Modulation of monocyte matrix metalloproteinase-2 by breast adenocarcinoma cells

Szabo, Kristina; Singh, Gurmit

doi:10.1186/bcr1261

Cited by 10 publications

(9 citation statements)

References 47 publications

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“…Gelatin zymography revealed a low level of MMP-2 secretion in conditioned media from MDA-MB-231 cells. This is consistent with previous studies reporting basal MMP-2 expression in cultured MDA-MB-231 cells ranging from undetectable to very low levels [23][24][25], as well as the notion that epithelial carcinomas, such as breast cancer, typically produce minimal amounts of MMP-2 [26,27]. The low level of MMP-2 secretion from MDA-MB-231, along with the evidence that inhibition of MMP-2 in these cells did not alter their ability to migrate across an artificial basement membrane, argues against the predominant role of malignant cell-derived MMP-2 in matrix degradation during cancer invasion or extravasation under the culture condition.…”

Section: Discussionsupporting

confidence: 94%

A role for endothelial-derived matrix metalloproteinase-2 in breast cancer cell transmigration across the endothelial-basement membrane barrier

Kargozaran

Yuan

Breslin

et al. 2007

Clin Exp Metastasis

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Invasive cancer cells utilize matrix metalloproteinases (MMPs) to degrade the extracellular matrix and basement membrane in the process of metastasis. Among multiple members of the MMP family, the gelatinase MMP-2 has been implicated in the development and dissemination of malignancies. However, the cellular source of MMP-2 and its effect on metastatic extravasation have not been well characterized. The objective of this study was to test the hypothesis that active MMP-2 derived from endothelial cells facilitated the transmigration of breast cancer cells across the microvascular barrier. Gelatin zymography was used to assess latent and active MMP-2 production in conditioned media from MDA-MB-231 human breast cancer cells, human lung microvascular endothelial cells (HLMVEC) and co-culture of these two cells. Transmigrated cancer cells were measured during MMP-2 knockdown with siRNA and pharmacological inhibition of MMP activity with OA-HY. The results showed consistent MMP-2 secretion by the HLMVECs, whereas a low level production was seen in the MDA-MB-231 cells. Inhibition of MMP-2 expression or activity in HLMVECs significantly attenuated the transmigration of MDA-MB-231 cells across an endothelial monolayer barrier grown on a reconstituted basement membrane. The data provide evidence supporting a potential role for the endothelial production of MMPs in promoting cancer cell extravasation. We suggest that the interaction between malignant cells and peritumoral benign tissues including the vascular endothelium may serve as an important mechanism in the regulation of tumor invasion and metastasis.

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Section: Discussionsupporting

confidence: 94%

A role for endothelial-derived matrix metalloproteinase-2 in breast cancer cell transmigration across the endothelial-basement membrane barrier

Kargozaran

Yuan

Breslin

et al. 2007

Clin Exp Metastasis

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“…We observed increases in secretion of MMP-2 and -9 from U937 cells that had been incubated with 231-CM. Similar results have been reported for other populations of macrophages, e.g., macrophages differentiated from human peripheral blood monocytes [14] and the human THP-1 monocytic cell line [41] and linked to increased invasiveness of a variety of breast carcinoma cell lines. Thus, as with CTSB, increases in TAM expression of MMP-2 and -9 are induced by interactions between carcinoma cells and macrophages within the tumor microenvironment.…”

Section: Bsupporting

confidence: 73%

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

Mohamed¹,

Rudy²,

Anbalagan³

et al. 2010

Cell Physiol Biochem

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In the tumor microenvironment, monocytes respond to paracrine stimuli from breast cancer cells by secreting molecules that participate in breast cancer growth, invasion, intravasation and metastasis. Here we examined the effects of media conditioned by MDA-MB-231 human breast carcinoma cells (231-CM) on expression and secretion of proteases and secretion of cytokines by U937 human monocytes. We found that 231-CM increased U937: 1) proliferation; 2) expression, activity and secretion of the cysteine protease cathepsin B (CTSB); 3) secretion of matrix metalloproteinases (MMP)-2 and -9; and 4) secretion of interleukin-6 (IL-6) and insulinlike growth factor binding protein-1 (IGFBP-1). We further demonstrated by western blotting and enzymatic activity assays that the increases in CTSB secretion and activity induced by 231-CM could be reduced by neutralizing antibodies against IL-6. Our data suggest a role for IL-6 in

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“…23,35 This finding in prior studies is supported by the findings in the present one, in which tumor cells did not show MMP-2 expression either by in situ hybridization or by immunostaining.…”

Section: Discussionsupporting

confidence: 88%

Matrix Metalloproteinase-2 Is Expressed in Melanoma-Associated Spongiform Scleropathy

Alyahya

Kolko

Prause

et al. 2008

Invest. Ophthalmol. Vis. Sci.

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MASS is considered a tumor-induced scleral degradation process. There is a significantly higher expression of MMP-2 in MASS-positive areas, indicating that MMP-2 is involved in the development of MASS and that MMP-2 is produced by scleral fibroblasts under the influence of tumor cells and/or tumor-infiltrating macrophages. These changes may represent a step in the invasion of uveal melanoma by facilitating the spread of tumor cells through the sclera.

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Modulation of monocyte matrix metalloproteinase-2 by breast adenocarcinoma cells

Abstract: Introduction The presence of monocyte and macrophage cells in growing breast tumors, and the positive relationship between the degree of immune cell infiltration and tumor growth, suggest a possible paracrine growth regulatory function of immune cells in breast cancer.

Cited by 10 publications

References 47 publications

A role for endothelial-derived matrix metalloproteinase-2 in breast cancer cell transmigration across the endothelial-basement membrane barrier

A role for endothelial-derived matrix metalloproteinase-2 in breast cancer cell transmigration across the endothelial-basement membrane barrier

Interleukin-6 Increases Expression and Secretion of Cathepsin B by Breast Tumor-Associated Monocytes

Matrix Metalloproteinase-2 Is Expressed in Melanoma-Associated Spongiform Scleropathy

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