Modulation of the biological effects of glucosinolates by inulin and oat fibre in gnotobiotic rats inoculated with a human whole faecal flora

Roland, Nathalie; Rabot, Sylvie; Nugon-Baudon, Lionelle

doi:10.1016/0278-6915(96)00038-5

Cited by 35 publications

(28 citation statements)

References 24 publications

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“…This protocol indicated a bioantimutagenic mode of action, which reinforced the data from the pre-treatment protocol and the remarks made by other authors in other biological assays (Roland et al, 1993(Roland et al, , 1994(Roland et al, , 1995(Roland et al, , 1996Mauro et al, 2013). This protocol suggests and confirms the action of inulin modulating the repair system.…”

Section: Discussionsupporting

confidence: 86%

“…Further studies are needed so that the interaction between inulin and DNA can be better described and so the mechanism of action can be confirmed in test systems for mammals, especially in vitro. Therefore, taking into account data from Roland et al (1993Roland et al ( , 1994Roland et al ( , 1995Roland et al ( , 1996, Roberfroid (2005), , and the results presented in this study, we suggest that inulin, when consumed in the recommended concentrations, can be used as an adjuvant in chemotherapies, acting as an antimutagenic agent or even as a functional food that is capable of preventing the occurrence of mutations related to the origin of neoplasm.…”

Section: Discussionmentioning

confidence: 74%

“…This suggests that inulin can modulate repair enzymes of the cellular DNA as suggested by Roland et al (1993Roland et al ( , 1994Roland et al ( , 1995Roland et al ( , 1996 and Mauro et al (2013). Therefore, we supposed that the inulin entered the meristematic cells of A. cepa and modulated the repair system, making it more efficient when preventing or repairing the damage caused by the alkylating action of MMS.…”

Section: Discussionmentioning

confidence: 91%

“…However, the reductions in frequencies of mutations studied reflect the possible mechanisms of this fiber in the activation of antioxidant and detoxifying enzymes, such as glutathione S-transferase and glucuronosyltransferase (Roland et al, 1993(Roland et al, , 1994(Roland et al, , 1995(Roland et al, , 1996. Fiskesjö (1993a) affirmed that the MI of apical meristems of A. cepa facing the chemical substances is considered normal when there is inhibition of cellular division up to 36%.…”

Section: Discussionmentioning

confidence: 92%

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Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Mauro¹,

Pesarini²,

Marin-Morales³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control -seed germination in distilled water; positive control -aqueous solution of methyl methanesulfonate (10 μg/mL MMS); mutagenicity -aqueous solutions of inulin (0.015, 0.15, and 1.50 μg/mL); and antimutagenicity -associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pretreatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pretreatment, suggesting activity by bioantimutagenesis and desmutagenesis.The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 92%

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Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Mauro¹,

Pesarini²,

Marin-Morales³

et al. 2014

Genet. Mol. Res.

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“…Transplantation of human gut microbiota into surrogate hosts, such as mice or rats, permits the generation of a human flora-associated (HFA) animal model with an established human-derived gut microbiota in place of its indigenous microbial ecosystem. Such HFA animal model has significantly facilitated progress in researches of human gut ecology, metabolism and immunity (Hazenberg et al, 1981;Roland et al, 1996;Oozeer et al, 2002;Gerard et al, 2004;Wilcks et al, 2004). However, owing to the significant differences in basic anatomy and physiology of rodents to human, key members of human gut microbiota such as bifidobacteria do not colonize the rodent gut; and rodents do not develop a full repertoire of immune responses unless some rodent-specific gut bacteria are reintroduced back into the gut (Raibaud et al, 1980;Imaoka et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Inter-species transplantation of gut microbiota from human to pigs

et al. 2007

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Direct research on gut microbiota for understanding its role as 'an important organ' in human individuals is difficult owing to its vast diversity and host specificity as well as ethical concerns. Transplantation of human gut microbiota into surrogate hosts can significantly facilitate the research of human gut ecology, metabolism and immunity but rodents-based model provides results with low relevance to humans. A new human flora-associated (HFA) piglet model was hereby established taking advantage of the high similarity between pigs and humans with respect to the anatomy, physiology and metabolism of the digestive system. Piglets were delivered via cesarean section into a SPF-level barrier system and were inoculated orally with a whole fecal suspension from one healthy 10-year-old boy. The establishment and composition of the intestinal microbiota of the HFA piglets were analyzed and compared with that of the human donor using enterobacterial repetitive intergenic consensus sequence-PCR fingerprinting-based community DNA hybridization, group-specific PCR-temperature gradient gel electrophoresis and real-time PCR. Molecular profiling demonstrated that transplantation of gut microbiota from a human to germfree piglets produced a donor-like microbial community with minimal individual variation. And the microbial succession with aging of those ex-germfree piglets was also similar to that observed in humans. This HFA model provides a significantly improved system for research on gut ecology in human metabolism, nutrition and drug discovery.

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The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods

Mithen

Dekker

Verkerk

et al. 2000

J. Sci. Food Agric.

430

300

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The glucosinolates are a large group of sulphur‐containing compounds which occur in all the economically important varieties of Brassica vegetable. Their common structure comprises a β‐D‐thioglucose group, a sulphonated oxime moiety and a variable side‐chain derived from methionine, tryptophan or phenylalanine. When the plant tissue is damaged the glucosinolates are hydrolysed by the endogenous enzyme ‘myrosinase’ (thioglucoside glycohydrolase EC 3:2:3:1), to release a range of breakdown products including the bitter, biologically active isothiocyanates. Although these compounds exert antinutritional effects in animals there is also substantial evidence that they are the principal source of anticarcinogenic activity in Brassica vegetables, and this provides a strong motive for the manipulation of glucosinolate levels in vegetables for human consumption. This review provides an overview of the evidence for a beneficial role for glucosinolates in human health, and describes the current state of knowledge regarding the genetics and biosynthesis of glucosinolates, their chemical analysis, their behaviour during cooking and processing, and their bioavailability to humans. As the genetic basis of glucosinolate biosynthesis becomes more apparent, and tools for marker‐assisted plant breeding become more available, the selective breeding of horticultural brassicas with different levels and types of glucosinolates, whether by conventional means or genetic manipulation, is becoming a practical possibility. However before this strategy becomes commercially viable, the health benefits of glucosinolates for human beings must be unequivocally established. © 2000 Society of Chemical Industry

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Modulation of the biological effects of glucosinolates by inulin and oat fibre in gnotobiotic rats inoculated with a human whole faecal flora

Cited by 35 publications

References 24 publications

Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture

Inter-species transplantation of gut microbiota from human to pigs

The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods

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