Modulation of matrix vesicle enzyme activity and phosphatidylserine content by ceramic implant materials during endosteal bone healing

Schwartz, Zvi; Swain, L. D.; Marshall, Thomas; Sela, J.; Groß, Ulrich; Amir, D.; Müller-Mai, C.; Boyan, Barbara D.

doi:10.1007/bf00296676

Cited by 25 publications

(14 citation statements)

References 43 publications

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“…3,4 One possible explanation for this unique response to titanium is that osteoblasts respond to titanium surfaces by secreting local and systemic factors that may act on themselves in an autocrine fashion, stimulating their own proliferation and differentiation, but may also have paracrine and endocrine effects on other cells as well. 5 In addition to the nature of the surrounding bone, hard tissue formation around implants is profoundly influenced by the surface of the implant.…”

Section: Introductionmentioning

confidence: 99%

Varying Ti‐6Al‐4V surface roughness induces different early morphologic and molecular responses in MG63 osteoblast‐like cells

Kim

et al. 2005

J Biomedical Materials Res

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Osteoblast response to Ti implants depends not only on the chemistry of the implant but also on the physical properties of the implant surface, such as microtopography and roughness. This study was undertaken to examine early changes in cell morphology and gene expression during the early phase of osteoblast interaction with titanium alloy (Ti-6Al-4V) surfaces of two different roughnesses. MG63 osteoblast-like cells were cultured for 2, 6, 24, and 72 h on smooth (R a ϭ 0.18 Ϯ0.03 m) and rough (R a ϭ 2.95 Ϯ0.23 m) Ti-6Al-4V surfaces. Changes in cell proliferation were assessed by measuring cell number after 72 h in culture. Morphological characteristics were observed by scanning electron microscopy after 2, 6, and 24 h of culture. Changes in gene expression for extracellular signal-regulated kinase 2 (Erk2), type I collagen (␣ 2 [I] collagen), phospholipase C-␥2 (Plc-␥2), and ␤-actin were measured by RT-PCR after 6 and 24 h in culture. Cell number was significantly higher on the smooth surface. In scanning electron micrographs, cells on smooth Ti-6Al-4V were spherical and raised up from the surface after 2 h in culture. In contrast, cells on the rough surface adopted an irregular, elongated shape that spanned across pits in the surface. At 24 h, cells on the smooth surface had flattened, become elongate, and covered the surface. In contrast, cells on the rough surface appeared more differentiated in shape and the margins of the cells were irregular, with many processes extending out, following the contour of the surface. Of the genes examined, only Erk2 and ␤-actin showed a change in expression with surface roughness. Both genes were upregulated (p Ͻ 0.05) on the rough surface at 6 h. These results indicate that Ti-6Al-4V surface roughness affects osteoblast proliferation, morphology, and gene expression, and that these effects can be measured after periods as short as 2-6 h.

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Section: Introductionmentioning

confidence: 99%

Varying Ti‐6Al‐4V surface roughness induces different early morphologic and molecular responses in MG63 osteoblast‐like cells

Kim

et al. 2005

J Biomedical Materials Res

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“…Implant surface character is one implant design factor affecting the rate and extent of osseointegration [14][15][16][17][18]. The process of osseointegration is now well described both histologically and at the cellular level.…”

Section: Introductionmentioning

confidence: 99%

Advancing dental implant surface technology – From micron- to nanotopography

et al. 2008

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“…There have also been reports that the osseointegration rate of a titanium implant is related to properties of the implant surface 5,6) . Accordingly, the titanium surface has been topographically or chemically modifi ed to increase the osseointegration rate [7][8][9][10] . On the other hand, hydroxyapatite is classifi ed as a bioactive material with excellent "osteoconductive" abilities [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

et al. 2012

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Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affi nity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.

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Modulation of matrix vesicle enzyme activity and phosphatidylserine content by ceramic implant materials during endosteal bone healing

Cited by 25 publications

References 43 publications

Varying Ti‐6Al‐4V surface roughness induces different early morphologic and molecular responses in MG63 osteoblast‐like cells

Varying Ti‐6Al‐4V surface roughness induces different early morphologic and molecular responses in MG63 osteoblast‐like cells

Advancing dental implant surface technology – From micron- to nanotopography

Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis

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