) for actin filaments and Hoechst 33342 (Sigma-Aldrich, USA) for nuclei.…”

A parametric study of laser interference surface patterning of dental zirconia: Effects of laser parameters on topography and surface quality

“…Common strategies to achieve these goals are the modification of the roughness or the introduction of a controlled topography. For instance, micrometric silica reliefs on zirconia implant surfaces have been employed to study endothelial cells and fibroblast response in [8], where it was demonstrated that parallel grooved surfaces were able to control cells spatial distribution and to guide their growth. Also an increment in roughness has proved to be beneficial in the osseointegration of zirconia-based implants [9].…”

Assessment of Streptococcus Mutans Adhesion to the Surface of Biomimetically-Modified Orthodontic Archwires

“…Different alternatives have been proposed to reduce bacterial adhesion to the surface of biomaterials. Among such alternatives, physical surface modifications, including roughening of zirconia surfaces [9] or modification of the topography of materials to observe cellular [10,11] or bacterial behavior [12], have received attention in the last years.…”