Modulation of Stem Cell Progeny by Probiotics during Regeneration of Gastric Mucosal Erosions

Abstract

Patients with gastric mucosal erosions are predisposed to chronic gastritis, ulcer or even cancer. The repair of mucosal erosions involves several events including proliferation of gastric epithelial stem cells. The aim of this study was to investigate the effects of the probiotic mixture of De Simone Formulation on gastric epithelial stem cell lineages in mouse models of gastric mucosal erosions. Gastric erosions were induced by a single oral gavage of 80% ethanol containing 15 mg/mL acetylsalicylic acid (5 mL/kg) following a daily dose of probiotic mixture (5 mg/day/mouse) for 10 days. In another protocol, erosions were induced by a daily gavage of acetylsalicylic acid (400 mg/kg/day/mouse) for 5 days before or after daily administration of probiotic mixture for 5 days. Control mice received water gavage for 10 days. All mice were injected with bromodeoxyuridine two hours before sacrifice to label S-phase cells. The stomachs of all mice were processed for histological examination, lectin binding, and immunohistochemical analysis. The results reveal that mice that received probiotics before or after the induction of erosion showed a decrease in erosion index with an increase in gastric epithelial stem/progenitor cell proliferation and enhanced production of mucus, trefoil factors, and ghrelin by mucous and enteroendocrine cell lineages. These mice also showed restoration of the amount of H+,K+-ATPase and pepsinogen involved in the production of the harsh acidic environment by parietal and chief cell lineages. In conclusion, this study demonstrates the beneficial effects of probiotics against gastric mucosal erosion and highlights the involvement and modulation of proliferative stem cells and their multiple glandular epithelial cell lineages.

Synbiotic Combination between Lactobacillus paracasei VL8 and Mannan-Oligosaccharide Repairs the Intestinal Barrier in the Dextran Sulfate Sodium-Induced Colitis Model by Regulating the Intestinal Stem Cell Niche

“…18 Athiyyah's study showed that L. plantarum IS-10506 activates ISCs for inflammation mitigation, 19 while combined probiotic therapy has been effective in treating stomach erosions by modulating stem and secretory cell lineages. 20 Furthermore, dietary raffinose supports Lactobacillus reuteri in the gut microbiota, promoting ISC maintenance in stress conditions through a metabolic loop involving fructose and glycolysis. 21 The novel approach of plasmid-mediated IL-22 delivery in second-generation probiotics has shown promise in preserving intestinal crypt stem cells and enhancing structural integrity.…”

Xxvii International Symposium on Morphological Sciences “Cell, Tissue, Organs – Experience, Innovation and Progress”

“…A large number of speakers (12) and listeners (40) were united in the topic: «Clinical anatomy; morphological aspects of the somatic pathology; cell biology and biochemistry in the modern clinical research» (section 2).…”