Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

Kolhe, Ravindra; Mondal, Ashis; Pundkar, Chetan; Periyasamy‐Thandavan, Sudharsan; Mendhe, Bharati; Hunter, Monte; Isales, Carlos M.; Hill, William; Hamrick, Mark W.; Fulzele, Sadanand

doi:10.3390/nu10020186

Cited by 19 publications

(14 citation statements)

References 67 publications

(94 reference statements)

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“…There is evidence that daily supplementation with 1000 mg/day of vitamin C for six weeks can improve blood glucose, lipid profiles, glycated haemoglobin (HbA1C) and insulin in subjects with T2DM ( Afkhami-Ardekani & Shojaoddiny-Ardekani, 2007 ). The effect of vitamin C on subjects can be observed at the gene expression level and mechanistically may involve epigenetic regulation including via microRNAs (miRNAs or miRs) ( Kolhe et al, 2018 ). Supporting this model, Kim et al (2015) revealed dietary consumption of high dose vitamin C enhances anti-oxidation and anti-glycation as well as reduction of inflammation-related miRNAs in lipoproteins.…”

Section: Introductionmentioning

confidence: 99%

“…Supporting this model, Kim et al (2015) revealed dietary consumption of high dose vitamin C enhances anti-oxidation and anti-glycation as well as reduction of inflammation-related miRNAs in lipoproteins. Furthermore, treatment of human bone marrow stem cells with vitamin C has been shown to modulate miRNA expression in these cells ( Kolhe et al, 2018 ). These studies strongly suggest that vitamin C can affect miRNA expression and may be involved in the pathophysiology of T2DM.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus and high oxidative stress

Ruknarong

Boonthongkaew

Chuangchot

et al. 2021

PeerJ

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Background Vitamin C is an essential element required for normal metabolic function. We investigated the effect of vitamin C supplementation on circulating miRNA (miR) expression in subjects with poorly controlled type 2 diabetes mellitus (T2DM). Changes in miR expression were also correlated with clinical measures of disease. Methods Pre- and post-vitamin C supplementation samples from five participants who had increased vitamin C levels, improved oxidative status and polymorphonuclear (PMN) function after receiving 1,000 mg of vitamin C daily for six weeks were screened for miRNA expression using the NanoString miRNA assay. Differences in miRNA expression identified from the miRNA screen were validated by qRT-PCR. Results Four miRNAs showed significantly different expression post-vitamin C supplementation relative to baseline, including the down-regulation of miR-451a (−1.72 fold change (FC), p = 0.036) and up-regulation of miR-1253 (0.62 FC, p = 0.027), miR-1290 (0.53 FC, p = 0.036) and miR-644a (0.5 FC, p = 0.042). The validation study showed only miR-451a expression was significantly different from baseline with vitamin C supplementation. MiR-451a expression was negatively correlated with vitamin C levels (r = − 0.497, p = 0.049) but positively correlated with levels of malondialdehyde (MDA) (r = 0.584, p = 0.017), cholesterol (r = 0.564, p = 0.022) and low-density lipoproteins (LDL) (r = 0.522, p = 0.037). Bioinformatics analysis of the putative target genes of miR-451a indicated gene functions related to signaling pathways involved in cellular processes, such as the mammalian target of rapamycin (mTOR) signaling pathway. Conclusions Vitamin C supplementation altered circulating miR-451a expression. The results from this pilot study suggest that miRNAs could be used as biomarkers to indicate oxidative status in subjects with T2DM and with poor glycemic control and could lead to a novel molecular strategy to reduce oxidative stress in T2DM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus and high oxidative stress

Ruknarong

Boonthongkaew

Chuangchot

et al. 2021

PeerJ

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“…miRNAs play vital roles in the absorption of vitamin C in the intestines by modulating the post-transcriptional regulation of several vitamin transporter genes, including that of solute carrier family ( SLC)23A2 , SLC19A2 , SLC52A3 , SLC26A3 and SLC15A1 ( 31 ). Vitamin C can also regulate multiple stem cell-specific signaling pathways, such as cell adhesion molecules, fatty acid biosynthesis and hormone signaling pathways by altering miRNA expression ( 32 ).…”

Section: Introductionmentioning

confidence: 99%

Modulation of miRNAs by vitamin C in H2O2‑exposed human umbilical vein endothelial cells

Liang

et al. 2020

Int J Mol Med

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Vitamin C plays a protective role in oxidative damage by blocking the effects of free radicals. The present study investigated the mechanisms through which vitamin C partly mediates anti-apoptotic and antioxidant functions via the regulation of microRNAs (miRNAs or miRs). For this purpose, a global miRNA expression analysis on human umbilical vein endothelial cells (HUVECs) treated with vitamin C was conducted using microarrays containing human precursor and mature miRNA probes. The results revealed that there were 42 identical miRNAs among the differentially expressed miRNAs in the HUVEC group and H 2 O 2 + vitamin C-treated HUVEC group compared to the H 2 O 2 -exposed HUVEC group, including 41 upregulated miRNAs and 1 down-regulated miRNA. Using bioinformatics analysis, differentially expressed miRNAs were investigated to identify novel target mRNAs and signaling pathways. Pathway enrichment analyses revealed that apoptosis, the mitogen-activated protein kinase (MAPK) signaling pathway, phosphoinositide 3-kinase (PI3K)/Akt signaling pathway and oxidative phosphorylation were significantly enriched. The results from western blot analysis demonstrated that the interleukin (IL)10, matrix metalloproteinase (MMP)2, cAMP-response element binding protein (CREB) and p-CREB protein expression levels in HUVECs transfected with hsa-miR-3928-5p and induced by H 2 O 2 were significantly downregulated; the MAPK9, caspase-3 (CASP3) and p-CASP3 protein expression levels in HUVECs transfected with hsa-miR-323a-5p and induced by H 2 O 2 were significantly downregulated. The present study therefore demonstrates that vitamin C partly exerts protective effects on HUVECs through the regulation of miRNA/mRNA axis expression.

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“…Kolhe et al tried to verify the hypothesis that vitamin C mediates proliferation and differentiation of bone marrow stromal cells through miRNA regulation [ 35 ]. They performed bioinformatics analyses to identify novel target genes and signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

Artificial Intelligence in Nutrients Science Research: A Review

Sak

Suchodolska

2021

Nutrients

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Artificial intelligence (AI) as a branch of computer science, the purpose of which is to imitate thought processes, learning abilities and knowledge management, finds more and more applications in experimental and clinical medicine. In recent decades, there has been an expansion of AI applications in biomedical sciences. The possibilities of artificial intelligence in the field of medical diagnostics, risk prediction and support of therapeutic techniques are growing rapidly. The aim of the article is to analyze the current use of AI in nutrients science research. The literature review was conducted in PubMed. A total of 399 records published between 1987 and 2020 were obtained, of which, after analyzing the titles and abstracts, 261 were rejected. In the next stages, the remaining records were analyzed using the full-text versions and, finally, 55 papers were selected. These papers were divided into three areas: AI in biomedical nutrients research (20 studies), AI in clinical nutrients research (22 studies) and AI in nutritional epidemiology (13 studies). It was found that the artificial neural network (ANN) methodology was dominant in the group of research on food composition study and production of nutrients. However, machine learning (ML) algorithms were widely used in studies on the influence of nutrients on the functioning of the human body in health and disease and in studies on the gut microbiota. Deep learning (DL) algorithms prevailed in a group of research works on clinical nutrients intake. The development of dietary systems using AI technology may lead to the creation of a global network that will be able to both actively support and monitor the personalized supply of nutrients.

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Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells

Cited by 19 publications

References 67 publications

Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus and high oxidative stress

Vitamin C supplementation reduces expression of circulating miR-451a in subjects with poorly controlled type 2 diabetes mellitus and high oxidative stress

Modulation of miRNAs by vitamin C in H2O2‑exposed human umbilical vein endothelial cells

Artificial Intelligence in Nutrients Science Research: A Review

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