Modulation of microRNA 20b with resveratrol and longevinex is linked with their potent anti‐angiogenic action in the ischaemic myocardium and synergestic effects of resveratrol and γ‐tocotrienol

Mukhopadhyay, Partha; Das, Somak Kumar; Ahsan, Kaimul; Otani, Hajime; Das, Dipak K.

doi:10.1111/j.1582-4934.2011.01480.x

Cited by 35 publications

(51 citation statements)

References 40 publications

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“…Here we revealed the role of miR-20b in the down-regulation of VEGF, which mediates vascular permeability and angiogenesis in HRECs. Using miR-20b mimic transfection, we further demonstrated that HG-induced VEGF up-regulation and subsequent functional changes, i.e., vascular permeability and angiogenesis, can be prevented by increasing the level of miR-20b in HRECs, which is consistent with the previous study showing that miR-20b has an antiangiogenic effect in the ischemic myocardium [27]. Furthermore, by using bioinformatics analytic tool and luciferase reporter assay, we identified AKT3 as a target of miR-20b.…”

Section: Discussionsupporting

confidence: 79%

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

Qin

Liu

et al. 2016

ABBS

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Diabetic retinopathy (DR) is the leading cause of new-onset blindness. The roles of microRNAs in diabetic retinopathy are largely unknown. The aim of this study is to investigate the role of miR20b in DR. Transfection of miR-20b mimic in high glucose (HG)-treated human retinal endothelial cells (HRECs) increased miR-20b expression and decreased the expression level of VEGF mRNA, while transfection of miR-20b inhibitor in control HRECs reduced the miR-20b expression with a corresponding increase of VEGF mRNA. In vitro functional assay showed that transfection of miR-20b mimic prevented HG-induced increase in transendothelial permeability and tube formation in HRECs. Transfection of miR-20b inhibitor or treatment of VEGF increased transendothelial permeability and tube formation in control HRECs. Luciferase reported assay showed that AKT3 is a target of miR-20b. Transfection of miR-20b mimic prevented the up-regulation of AKT3 induced by HG without changing the protein levels of other isoforms of AKT, and silencing of AKT3 caused decrease of VEGF mRNA and protein levels as well as prevented HG-induced increase in transendothelial permeability and tube formation. Finally, we showed that miR-20b was down-regulated in the retina and retinal endothelial cells in diabetic rats, with a correlated up-regulation of VEGF and AKT3. Intravitreal injection of miR-20b mimic in the diabetic rat significantly increased the miR-20b expression and decreased the expression levels of AKT3 and VEGF in the retina tissues, and intravitreal delivery of AKT3 siRNA in the diabetic rat significantly decreased the expressions of AKT3 and VEGF. Collectively, miR-20b is important for the regulation of VEGF-mediated changes in HRECs and rat retinal tissues under hyperglycemic conditions possibly via targeting AKT3.

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Section: Discussionsupporting

confidence: 79%

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

Qin

Liu

et al. 2016

ABBS

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“…In 2009, Wang and colleagues reported that MiR-327 expression levels were up-regulated in type-2 diabetic rat models using a miRNA microarray [21]. Subsequently, studies have shown that miR-327 increased by 10-fold in rats with MIRI and nearly 3-fold in the kidneys of rats with renal I/R injury, respectively [22,23]. Additionally, in injured sciatic nerves, miR-327 was increased and involved in peripheral nerve regeneration by targeting CCL2 in a 3′-UTR-dependent manner [38].…”

Section: Discussionmentioning

confidence: 99%

“…I/R heart tissues and renal I/R injury models [21][22][23]. A search using the public prediction algorithm TargetScan (http://www.targetscan.org/) shows that RP105 is a possible target gene of miR-327.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

Down-Regulation of miR-327 Alleviates Ischemia/Reperfusion-Induced Myocardial Damage by Targeting RP105

Yang

Liu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Micro RNAs (miRNAs) play a very important role in myocardial ischemia/ reperfusion injury (MIRI), including in inflammation, apoptosis, and angiogenesis. Previous studies have demonstrated up-regulation of miR-327 in renal ischemia/reperfusion injury and MIRI. Via TargetScan, we found RP105 is a possible target gene of miR-327; our previous studies have also confirmed that RP105 acted as a cardioprotective protein in MIRI by reducing inflammation. However, the regulatory effect of miR-327 on RP105 has not previously been proposed. In our study, we aimed to identify the regulatory effect of miR-327 on RP105 protein in MIRI rats. Methods: Sixty male Sprague–Dawley rats were randomly divided into five groups, which were pre-treated with saline (sham and ischemia/reperfusion group), adenovirus-expressing miR-327-RNAi (Ad-miR-327-i group), control (Ad-NC group), or pri-miR-327 (Ad-miR-327 group) treatments. Three days later, the rat MIRI model was established by ischemia for 30 min, followed by reperfusion for 3 h. Myocardium and plasma were harvested and assessed. Results: miR-327 was increased by nearly 3-fold both in myocardium and plasma, which down-regulated RP105 in a 3′-untranslated region-dependent manner, and down-regulation of miR-327 via adenovirus transfection indirectly suppressed the TLR4/ TLR2-MyD88-NF-κB signaling axis activation via up-regulation of RP105, which subsequently resulted in reduced myocardial infarct size, attenuated cardiomyocyte destruction, and alleviated inflammation. In contrast, up-regulation of miR-327 induced the opposite effect. Conclusion: Down-regulation of miR-327 exerts a cardioprotective effect against MIRI by reducing inflammation, which may constitute a promising molecular therapeutic target for treating MIRI.

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“…The upregulated miRNAs included miR-20b and miR-21 (antiangiogenic), which are implicated in cardiac remodeling. These data suggest that resveratrol exerts a significant cardioprotection through miRNA modulation [69,70]. …”

Section: Modulation Of Mirna Expression By Natural Agentsmentioning

confidence: 99%

MicroRNAs as targets for dietary and pharmacological inhibitors of mutagenesis and carcinogenesis

Izzotti

Cartiglia

Steele

et al. 2012

Mutation Research/Reviews in Mutation Research

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MicroRNAs (miRNAs) have been implicated in many biological processes, cancer, and other diseases. In addition, miRNAs are dysregulated following exposure to toxic and genotoxic agents. Here we review studies evaluating modulation of miRNAs by dietary and pharmacological agents, which could potentially be exploited for inhibition of mutagenesis and carcinogenesis. This review covers natural agents, including vitamins, oligoelements, polyphenols, isoflavones, indoles, isothiocyanates, phospholipids, saponins, anthraquinones and polyunsaturated fatty acids, and synthetic agents, including thiols, nuclear receptor agonists, histone deacetylase inhibitors, antiinflammatory drugs, and selective estrogen receptor modulators. As many as 145 miRNAs, involved in the control of a variety of carcinogenesis mechanisms, were modulated by these agents, either individually or in combination. Most studies used cancer cells in vitro with the goal of modifying their phenotype by changing miRNA expression profiles. In vivo studies evaluated regulation of miRNAs by chemopreventive agents in organs of mice and rats, either untreated or exposed to carcinogens, with the objective of evaluating their safety and efficacy. The tissue specificity of miRNAs could be exploited for the chemoprevention of site-specific cancers, and the study of polymorphic miRNAs is expected to predict the individual response to chemopreventive agents as a tool for developing new prevention strategies.

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Modulation of microRNA 20b with resveratrol and longevinex is linked with their potent anti‐angiogenic action in the ischaemic myocardium and synergestic effects of resveratrol and γ‐tocotrienol

Cited by 35 publications

References 40 publications

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy

Down-Regulation of miR-327 Alleviates Ischemia/Reperfusion-Induced Myocardial Damage by Targeting RP105

MicroRNAs as targets for dietary and pharmacological inhibitors of mutagenesis and carcinogenesis

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