Modulation of superoxide dismutase (SOD) isozymes by organ development and high long-term salinity in the halophyte Cakile maritima

Houmani, Hayet; Rodríguez-Ruiz, Marta; Palma, José M.; Abdelly, Chédly; Corpas, Francisco J.

doi:10.1007/s00709-015-0850-1

Cited by 64 publications

(38 citation statements)

References 69 publications

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“…The number of SOD unigenes are different in plants reported. For example, there are 7 SODS in Arabidopsis [33], 10 SODs in Cakile maritima [34], and 4 SODs in pepper [35]. A total of 29 and 18 SOD genes have been identified in Brassica juncea and Brassica rapa, respectively [36].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage

Pang

Liu

et al. 2020

Antioxidants

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It has been revealed by us that superoxide scavenging is a new activity of trypsin. In this study, the synergistic mechanisms of trypsin and superoxide dismutases (SODs) were evaluated in Hylocereus undatus (pitaya). Trypsin significantly improved the storage quality of H. undatus, including weight loss impediment and decrease of cellular injury. The regulatory mechanisms of 16 SOD genes by trypsin were revealed using transcriptomic analysis on H. undatus. Results revealed that important physiological metabolisms, such as antioxidant activities or metal ion transport were induced, and defense responses were inhibited by trypsin. Furthermore, the results of protein-protein interaction (PPI) networks showed that besides the entire ROS network, the tiny SODs sub-network was also a scale-free network. Cu/Zn SODs acted as the hub that SODs synergized with trypsin during the storage of H. undatus.Trypsin is a widely used protease. It has been confirmed that trypsin significantly impacts on the free radical scavenging activities of flavonoids [8]. Our previous results also showed that trypsin scavenges superoxide anions (O 2 • − ) and protects cells [9].In the area of phytopathology, transcriptomic analysis has been successful used on lots of plants [10][11][12][13]. However, few of transcriptomic works have been focused on the preservation of fruits. The mechanisms of betalain biosynthesis have been investigated in H. undatus [2], and the concision analysis of antioxidant system induced by trypsin has also been investigated in our previous paper [14]. Considering the superoxide scavenging activity of trypsin, the function and synergistic mechanisms of trypsin with SODs impact on the quality of H. undatus during storage is an interesting work. The mechanisms of postharvest quality of fruits still need more works.Protein-protein interaction (PPI) networks analyses help us to figure out the regulatory mechanisms of proteins [15]. Plugins of Cytoscape, including NetworkAnalyzer, Molecular Complex Detection (MCODE), and cytoHubba, could score and rank the nodes or obtain clusters in the PPI network [16].In the current work, the regulatory mechanisms of trypsin on the H. undatus by cooperating with SODs were investigated. The changes in expression of SODs of H. undatus peels were analyzed. Analyses of gene ontology (GO) and KEGG enrichment of ROS or SOD related genes were performed, and the PPI network of ROS related genes and sub-network of SODs and their first neighbors were constructed. The hub genes of SODs regulated by trypsin during storage were further screened by cytoHubba and MCODE of cytoscape.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage

Pang

Liu

et al. 2020

Antioxidants

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“…Most plant tissues contain at least one Fe-SOD, although in this work, we were not able to detect any Fe-SOD activity in pomegranate. So, in order to confirm the absence of this isozyme, western blotting assays using an antibody against Fe-SOD from pepper (Capsicum annuum) fruits [54], (also characterized in other plant species like Olea europaea [53] and Cakile maritima [55]), were carried out. Interestingly, one cross-reacting band of about 23 kDa was detected in all seeds and juices from F-GR and E-A samples.…”

Section: Resultsmentioning

confidence: 99%

“…Both pomegranate populations share the same isozyme profile but the activity of some of them was much higher in E-A seeds than in F-GR. In previous works, it was found that SOD activity was used as index of the cultivar and ripening stage, and it was described that this enzyme also participated in the response against diverse unfavorable conditions [14][15][16][17][18][19]55]. Recently, by iTRAQ analysis, the presence of a SOD from the Fe,Mn family has been reported [32], but no CuZn-SODs have been described so far.…”

Section: Discussionmentioning

confidence: 99%

“…For immunoblots, proteins were separated by SDS-PAGE as above and then transferred onto PVDF membranes using a Trans-Blot SD (Bio-Rad) equipment and 10 mM N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), pH 11.0, as transfer buffer [52]. An antibody against pepper Fe-SOD (dilution 1:1000), obtained and characterized in our laboratory [53][54][55], was used and the detection of the antibody-recognizing proteins was achieved using the Clarity TM Western ECL Substrate (Bio-Rad) kit following the manufacturer's instructions.…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

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Pomegranate (Punica granatum L.) Fruits: Characterization of the Main Enzymatic Antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System

et al. 2019

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Pomegranate (Punica granatum L.) is a common edible fruit. Its juice can be used as a source of antioxidative compounds, primarily polyphenols and vitamin C, in addition to other vitamins and minerals. Nevertheless, little is still known about how the enzymatic machinery, mainly that related to oxidative metabolism, is influenced by the genotype and the environmental and climate conditions where pomegranate plants grow. In this work, seeds and juices from two pomegranate varieties (Valenciana and Mollar) grown in two different Spanish locations were assayed. Both varieties showed clear differences in their respective polypeptide profiles. The analysis of the isoenzymatic superoxide dismutase (SOD) activity pattern displayed one Mn-SOD and five CuZn-SODs (I–V) whose abundances depended on the variety. Furthermore, by immunoblot assays, at least one additional Fe-SOD with a subunit size of about 23 kDa was also detected in both varieties. Besides this, the presence of the H2O2-scavenging peroxisomal catalase in seeds and juice indicates that an active metabolism of reactive oxygen species (ROS) takes place in this fruit, but the two pomegranate varieties showed opposite activity profiles. The activities of the main NADPH-regenerating enzymes, including glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphlogluconate dehydrogenase (6PGDH), NADP-dependent isocitrate dehydrogenase (NADP-ICDH), and NADP-dependent malic enzyme (NADP-ME), were studied in the same plant materials, and they behaved differently depending on the genotype. Finally, our data demonstrate the presence of two specific enzymes of the peroxisomal glyoxylate cycle, malate synthase (MS) and isocitrate lyase (ICL). These enzymes participate in oilseeds by channeling the lipid catabolism to the carbohydrate synthesis for further use in seed germination and early seedling growth. The results obtained in this work indicate that a similar mechanism to that reported in oilseeds may also operate in pomegranate.

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“…In recent years, the characterization of plants has been conducted not only based on morphological characters but also the isozyme banding pattern as reported by Premoli (2003) and Zolfghari et al (2010). The use of plant isozyme in the characterization of plants have been conducted widely (Rejon et al 2012;Houmani et al 2016;Hartanti et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018

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Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

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Modulation of superoxide dismutase (SOD) isozymes by organ development and high long-term salinity in the halophyte Cakile maritima

Cited by 64 publications

References 69 publications

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage

Pomegranate (Punica granatum L.) Fruits: Characterization of the Main Enzymatic Antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System

Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

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