Modulation of laccase transcriptome during biodegradation of naphthalene by white rot fungus Pleurotus ostreatus

Elhusseiny, Shaza M.; Amin, Heba M.; Shebl, Rania Ibrahim

doi:10.1007/s10123-018-00041-5

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…Although esterase activity in the early stages of PAH mineralization has been reported for different fungi (Pozdnyakova et al ., 2010; González‐Abradelo et al ., 2019), our transcriptomic data did not show overexpression of these genes in the presence of PAHs in A. sydowii . In contrast, high transcriptomic expression of laccases was observed previously in Pleurotus ostreatus in the presence of naphthalene (Elhusseiny et al ., 2018), and in T. verruculosus in the presence of pyrene or Phe (Gao et al ., 2019). Taken together, the transcriptional and enzymatic profiles of A. sydowii confirmed previous observations on the lack of correlation between LME production and PAH biodepletion in several ascomycetes (e.g.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Peidro-Guzmán

Pérez-Llano

González-Abradelo

et al. 2020

Environmental Microbiology

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Polycyclic aromatic hydrocarbons (PAHs) are among the most persistent xenobiotic compounds, with high toxicity effects. Mycoremediation with halophilic Aspergillus sydowii was used for their removal from a hypersaline medium (1 M NaCl). A. sydowii metabolized PAHs as sole carbon sources, resulting in the removal of up to 90% for both PAHs [benzo [a] pyrene (BaP) and phenanthrene (Phe)] after 10 days. Elimination of Phe and BaP was almost exclusively due to biotransformation and not adsorption by dead mycelium and did not correlate with the activity of lignin modifying enzymes (LME). Transcriptomes of A. sydowii grown on PAHs, or on glucose as control, both at hypersaline conditions, revealed 170 upregulated and 76 downregulated genes. Upregulated genes were related to starvation, cell wall remodelling, degradation and metabolism of xenobiotics, DNA/RNA metabolism, energy generation, signalling and general stress responses. Changes of LME expression levels were not detected, while the chloroperoxidase gene, possibly related to detoxification processes in fungi, was strongly upregulated. We propose that two parallel metabolic pathways (mitochondrial and cytosolic) are involved in degradation and detoxification of PAHs in A. sydowii resulting in intracellular oxidation of PAHs. To the best of our knowledge, this is the most comprehensive transcriptomic analysis on fungal degradation of PAHs.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Peidro-Guzmán

Pérez-Llano

González-Abradelo

et al. 2020

Environmental Microbiology

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“…Mimics mammalian detoxification of naphthalene [7] Phlebia lindtneri Inhibition of PAH degradation in the presence of P-450 inhibitors [26] Phanerochaete chrysosporium Degraded PAH upto 600 mg/kg concentration in soil microcosm studies [27] Armillaria sp. F022 Dioxygenantion of 1,4 position C-atom to give naphthoquinones [28] Pleurotus eryngii Mineralize Naphthalene by dioxygenating C1−C4 atom [29] Pleurotus ostreatus Αand β-naphthol were initial degradation products which further metabolized to salycilic and benzoic acid [30] Ganoderma sp. Lower pathway metabolites benzoic acid, catechol, pthalic acid, and protocatechuic acid were obtained [31] Aspergillus Glaucus AL1 Phenol-2-monoxygenase and catechol-1,2-dioxygenase are involved in degradation [32] Phenanthrene Pleurotus ostreatus P-450 was mainly responsible for the activity [8] Irpex lacteus - [33] Ceratobasidium stevensii Degraded 89.51% of phenanthrene, phenanthrene removal, and MnP activity was not corelated [34] Polyporus sp.…”

Section: Cunninghamella Elegansmentioning

confidence: 99%

Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions

Bokade

Bajaj

2023

J Basic Microbiol

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous high global concern environmental pollutants and tend to bioaccumulate due to hydrophobic properties. These xenobiotics, having variable concentrations along different matrices, gradually undergo various physical, chemical, and biological transformation processes. Myco‐remediation aids accelerated degradation by effectively transforming complex ring structures to oxidized/hydroxylated intermediates, which can further funnel to bacterial degradation pathways. Exploitation of such complementing fungal−bacterial enzymatic activity can overcome certain limitations of incomplete bioremediation process. Furthermore, high‐throughput molecular methods can be employed to unveil community structure, taxon abundance, coexisting community interactions, and metabolic pathways under stressed conditions. The present review critically discusses the role of different fungal phyla in PAHs biotransformation and application of fungal−bacterial cocultures for enhanced mineralization. Moreover, recent advances in bioassays for PAH residue detection, monitoring, developing xenobiotics stress‐tolerant strains, and application of fungal catabolic enzymes are highlighted. Application of next‐generation sequencing methods to reveal complex ecological networks based on microbial community interactions and data analysis bias in performing such studies is further discussed in detail. Conclusively, the review underscores the application of mixed‐culture approach by critically highlighting in situ fungal−bacterial community nexus and its role in complete mineralization of PAHs for the management of contaminated sites.

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“…Based on the obtained metabolites, the researchers proposed the course of naphthalene degradation using P. ostratus and its enzymes, naphthalene dioxygenase and laccases. The final product of the reaction was benzoic acid [212], the simplest aromatic carboxylic acid with low toxicity and antibacterial and antifungal activity, most often used in the food industry and cosmetics. In addition, a higher level of the degradation of naphthalene was observed compared to anthracene and 1,10-phenanthroline, further supporting the idea that low molecular weight PAHs are more easily degraded due to their higher solubility and greater bioavailability.…”

Section: Pahs (Polycyclic Aromatic Hydrocarbons)mentioning

confidence: 99%

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Gałązka¹,

Jankiewicz²,

Szczepkowski³

2023

Preprint

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Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number of pollutants entering the environment. The current concentration of xenobiotics released into the environment exceeds its natural ability to decompose them. Enzymatic degradation of pollutants seems to be an environmentally friendly process. Due to the wide spectrum of substrate specificity, from inorganic compounds to high molecular weight organic compounds such as PAH or dyes, as well as favorable biochemical properties, laccase has been used in the biological removal of xenobiotics from the environment. It is important to understand the degradation mechanisms of pollutants and to evaluate the final products in terms of their toxicity. The laccase oxidizes the substrates with the simultaneous reduction of molecular oxygen to water, which is the purest reaction co-substrate. That is why it is called a green biocatalyst. The trend is an increase in the production of enzymes related to the intensive development of industry, bioremediation or synthetic chemistry. This leads to the search for laccases with greater activity and stability under extreme conditions. The potential of laccases to degrade xenobiotics can be promoted by improving enzymatic catalytic characterization using protein engineering and other genetic engineering methods.

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Modulation of laccase transcriptome during biodegradation of naphthalene by white rot fungus Pleurotus ostreatus

Cited by 15 publications

References 31 publications

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions

Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

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