Modulation of LPS-induced inflammation in RAW264.7 murine cells by novel isoflavonoids from Millettia pulchra

Xue, Linlin; Wu, Wenshuang; Ma, Xu; Pei, Heying; Tang, Minghai; Kuang, Shuang Yuan; Cai, Xiaoying; Wang, Lun; Li, Yan; Zhang, Ruijia; Hong, Feng; Peng, Aihua; Ye, Hao-Yu; Chen, Lijuan

doi:10.1016/j.bioorg.2020.103693

Cited by 13 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were reported in other food-derived peptides (Ren et al, 2018;Yi et al, 2020). Cyclooxygenase enzymes-2 (COX-2) is an essential rate-limiting enzyme involved in catalyzing the biosynthesis of pro-inflammatory factor prostaglandin E2 (PGE2) from arachidonic acid (Xue et al, 2020). In this study, the mRNA expressions of INOS, COX-2, TNF-α, and the protein content of INOS were down-regulated in LPF treatment groups (Figure 4A-4D).…”

Section: Discussionsupporting

confidence: 87%

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Wang

Zhi

Han

et al. 2021

Food and Agricultural Immunology

View full text Add to dashboard Cite

The purpose of this study was to explore the anti-inflammatory mechanism of walnut peptide LPF in the LPS-induced RAW264.7 cells model. The walnut peptide LPF down-regulated the phagocytic ability and the production of nitric oxide (NO) induced by LPS-stimulated RAW264.7. Molecular docking analysis showed that the walnut peptide LPF can bind into the active pocket of inducible nitric oxide synthase (iNOS) with high affinity. Further, the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumour necrosis factor-α (TNF-α) were suppressed by the walnut peptide LPF intervention. Later, we validated the under-expression of INOS protein by western blot. The decreased mRNA expression of IκB-α demonstrated the anti-inflammatory effects of the walnut peptide LPF were related to the regulation of the NF-κB signalling pathway. Our results suggest that the walnut peptide LPF has the potential to be developed as a functional food ingredient against inflammation.

show abstract

Section: Discussionsupporting

confidence: 87%

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Wang

Zhi

Han

et al. 2021

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…The over‐secreted NO is closely linked to the inflammatory response 41 . Along with insights into the pathogenic mechanisms, many anti‐cytokines are increasingly used to regulate inflammatory diseases; for example, Sarilumab (IL‐6 inhibitor) for rheumatoid arthritis, Bindarit (MCP‐1 inhibitor) for Pneumonia and Adalimumab (TNF‐α inhibitor) for psoriasis 42 . It is an effective approach for controlling a chronic or acute inflammatory response; thus, the effects of BAE on the NO, IL‐6, MCP‐1 and TNF‐α levels were examined.…”

Section: Resultsmentioning

confidence: 99%

Blueberry anthocyanins extract inhibits advanced glycation end‐products (AGEs) production and AGEs‐stimulated inflammation in RAW264.7 cells

Peng,

Liang,

Wen

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

BackgroundPharmacological interference is considered to be a successful approach to inhibit the AGEs production, and to block AGEs‐induced diseases. Some synthetic medicines are effective for inhibiting the glycation reaction, but they can not be widely applied in clinical due to their side effects and security concerns. This is the first work to use blueberry anthocyanins extract (BAE) to attenuate advanced glycation end‐products (AGEs) formation and AGEs‐induced inflammatory response in vitro.ResultsIn bovine serum albumin (BSA)‐Glucose model, BAE showed similar inhibitory activity on AGEs compared with the synthetic anti‐glycation agent (Aminoguanidine). The results showed that BAE exhibit strong anti‐glycative action by scavenging glycosylated intermediates (Schiff base, fructosamine, and α‐dicarbonyl compounds), attenuating the molecular aggregation and amyloid‐like fibrils formation, and preventing conformational modification. Additionally, BAE was found to dose‐dependently inhibit the AGEs‐induced secretions of NO and pro‐inflammatory cytokines (IL‐6, MCP‐1, and TNF‐α) in RAW264.7 cells. The anti‐inflammation activity of BAE was mediated by down‐regulating the expressions of critical inflammatory markers, iNOS and COX‐2, through NF‐κB signaling pathways inhibition.ConclusionBAE could serve as a natural inhibitor for controlling AGEs and AGEs‐induced chronic inflammation.This article is protected by copyright. All rights reserved.

show abstract

“…Nitric oxide is a critical signaling molecule that is generated by nitric oxide synthase, and it has been confirmed to play a vital role in the development of inflammatory‐related diseases (Xue et al., 2020 ). Therefore, in the present study, anti‐inflammatory activities of BLF were estimated by detecting NO production of LPS‐stimulated RAW264.7 cells.…”

Section: Resultsmentioning

confidence: 99%

Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3‐O‐rhamnoside ameliorate lipopolysaccharide‐induced inflammation and oxidative stress in RAW264.7 cells

Cui

Zong

Zhao

et al. 2022

Food Science & Nutrition

View full text Add to dashboard Cite

In this study, the anti‐inflammatory and antioxidant activities and mechanism of burdock leaf flavonoids (BLF) on LPS‐stimulated inflammation in RAW264.7 macrophage cells were explored. We have observed that BLF and main effective components morin and quercetin 3‐O‐rhamnoside pretreatment significantly inhibited LPS‐stimulated inflammatory activation of RAW264.7 cells by lowering the levels of NO, PGE2, TNF‐α, and IL‐6 production ( p < .05). At the same time, BLF not only had potent free radical scavenging ability in vitro (DPPH: 2025.33 ± 84.15 μmol Trolox/g, ABTS: 159.14 ± 5.28 μmol Trolox/g, and ORAC: 248.72 ± 9.74 μmol Trolox/g) but also effectively ameliorated cellular oxidative stress status by restoring the decreased activity of antioxidant enzymes (SOD, CAT, and GSH‐Px) and decreasing the elevated levels of ROS and TBARS in LPS‐stimulated macrophages ( p < .05). The western blot analysis indicated that BLF and main components morin and quercetin 3‐O‐rhamnoside mainly inhibited LPS‐stimulated inflammation by reducing the iNOS and COX‐2 protein expression, decreasing cellular ROS, and blocking the activation of NF‐κB signaling pathway in macrophages. Our results collectively imply that BLF could be used as a new type of functional factor for the development of antioxidant and anti‐inflammatory foods.

show abstract

Modulation of LPS-induced inflammation in RAW264.7 murine cells by novel isoflavonoids from Millettia pulchra

Cited by 13 publications

References 41 publications

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Blueberry anthocyanins extract inhibits advanced glycation end‐products (AGEs) production and AGEs‐stimulated inflammation in RAW264.7 cells

Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3‐O‐rhamnoside ameliorate lipopolysaccharide‐induced inflammation and oxidative stress in RAW264.7 cells

Contact Info

Product

Resources

About