Modulation of human sperm function by follicular fluid*

Munuce, María José; Caille, Adriana; Botti, Gustavo; Berta, C. L.

doi:10.1111/j.1439-0272.2004.00638.x

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…Human follicular fluid reduced the zona binding capacity of human spermatozoa in vitro (16,17,33). Glycodelin-F is the main factor contributing to the inhibitory activity of human follicular fluid (12), although glycodelin-A is also present in the follicular fluid in a much lesser amount.…”

Section: Discussionmentioning

confidence: 99%

Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding

Chiu

Chung

Koistinen

et al. 2007

Journal of Biological Chemistry

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Spermatozoa have to swim through the oviduct and the cumulus oophorus before fertilization in vivo. In the oviduct, spermatozoa are exposed to glycodelin-A and -F that inhibit spermatozoa-zona pellucida binding. In this study, we determined whether these glycodelins would inhibit fertilization. The data showed that the spermatozoa without previous exposure to glycodelin-A and -F acquired glycodelin immunoreactivity during their passage through the cumulus oophorus. On the other hand, when glycodelin-A or -F-pretreated spermatozoa were exposed to the cumulus oophorus, the zona pellucida binding inhibitory activity of glycodelin-A and -F was not only removed, but the spermatozoa acquired enhanced zona pellucida binding ability. These actions of the cumulus oophorus were due to the presence of a cumulus isoform of glycodelin, designated as glycodelin-C. The cumulus cells could convert exogenous glycodelin-A and -F to glycodelin-C, which was then released into the surrounding medium. The protein core of glycodelin-C was identical to that in other glycodelin isoforms, as demonstrated by mass spectrum, peptide mapping, and affinity to anti-glycodelin antibody recognizing the protein core of glycodelin. In addition to having a smaller size and a higher isoelectric point, glycodelin-C also had lectin binding properties different from other isoforms. Glycodelin-C stimulated spermatozoazona pellucida binding in a dose-dependent manner, and it effectively displaced sperm-bound glycodelin-A and -F. In conclusion, the cumulus cells transform glycodelin-A and -F to glycodelin-C, which in turn removes the spermatozoazona binding inhibitory glycodelin isoforms and enhances the zona binding capacity of spermatozoa passing through the cumulus oophorus.

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Section: Discussionmentioning

confidence: 99%

Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding

Chiu

Chung

Koistinen

et al. 2007

Journal of Biological Chemistry

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“…Since in vitro fertilization techniques make possible the recovery of human FF, several studies aimed to determine its effects on human sperm function (summarized by 42 ). Our observations agree with a previous work in which no modification in the Tyr-P signal of human sperm was observed in the presence of FF, even though this fluid was used at a higher concentration 42 . Interestingly, the ~ 87 kD species exhibited a lower degree of phosphorylation in the presence of FF.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide-targeted protein phosphorylation during human sperm capacitation

Staicu

Martínez-Soto

Cánovas

et al. 2021

Sci Rep

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Among many other molecules, nitric oxide insures the correct progress of sperm capacitation by mediating phosphorylation events. For a more comprehensive understanding of how this happens, we capacitated human spermatozoa from healthy men in the presence/absence of S-Nitrosoglutathione, a nitric oxide donor, two nitric oxide synthase inhibitors, NG-Nitro-l-arginine Methyl Ester Hydrochloride and Aminoguanidine Hemisulfate salt and, finally, with/without l-Arginine, the substrate for nitric oxide synthesis, and/or human follicular fluid. When analyzing the phosphorylation of protein kinase A substrates and tyrosine residues, we particularly observed how the inhibition of nitric oxide synthesis affects certain protein bands (~ 110, ~ 87, ~ 75 and ~ 62 kD) by lowering their phosphorylation degree, even when spermatozoa were incubated with l-Arginine and/or follicular fluid. Mass spectrometry analysis identified 29 proteins in these species, related to: spermatogenesis, binding to the zona pellucida, energy and metabolism, stress response, motility and structural organization, signaling and protein turnover. Significant changes in the phosphorylation degree of specific proteins could impair their biological activity and result in severe fertility-related phenotypes. These findings provide a deeper understanding of nitric oxide’s role in the capacitation process, and consequently, future studies in infertile patients should determine how nitric oxide mediates phosphorylation events in the species here described.

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“…Adicionalmente, Munuce et al (2004) concluían que el fluido folicular reducía la capacidad fecundante en espermatozoides humanos, aunque en este último trabajo, hubo una prolongada capacitación previa con BSA, lo que podría haber disminuido la longevidad de los espermatozoides. Contrariamente a estos trabajos, en el presente estudio, se demuestra que el fluido folicular de alpaca estimula la unión de los espermatozoides a la zona pelúcida de esta especie, lo que, además, evidenciaría que el fluido folicular de alpaca capacita a los espermatozoides, ya que sólo los espermatozoides capacitados pueden unirse a la zona pelúcida (Graham y Mocé, 2005;Oehninger et al, 2014).…”

Section: Discussionunclassified

EFECTO DEL FLUIDO FOLICULAR SOBRE LA CAPACIDAD DE UNIÓN A ZONA PELÚCIDA DE ESPERMATOZOIDES EPIDIDIMARIOS DE ALPACA (Vicugna pacos)

Bravo¹,

Rubén²,

Valdivia³

2017

SPERMOVA

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Follicular fluid is the liquid fraction that surrounds the oocyte during its growth and maturation in the ovary. The components of the follicular fluid can favor the functionality of the sperm. In the present research, the binding to the zona pellucida of epididymal spermatozoa of alpaca incubated with follicular fluid was studied. In the experiment a control group and a treatment group were used. The sperm from the control group and the treatment were incubated with zona pellucidae. The incubation of the treatment group was performed in the presence of follicular fluid at a concentration of 30%; while the incubation of the control group occurred in the absence of follicular fluid. It was observed that a greater number of spermatozoa joined the zona pellucida in the group incubated in the presence of follicular fluid. It was found that there were statistical differences between treatment and control. It is concluded that alpaca follicular fluid enhances the in vitro binding of spermatozoa to the zona pellucida of this species.Keywords: Alpaca, follicular fluid, zona pellucida, epididymal sperm, gametic interaction. RESUMENEl fluido folicular es la fracción líquida que rodea al ovocito durante su crecimiento y maduración en el ovario. Los componentes del fluido folicular pueden favorecer la funcionalidad de los espermatozoides. En el presente trabajo se ha estudiado la unión a zona pelúcida de espermatozoides epididimarios de alpaca incubados con fluido folicular de esta especie. En el experimento se utilizó un grupo control y un grupo tratamiento. Los espermatozoides del grupo control y el tratamiento fueron incubados con zonas pelúcidas. La incubación del grupo tratamiento se realizó en presencia de fluido folicular a una concentración de 30%; mientras que la incubación del grupo control se dio en ausencia de fluido folicular. Se observó que un mayor número de espermatozoides se unían a la zona pelúcida en el grupo incubado en presencia de fluido folicular. Se encontró que había diferencias estadísticas entre el tratamiento y el control. Se concluye que el fluido folicular de alpaca favorece la unión in vitro de espermatozoides a la zona pelúcida de esta especie.Palabras clave: Alpaca, fluido folicular, zona pelúcida, espermatozoides epididimarios, interacción gamética.

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Modulation of human sperm function by follicular fluid*

Cited by 11 publications

References 39 publications

Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding

Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding

Nitric oxide-targeted protein phosphorylation during human sperm capacitation

EFECTO DEL FLUIDO FOLICULAR SOBRE LA CAPACIDAD DE UNIÓN A ZONA PELÚCIDA DE ESPERMATOZOIDES EPIDIDIMARIOS DE ALPACA (Vicugna pacos)

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