Modulation of Sex Expression in Four Forms of Watermelon by Gibberellin, Ethephone and Silver Nitrate

Zhang, Jie; Shi, Jianting; Ji, Gaojie; Zhang, Haiying; Gong, Guoyi; Guo, Sheng; Ren, Yi; Fan, Jianguang; Tian, Shouwei; Xu, Yong

doi:10.1016/j.hpj.2017.07.010

Cited by 33 publications

(26 citation statements)

References 25 publications

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“…However, in contrast to other cucurbit species, treatment of watermelon with ethylene-releasing agents results in the suppression of ovary development (Rudich and Zamski, 1985). Recent studies have demonstrated that ethylene inhibits the transition from male to female flowering and reduces the number of pistillate flowers per plant in watermelon (Manzano et al, 2014;Zhang et al, 2017). In this study, as shown by the DEGs depicted in Table S5, the ethylene biosynthesis gene ACS was upregulated in the gynoecious lines.…”

Section: Discussionmentioning

confidence: 52%

“…The watermelon explants were transformed according to our method (Tian et al, 2017;Zhang et al, 2017). Briefly, cotyledons without embryos were cut into pieces and used as explants.…”

Section: Construction and Transformation Of The Crispr/cas9 Vectormentioning

confidence: 99%

“…Meanwhile, treatments with ethylene inhibitors favour the development of male flowers (Byers et al, 1972;Den Nijs and Visser, 1980;Manzano et al, 2011). However, the effect of ethylene in watermelon is opposite to in the other cucurbit species: ethephon treatments have a masculinizing effect in watermelon (Manzano et al, 2014;Zhang et al, 2017). Recent studies have demonstrated that ethylene differentially modulates two sex-related processes: the earliness and number of female flowers per plant (sex expression) and the development of individual floral buds (sex determination) (Manzano et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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A unique chromosome translocation disrupting ClWIP1 leads to gynoecy in watermelon

Zhang

Guo

et al. 2019

The Plant Journal

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Summary To understand sex determination in watermelon (Citrullus lanatus), a spontaneous gynoecious watermelon mutant, XHBGM, was selected from the monoecious wild type XHB. Using map‐based cloning, resequencing and fluorescence in situ hybridization analysis, a unique chromosome translocation between chromosome 2 and chromosome 3 was found in XHBGM. Based on the breakpoint location in chromosome 2, a putative C2H2 zinc finger transcription factor gene, ClWIP1 (gene ID Cla008537), an orthologue of the melon gynoecy gene CmWIP1, was disrupted. Using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated system 9 to edit ClWIP1, we obtained gynoecious watermelon lines. Functional studies showed that ClWIP1 is expressed specifically in carpel primordia and is related to the abortion of carpel primordia in early floral development. To identify the cellular and metabolic processes associated with ClWIP1, we compared the shoot apex transcriptomes of two gynoecious mutants and their corresponding wild types. Transcriptome analysis showed that differentially expressed genes related to the ethylene and cytokinin pathways were upregulated in the gynoecious mutants. This study explores the molecular mechanism of sex determination in watermelon and provides a theoretical and technical basis for breeding elite gynoecious watermelon lines.

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Section: Discussionmentioning

confidence: 52%

“…The watermelon explants were transformed according to our method (Tian et al, 2017;Zhang et al, 2017). Briefly, cotyledons without embryos were cut into pieces and used as explants.…”

Section: Construction and Transformation Of The Crispr/cas9 Vectormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A unique chromosome translocation disrupting ClWIP1 leads to gynoecy in watermelon

Zhang

Guo

et al. 2019

The Plant Journal

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“…A research in watermelon, another member of Cucurbitaceae showed that external treatments with ethepon inhibits the transition from male to female flowering and reduces the number of pistillate flowers per plant, which runs contrary to findings in other Cucurbit species [15]. Other research showed that the ethepon treatments reduced the percentage of female flowers and delayed the occurrence of the first female flower [13]. The two researches showed different responses in watermelon flowering mechanism affected by ethepon treatment compared to other member Cucurbit species.…”

Section: Resultsmentioning

confidence: 89%

“…This gene is involved in the formation of female or hermaphrodite flower by stimulating the pistil formation. A research in watermelon also showed that the the transcription level of ACS homologous is recognized as andromonoecious gene in plant growth regulator and silver nitrate treatments [13].…”

Section: Resultsmentioning

confidence: 98%

The effect of ethepon treatment on the formation of flower in melon (Cucumis melo L.)

Daryono

Prasetya

Sumarlina

et al. 2018

Digital Press Life Sciences

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Melon is a potential horticultural crop which the production is increased significantly each year in Indonesia. However, a melon plant that has been widely developed in Indonesia faced a problem due to the high tropical temperature that affected the fall of flower causing failure in fruit production. In addition, the variation of sex expression in melon flower is important for genetic analysis and breeding programs. Ethepon is one of plant chemical growth regulator that is known to change the sex expression of the plant by increasing the number of female flower in monoecious plant especially Cucurbitaceae. This research aimed to analyze the effect of ethepon treatment on the sex determination of melon flower. This study was conducted by 3 stages treatment of ethepon during March -July 2014, namely when 2, 5, and 7 weeks after planting. Split-plot design has been used with the main plots were arranged in a completely randomized design by cultivating different melon cultivars i.e: Melodi Gama 1, Melodi Gama 3, Bartek, and PI 371795. The data were analyzed by F-test and Duncan Multiple Range Test (DMRT) with significance level at 5 % using software SAS 9.3. The result showed that ethepon treatment affected the formation of melon flower by increasing the number of female or hermaphrodite flower and decreasing the number of male flowers, especially in the concentration of 75 ppm and 100 ppm but with different responses by different cultivar.

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Role of Ethylene in Flower and Fruit Development

Martínez¹,

García²,

Jamilena³

2022

Ethylene in Plant Biology

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Modulation of Sex Expression in Four Forms of Watermelon by Gibberellin, Ethephone and Silver Nitrate

Cited by 33 publications

References 25 publications

A unique chromosome translocation disrupting ClWIP1 leads to gynoecy in watermelon

A unique chromosome translocation disrupting ClWIP1 leads to gynoecy in watermelon

The effect of ethepon treatment on the formation of flower in melon (Cucumis melo L.)

Role of Ethylene in Flower and Fruit Development

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