Modulation of PPARα‐thermogenesis gut microbiota interactions in obese mice administrated with zingerone

Abstract

Background This study aimed to uncover the potential effects of zingerone (ZIN), one of the bioactive compounds in ginger, on the development of obesity as well as the mechanisms responsible for these effects in C57BL/6J mice fed with a high‐fat diet (HFD). Results Supplementation with 0.2% (wt/wt) zingerone for 16 weeks significantly reduced the final body weight, liver weight, and epididymal white adipose tissue (eWAT) weight without changing the food intake of the mice when compared with the HFD group. The hyperlipidemia of HFD‐fed mice was ameliorated after zingerone administration, including decreased plasma triacylglycerol (TG) and total cholesterol (TC) level. The lipid content in liver was lower and the adipocyte size in eWAT and inguinal white adipose tissue (iWAT) was smaller in HFD + ZIN‐fed mice compared with HFD group. Zingerone also binds with nuclear hormone receptor peroxisome proliferator‐activated receptor alpha (PPARα) with an optimal docking energy of −7.31 kJ/mol. Uncoupling protein 1 (UCP1), PPAR‐γ coactivator‐1α (PGC‐1α), and PR domain containing 16 (PRDM16), the downstream genes of PPAR which are related to thermogenic function of adipocytes, were significantly increased in both brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) after zingerone administration, in comparison with HFD fed mice. Zingerone intake also restructured the community composition of gut microbiota. The ratio of Firmicutes to Bacteroidetes was decreased, and the relative abundance of Akkermansia_mucinphila was increased. Conclusion Zingerone can attenuate obesity and related symptoms in HFD‐fed mice, probably through the modulation of PPARα‐thermogenesis‐gut microbiota interactions. © 2022 Society of Chemical Industry.

Effects of maternal advanced lipoxidation end products diet on the glycolipid metabolism and gut microbiota in offspring mice

“…From the relative abundance of gut microbial composition, it can be observed that maternal HALEs diet resulted in significant changes in the gut microbiota of offspring, characterized by an increased Firmicutes/Bacteroidetes ratio at the phylum level. Previous studies have shown that the relative proportion of Bacteroidetes was reduced in obese mice ( 38 ), and the changes in Firmicutes/Bacteroidetes ratios have been closely associated with metabolic disorders, such as obesity and diabetes ( 39 ). In this study, the maternal HALEs diet significantly increased the abundance of Dubosiella, and Dubosiella had significantly increased abundance in high fat diet feeding mice, suggesting its specific role in the obesity-related metabolic phenotype ( 40 ).…”