Modulation of methotrexate efficacy by green tea polyphenols in rat adjuvant arthritis

Poništ, Silvester; Gardi, Concetta; Pašková, Ľudmila; Švík, Karol; Slovák, Lukáš; Bilka, František; Tedesco, Idolo; Bauerová, Katarína; Russo, Gian Luigi

doi:10.1016/j.phanu.2020.100228

Cited by 9 publications

(4 citation statements)

References 45 publications

(55 reference statements)

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“…Earlier findings suggested that major clinical markers, HPV and body weight gain, are remarkably worsened due to inflammation and were effectively diminished by administration of compounds with anti-oxidative and/or anti-inflammatory properties [38][39][40]. The protective effect of MTX against AA development agrees with previously reported results [23,28,37,40,41] as well as with changes of HPV shown in Figure 3. Combination of RB with MTX did not improve these protective effect of MTX (Figures 2 and 3).…”

Section: Discussionsupporting

confidence: 88%

“…The AA is a well-established model of inflammation [20,21]. The adjuvant arthritis was induced in rats with weight of 160-180 g (6 weeks) by an individual intradermal immunization at the base of the tail with suspension of 0.1 mL of 12 mg/mL heat-inactivated MB powder suspended in incomplete IFA according to our previous protocol [22,23].…”

Section: Adjuvant Induced Arthritis In Lewis Ratsmentioning

confidence: 99%

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Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis

et al. 2021

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Anti-inflammatory potential of orally administrated bioflavonoid-robinin, active sub-stance of original drug Flaroninum™ (FL), was investigated in the combination with methotrexate (MTX) and in monotherapy in rats suffering from adjuvant-induced arthritis (AA). Robinin (kaempferol-3-O-robinoside-7-O-rhamnoside) was isolated from the aerial parts of Astragalus falcatus Lam. The monotherapy with robinin was not efficient in alleviating symptoms of AA. The combination of MTX with robinin was similarly active as MTX alone in reducing the hind paw volume and change of body weight during the whole experiment. The combination, however, reduced plasma levels of Interleukin-17Aand activity of gamma-glutamyl transferase in joint more efficiently then MTX alone. Our results demonstrate that the novel combination of robinin and MTX mildly improved the reduction of inflammation in experimental arthritis.

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Section: Discussionsupporting

confidence: 88%

Section: Adjuvant Induced Arthritis In Lewis Ratsmentioning

confidence: 99%

Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis

et al. 2021

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“…Then again, this model lacks the chronic progressive nature of RA, and the pathological changes are self-limiting [ 130 ]. Adjuvant arthritis was induced in rats with a weight of 160–180 g (6 weeks) by individual intradermal immunization at the base of the tail with a suspension of 0.1 mL of 12 mg/mL heat-inactivated Mycobacterium butyricum powder suspended in incomplete Freund’s adjuvant according to our previous protocol [ 131 , 132 ].…”

Section: Methodsmentioning

confidence: 99%

Rhodiola rosea L. Extract, a Known Adaptogen, Evaluated in Experimental Arthritis

et al. 2023

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Rhodiola rosea L. extract (RSE) is mostly known for its adaptogen properties, but not for its antiarthritic activities, therefore monotherapy and combination with low-dose methotrexate (MTX) was studied. The collagen-induced arthritis (CIA) model was used to measure the functional score, and the change in hind paw volume (HPV). Both parameters had significant antiarthritic effects. Based on these preliminary results, an adjuvant arthritis (AA) model was further applied to assess another parameters. The experiment included these animal groups: healthy controls, untreated AA, AA administered with RSE (150 mg/kg b.w. daily, p.o.), AA administered by MTX (0.3 mg/kg b.w. twice a week, p.o.), and AA treated with the combination of RSE+MTX. The combination of RSE+MTX significantly reduced the HPV and increased the body weight. The combination significantly decreased HPV when compared to MTX monotherapy. The plasmatic levels of inflammatory markers (IL-6, IL-17A, MMP-9 and CRP) were significantly decreased by MTX+RSE treatment. The RSE monotherapy didn’t influence any of the inflammatory parameters studied. In CIA, the RSE monotherapy significantly decreased the arthritic parameters studied. In summary, the combination of RSE and sub-therapeutic MTX was significantly effective in AA by improving inflammatory and arthritic parameters.

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“…Studies have shown that EGCG negatively regulates signal transduction pathways and plays an important role in arthritis and joint injury (Khan et al, 2008). In the rat model of human rheumatoid arthritis established by Ponist, it was found that the plasma monocyte chemoattractant protein-1 (MCP-1) level was significantly decreased on the 14th day after the administration of TPs (Ponist et al, 2020).…”

Section: Anti-inflammationmentioning

confidence: 99%

Molecular mechanisms and applications of tea polyphenols: A narrative review

et al. 2021

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Tea not only has a beloved taste, but also has rich biological activity, so it has become one of the best-selling beverages in the world. In the existing studies on tea, it is found that TPs account for highest proportion, which is the main bioactive ingredient. Generally, green tea polyphenols (GTP S ) are the main research materials, which are high in catechins. GTP S have significant health benefits (Xing et al., 2019) and are considered to be safe by the US Food and Drug Administration (Chang et al., 2019). Since the 1950s, scholars have begun to study the biological activity of TPs and their mechanism of action, and found that TPs are not only beneficial to human health, but also have an impact on food, medical, chemical and other fields.According to the research on the components of TPs (Khan & Mukhtar, 2018;Li et al., 2019), it is found that TPs contain catechins, flavonoids, anthocyanins, phenolic acids and other substances, among which catechins account for the largest proportion, up to 60%-80%. Catechin is divided into epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC) and epigallocatechin gallate (EGCG; the molecular structure is shown in the Figure 1). The content relationship is EGCG > EGC > ECG > EC, of which EGCG accounts for about 70%. Therefore, EGCG is the main catechin and the research on the biological activity of TPs mainly starts from EGCG.The research conclude that TPs can exert a variety of biological activities such as anti-oxidation, anti-tumor, anti-inflammation,

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Modulation of methotrexate efficacy by green tea polyphenols in rat adjuvant arthritis

Cited by 9 publications

References 45 publications

Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis

Bioflavonoid Robinin from Astragalus falcatus Lam. Mildly Improves the Effect of Metothrexate in Rats with Adjuvant Arthritis

Rhodiola rosea L. Extract, a Known Adaptogen, Evaluated in Experimental Arthritis

Molecular mechanisms and applications of tea polyphenols: A narrative review

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