Modulation of nephrotoxicity induced by gentamicin with bone marrow mesenchymal stem cells and Moringa oleifera extract

Ismaiel, Elsaid Am; El-Latif, Hekma A. Abd; Mohammed, Hagar E; Fattah, May A. Abd El; Rashed, Laila; Abdel-Wahhab, Nossad Attia

doi:10.21608/ejchem.2019.18347.2129

Cited by 2 publications

(2 citation statements)

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“…The findings of this study suggest that hyperlipidemia, oxidative stress, and inflammation resulting from GM injection may all be factors that contribute to the kidney's alteration, promoting the development of nephrotoxicity [8,59,63]. Consequently, there is much interest in using plants rich in phenols and flavonoids because of their hypolipidemic characteristics, capacity to scavenge free radicals and antioxidant and anti-inflammatory properties.…”

Section: Discussionmentioning

confidence: 89%

Ethanolic Extracts of Grape and Guava Seeds Ameliorate Gentamicin Induced Acute Renal Injury in Rats

et al. 2022

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Objective: This investigation was designed to analyze the phytochemicals of the ethanolic extracts of grape and guava seeds and to assess their possible ameliorating effects on gentamicin (GM)-induced nephropathy in rats. Material and methods: Sixty-four male Sprague Dawley rats were randomly allocated into eight groups and treated for ten consecutive days as follows: Group 1 (control group) was injected intraperitoneally with saline solution; Groups 2, 3, and 4 received a daily oral dosage of grape seed extract (GrSE, 40 mg/kg b.wt /day, p.o), guava seed extracts (GuSE, 300 mg/ b.wt /day, p.o) or a combination of both, respectively. Group 5 was intoxicated with GM (100 mg/kg/ b.wt /day, i.p). Groups 6, 7, and 8 received an oral dose of GrSE and/or GuSE, along with an intraperitoneal injection of GM. Results: Chemical analysis by high-performance liquid chromatography with diode-array detection (HPLC-DAD) showed that the ethanolic extracts of GrSE and GuSE are rich in phenolic compounds such as gallic acid, chlorogenic acid, 3-hydroxycinnamic acid, ferulic acid, caffeic acid, ellagic acid, daidzein, apigenin, syringic acid, naringenin, p-coumaric acid, quercetin, rutin, and vanillic acid. GrSE or GuSE oral administration did not cause any considerable biochemical or histological alterations in the normal rats. Rats treated with GM showed significant elevation in serum concentration of urea and creatinine and a significant reduction in serum levels of total proteins, sodium, and potassium. In contrast, serum alkaline phosphatase activity was significantly elevated. Additionally, GM injection resulted in a significant rise in the serum levels of triacylglycerol (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL), and a significant decrement in serum high-density lipoprotein cholesterol (HDL). Furthermore, GM-injected rats displayed a substantial decline in the activity of erythrocyte copper, zinc superoxide dismutase (Cu, Zn-SOD), and blood and renal levels of reduced glutathione (GSH) along with elevation in the renal level of malondialdehyde (MDA). Upregulation of kidney injury molecule-1 (KIM-1) and nuclear factor kappa B p65 subunit (NF-κB p65) gene expression was also observed in GM-injected rats compared with control. Simultaneous administration of GrSE and/or GuSE with gentamicin attenuated the nephrotoxic effects of GM as indicated by improvement in renal function parameters, normalization of serum electrolytes, restoration of serum lipid profile, reduction in renal lipid peroxidation, enhancement of the antioxidant status and down-regulation of KIM-1 and NF-κB p65 gene expression. The results of the histopathological evaluation confirmed these biochemical findings. Conclusion: Treatment with grape and guava seed extracts ameliorated gentamicin-induced nephrotoxicity via hypolipidemic, antioxidant, and anti-inflammatory effects.

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Section: Discussionmentioning

confidence: 89%

Ethanolic Extracts of Grape and Guava Seeds Ameliorate Gentamicin Induced Acute Renal Injury in Rats

et al. 2022

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“…The high concentration of stem/progenitor hematopoietic cells in UCB makes it useful for treating hematopoietic and genetic disorders ( 23 ). The use of stem cells to treat complicated conditions like AKI has received increased attention ( 24 ). It has been demonstrated that mesenchymal stem cells (MSCs) may help treat AKI ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats

et al. 2021

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Background: Acute kidney injury (AKI), also known as acute renal failure (ARF), has received considerable critical attention in recent years. Gentamicin (GM) is an antibiotic whose prolonged use results in AKI with a high mortality rate.Methods: Fifty adult female albino rats weighing 150–200 g were used. The animals were divided into five groups: the first group was the normal healthy control one, the second group received only 1 × 106 HUCB mononuclear cells (MNCs)/rat by intravenous (iv) injection, the third diseased group was given GM 100 mg/kg for 10 consecutive days by intraperitoneal injections, the fourth preventive group received 1 × 106 HUCB MNCs/rat by iv injection 24 h before gentamicin treatment, and the fifth treated group received 1 × 106 HUCB MNCs/rat by iv injection 24 h after gentamicin treatment. After 1 week of treatment, blood samples were collected, and kidneys were removed for histopathological examination.Results: Rats treated with HUCB MNCs in the treated group had a significant decrease in renal damage, low levels of biomarkers for nephrotoxicities such as serum creatinine and blood urea nitrogen, and low chromosomal aberrations compared to the diseased third group. The gene expression of KIM-1 and NGAL was decreased in response to HUCB treatment.Conclusions: HUCB MNCs have a curative effect against AKI and gentamicin-induced genotoxicity owing to their regenerative property.

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Modulation of nephrotoxicity induced by gentamicin with bone marrow mesenchymal stem cells and Moringa oleifera extract

Cited by 2 publications

References 57 publications

Ethanolic Extracts of Grape and Guava Seeds Ameliorate Gentamicin Induced Acute Renal Injury in Rats

Ethanolic Extracts of Grape and Guava Seeds Ameliorate Gentamicin Induced Acute Renal Injury in Rats

Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats

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