Modulation of the number of membrane-bound auxin-binding sites during the growth of batch-cultured tobacco cells

Abstract

We studied the modulation of the number of membrane-bound naphthaleneacetic acid (NAA)-binding sites during the growth cycle of tobacco cells in batch culture. Both cell number and specific NAA-binding increased exponentially, but at different rates and for different periods. This caused a characteristic modulation of the number of binding sites per cell during the growth cycle: During the first day of the lag phase this number decreased; in the exponential phase it rose markedly, and in the stationary phase it was constant.

Molecular cloning and structural analysis of a gene from Zea mays (L) coding for a putative receptor for the plant hormone auxin

“…Most importantly, disappearance of these binding sites appears to correlate with an inhibition of elongation of mesocotyl segments (Walton and Ray, 1981). Furthermore, in N. tabacum, the appearance of a similar binding site seems to correlate with root regeneration (Vreugdenhill et al, 1981;Maan et al, 1985).…”

An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum)

“…Hormone receptors are thought to be an important determinant of tissue sensitivity. The levels of putative hormone receptors can be modulated by external factors (Walton and Ray 1981) and can vary during cell development (Vreugdenhil et al 1981). If the level of receptors for one or more of the hormones involved in the tuber initiation also changes during stolon development, this might be another source of variation among stolons.…”

In vitro binding of auxin to particulate fractions from the shoots of dark-grown wheat seedlings

“…It is assumed that these binding sites play a key role in the recognition of the informational signal carried by auxins and in its transduction to the effective structures in the cell. 25 ]. The idea that these binding sites have a receptor function is supported by their low capacity, high affinity and specificity for auxin-active substances, and also by the dependence of their capacity on the actual physiological state of a given plant material [e.g.…”