Modulation of Rat Kidney Stone Crystallization and the Relative Oxidative Stress Pathway by Green Tea Polyphenol

Li, Zizhi; Chang, Linna; Ren, Xiuli; Hu, Yanan; Chen, Zhenhua

doi:10.1021/acsomega.0c05903

Cited by 22 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to growing evidence, oxidative stress may play an essential role in hyperoxaluria-induced kidney injury, resulting in renal CaOx crystallization ( 28 – 30 ). Herein, we examined the potential antioxidative properties of BSHS in this animal model.…”

Section: Resultsmentioning

confidence: 99%

Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Bushen Huashi decoction against kidney stones

Liu

Cao²,

Jin³

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

IntroductionKidney stone disease (KS) is a complicated disease with an increasing global incidence. It was shown that Bushen Huashi decoction (BSHS) is a classic Chinese medicine formula that has therapeutic benefits for patients with KS. However, its pharmacological profile and mechanism of action are yet to be elucidated.MethodsThe present study used a network pharmacology approach to characterize the mechanism by which BSHS affects KS. Compounds were retrieved from corresponding databases, and active compounds were selected based on their oral bioavailability (≥30) and drug-likeness index (≥0.18). BSHS potential proteins were obtained from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database, whereas KS potential genes were obtained from GeneCards and OMIM, TTD, and DisGeNET. Gene ontology and pathway enrichment analysis were used to determine potential pathways associated with genes. The ingredients of BSHS extract were identified by the ultra‐high‐performance liquid chromatography coupled with quadrupole orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS). The network pharmacology analyses predicted the potential underlying action mechanisms of BSHS on KS, which were further validated experimentally in the rat model of calcium oxalate kidney stones.ResultsOur study found that BSHS reduced renal crystal deposition and improved renal function in ethylene glycol(EG)+ammonium chloride(AC)-induced rats, and also reversed oxidative stress levels and inhibited renal tubular epithelial cell apoptosis in rats. BSHS upregulated protein and mRNA expression of E2, ESR1, ESR2, BCL2, NRF2, and HO-1 in EG+AC-induced rat kidney while downregulating BAX protein and mRNA expression, consistent with the network pharmacology results.DiscussionThis study provides evidence that BSHS plays a critical role in anti-KS via regulation of E2/ESR1/2, NRF2/HO-1, and BCL2/BAX signaling pathways, indicating that BSHS is a candidate herbal drug for further investigation in treating KS.

show abstract

Section: Resultsmentioning

confidence: 99%

Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Bushen Huashi decoction against kidney stones

Liu

Cao²,

Jin³

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“… 43 , 44 During crystallization, succinic, salicyluric acid and gentisic acid can shift COM towards COD crystallization, which may confer a therapeutic effect as COD has lower affinity to epithelial cell membrane molecules and may thereby reduce intratubular retention. 11 , 45 , 46 …”

Section: Discussionmentioning

confidence: 99%

Application of metabolomics in urolithiasis: the discovery and usage of succinate

Zhang

Lei

Jiang

et al. 2023

Sig Transduct Target Ther

View full text Add to dashboard Cite

Urinary stone is conceptualized as a chronic metabolic disorder punctuated by symptomatic stone events. It has been shown that the occurrence of calcium oxalate monohydrate (COM) during stone formation is regulated by crystal growth modifiers. Although crystallization inhibitors have been recognized as a therapeutic modality for decades, limited progress has been made in the discovery of effective modifiers to intervene with stone disease. In this study, we have used metabolomics technologies, a powerful approach to identify biomarkers by screening the urine components of the dynamic progression in a bladder stone model. By in-depth mining and analysis of metabolomics data, we have screened five differential metabolites. Through density functional theory studies and bulk crystallization, we found that three of them (salicyluric, gentisic acid and succinate) could effectively inhibit nucleation in vitro. We thereby assessed the impact of the inhibitors with an EG-induced rat model for kidney stones. Notably, succinate, a key player in the tricarboxylic acid cycle, could decrease kidney calcium deposition and injury in the model. Transcriptomic analysis further showed that the protective effect of succinate was mainly through anti-inflammation, inhibition of cell adhesion and osteogenic differentiation. These findings indicated that succinate may provide a new therapeutic option for urinary stones.

show abstract

“…When the amount of oxidants exceeds that of antioxidants, oxidative stress occurs, causing an imbalance between oxidation and antioxidation. Then tissues are damaged and tissue structures are destroyed, resulting in inflammation [ 18 ]. Herein, compared with the normal group, the colon tissues and cells were arranged loosely and disorderly, the morphology of mucosal epithelium was changed, there was a small amount of inflammatory cell infiltration, the levels of IL-1β, TGF-β, TNF-α, D-lactate, and p-NF-κB p65 protein and the fecal water content significantly rose, and the levels of ZO-1 and occludin declined in model and PF groups.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats

et al. 2023

View full text Add to dashboard Cite

Background Irritable bowel syndrome (IBS) is a functional bowel disorder (FBD). Objectives To assess the therapeutic effects of paeoniflorin (PF) on IBS in rats. Method Sixty male Sprague–Dawley rats were randomly divided into normal, model, positive drug, low-dose PF, medium-dose PF and high-dose PF groups (n = 10). After gavage for 2 consecutive weeks, the effect of PF on abdominal pain symptoms was assessed based on the abdominal withdrawal reflex (AWR) score, fecal water content and pathological changes in colon tissues. D-lactate, interleukin-1β (IL-1β), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay, and phosphorylated nuclear factor kappa B (p-NF-κB) p65 was detected by Western blotting. The abundance and diversity changes of intestinal flora were explored using 16S ribosomal RNA sequencing. Result In PF groups, the mucosal morphology of colon tissues was intact, and the glands were arranged neatly and structured clearly, without obvious inflammatory cell infiltration. Compared with the model group, PF groups had significantly elevated pain threshold, and mRNA and protein levels of zonula occludens-1 (ZO-1) and occludin, decreased AWR score at 20 mmHg pressure, fecal water content, mRNA levels of IL-1β, TGF-β, and TNF-α, protein level of p-NF-κB p65 and level of serum D-lactate, and reduced levels of serum IL-1β, TGF-β, and TNF-α ( p < 0.05, p < 0.01). PF groups had higher abundance of Lactobacillus , Akkermansia , Alistipes , and Bacteroides , but lower abundance of Desulfovibrio , Parasutterella , and Enterococcus than those of the model group. Conclusions PF exerts therapeutic effects on IBS in rats probably by regulating the intestinal flora, and then up-regulating the expressions of ZO-1 and occludin in colon tissue while down-regulating the levels of IL-1β, TGF-β, TNF-α, D-lactate and p-NF-κB p65.

show abstract

Modulation of Rat Kidney Stone Crystallization and the Relative Oxidative Stress Pathway by Green Tea Polyphenol

Cited by 22 publications

References 26 publications

Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Bushen Huashi decoction against kidney stones

Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Bushen Huashi decoction against kidney stones

Application of metabolomics in urolithiasis: the discovery and usage of succinate

Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats

Contact Info

Product

Resources

About