Modulation of N-methyl-N-nitrosourea induced mammary tumors in Sprague–Dawley rats by combination of lysine, proline, arginine, ascorbic acid and green tea extract

Roomi, M. Waheed; Roomi, Nusrath; Ivanov, Vadim; Kalinovsky, Tatiana; Niedzwiecki, Aleksandra; Rath, Matthias

doi:10.1186/bcr989

Cited by 60 publications

(47 citation statements)

References 12 publications

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“…Many researchers demonstrated induction of mammary tumours in rats through the use of chemical carcinogens such as Nmethylnitrosurea, dimethylhydrazine and 7, 12-dimethylbenz (a) anthracene [5][6][7]. Other chemical inducers of tumours include the administration of high doses of growth hormones, oestrogens or aminofluorene compounds that stimulate the development of mammary tumours [8].…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of MCF7 mammary carcinoma xenografts in a non-immunocompromised rat model

Dunpall¹,

Opoku²,

Revaprasadu³

2016

Trop. J. Pharm Res

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Section: Introductionmentioning

confidence: 99%

Development and characterization of MCF7 mammary carcinoma xenografts in a non-immunocompromised rat model

Dunpall¹,

Opoku²,

Revaprasadu³

2016

Trop. J. Pharm Res

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“…Our previous MNU study (12), based on previous investigations, was conducted for as extensive a period as 30 weeks following the MNU injection. Various studies by Thompson and associates (6,8,16) indicated that the prolonged period of experimentation of such studies can be considerably reduced when MNU is administered to 21-day-old sexually immature female rats rather than to 50-day-old female rats.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we conducted the proposed study by injecting MNU into the rats at 21 days of age. In our previous MNU study, the animals were exposed to treatments for an extensive period prior to the tumors reaching a palpable size (12 Other reagents used were of the the highest purity and were obtained from Sigma Aldrich (Mumbai, India).…”

Section: Introductionmentioning

confidence: 99%

“…N-methyl-N-nitrosourea (MNU)-induced mammary carcinogenesis in female rats is frequently used as an animal model for the investigation of breast carcinogenesis and the treatment of breast cancer in humans (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In our previous studies with MNU-induced rat mammary carcinogenesis, we observed that the specific formulation of nutrient supplements containing ascorbic acid, L-lysine, L-proline, L-arginine, N-acetyl cysteine selenium, copper and manganese along with green tea extract (GTE) fed to the animals reduced the incidence, number and weights of the tumors (12) measured 30 weeks post-MNU.…”

Section: Introductionmentioning

confidence: 99%

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A combination of green tea extract, specific nutrient mixture and quercetin: An effective intervention treatment for the regression of N-methyl-N-nitrosourea (MNU)-induced mammary tumors in Wistar rats

Kale

Gawande²,

Kotwal³

et al. 2010

Oncology Letters

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Abstract. Mammary tumors were developed by intraperitoneal injection of N-methyl-N-nitrosourea (MNU) in 21-day-old, sexually immature female Wistar rats. Injection of MNU was repeated 14 weeks after the first one. When palpable tumors were evident in all of the rats, various dietary treatments were initiated for a period of 8 weeks. The treatments were designed to provide 30 mg green tea extract either alone or as a nutrient mixture (E). E was then expanded to include either a nutrient supplement (N), quercetin (Q) or both (N+Q). At the end of the treatment, tumor size/rat measured in the live rats was significantly lower in the groups receiving E, E+Q, E+N and E+N+Q than in the positive control (PC) group which did not receive any dietary treatment. Tumor number/ rat, tumor volume/rat and tumor weight/rat were evaluated after sacrificing the rats on the 60th day. The rats receiving E+N+Q showed significantly lower values for the three parameters as compared to the PC group. The PC group showed 24 carcinomas mostly of grade III severity, while the E+N+Q group had only 6 carcinomas, all of which were of grade II severity. IntroductionN-methyl-N-nitrosourea (MNU)-induced mammary carcinogenesis in female rats is frequently used as an animal model for the investigation of breast carcinogenesis and the treatment of breast cancer in humans (1-12). In our previous studies with MNU-induced rat mammary carcinogenesis, we observed that the specific formulation of nutrient supplements containing ascorbic acid, L-lysine, L-proline, L-arginine, N-acetyl cysteine selenium, copper and manganese along with green tea extract (GTE) fed to the animals reduced the incidence, number and weights of the tumors (12) measured 30 weeks post-MNU. The combination was designated by the authors as 'Nutrient Synergy' (NS) to underscore the synergistic action of various constituents. The beneficial results obtained in the studies were mainly attributed to epigallocatechin-3-gallate (EGCG), the principal anticancer agent present in the GTE in the NS (13,14). Our previous in vitro studies with cancer cell lines demonstrated that an increase in the concentration of EGCG in the cell culture media resulted in increased anticancer activity. These findings suggested that when the plasma levels of EGCG are increased, this increase is reflected in the elevated anticancer activity. Our recent investigation (15) showed that the plasma level of EGCG in rats was elevated by as much as 25% when a small amount of quercetin (Q) was administered along with GTE as a nutrient mixture (E) + nutrient supplement (N). It was therefore hypothesized that the anticancer activity of E is enhanced when Q is fed in combination with E+N.Our previous MNU study (12), based on previous investigations, was conducted for as extensive a period as 30 weeks following the MNU injection. Various studies by Thompson and associates (6,8,16) indicated that the prolonged period of experimentation of such studies can be considerably reduced when MNU is administered to 21-day-old sexually...

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“…These nutrients may exert their antitumor effects via inhibition of MMPs and strengthening the connective tissue surrounding cancer cells. Additionally, it has been suggested that, through inhibition of hyaluronidase, ascorbic acid can prevent metastases by preventing degradation of the ground substance surrounding the tumor [12]. Different mechanisms are involved in the metastatic cascade including angiogenesis, cellular adhesion, local proteolysis, and tumor cell migration [9,13].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of tumor tissue in CT-26 implanted BALB/C mouse after treatment with ascorbic acid

Lee¹,

Lee²,

Park³

et al. 2012

Cellular and Molecular Biology Letters

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Abbreviations used: ATP -adenosine triphosphate; DTT -dithiothreitol; ECMextracellular matrix; GDT -guanosine diphosphate; LR -log ratio; MALDI TOF-MS/MS -Matrix-assisted laser-desorption ionization time-of-flight tandem mass spectroscopy; MMPs -matrix metalloproteinases; PAGE -polyacrylamide gel electrophoresis; PBSphosphate buffered solution; PCR -polymerase chain reaction; SDS -sodium dodecyl sulfate; TCA -trichloroacetic acid; TCTP -translationally controlled tumor protein; 2-DE -two-dimensional gel electrophoresis Abstract: Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression and protein modification. Proteome changes of tumor tissue were investigated after intraperitoneal administration of a high concentration of ascorbic acid in BALB/C mice implanted with CT-26 cancer cells using two-dimensional gel electrophoresis and mass spectrometry. Eighteen protein spots were identified whose expression was different between control and ascorbic acid treatment groups. In particular, eukaryotic translation initiation factor 3 subunit 1, nucleophosmin, latexin, actin-related protein 2/3 complex subunit 5, M2-type pyruvate kinase, vimentin, tumor protein translationally-controlled 1, RAS oncogene family Ran, plastin 3 precursor, ATPase, Rho GDT dissociation inhibitor β, and proteasome activator subunit 2 expression were quantitatively up-regulated. The increase in the level of these proteins was accompanied by an increase in mRNA level. The cytoskeleton protein actin, vimentin, and tumor protein translationally-controlled 1 showed quantitative expression profile differences. A change in actin cytoskeleton distribution, functionally relevant to the proteome result, was observed after treatment with ascorbic acid. These

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Modulation of N-methyl-N-nitrosourea induced mammary tumors in Sprague–Dawley rats by combination of lysine, proline, arginine, ascorbic acid and green tea extract

Cited by 60 publications

References 12 publications

Development and characterization of MCF7 mammary carcinoma xenografts in a non-immunocompromised rat model

Development and characterization of MCF7 mammary carcinoma xenografts in a non-immunocompromised rat model

A combination of green tea extract, specific nutrient mixture and quercetin: An effective intervention treatment for the regression of N-methyl-N-nitrosourea (MNU)-induced mammary tumors in Wistar rats

Proteomic analysis of tumor tissue in CT-26 implanted BALB/C mouse after treatment with ascorbic acid

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