Modulation of the transcriptional rate of Fcγ receptor mRNA in human mononuclear phagocytes

Comber, Paul G.; Lentz, Vicky M.; Schreiber, Alan D.

doi:10.1016/0008-8749(92)90335-m

Cited by 18 publications

(10 citation statements)

References 54 publications

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“…Such divergent observations may well reflect differences in culture media and conditions. No other reports of the effects of glucocorticoid upon FcRn expression have been made, although glucocorticoids have been shown to lead to a loss of Fcg receptor expression in intestinal enterocytes of newborn suckling rats (35) or reductions in vitro of both basal and IFNg-induced expressions of FcgRI in leucocyte cell lines, although such effects seem to be cell specific (36) and indeed Fc receptor subtype specific (37).…”

Section: Discussionmentioning

confidence: 97%

Expression and Transport Functionality of FcRn within Rat Alveolar Epithelium: A Study in Primary Cell Culture and in the Isolated Perfused Lung

et al. 2006

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Section: Discussionmentioning

confidence: 97%

Expression and Transport Functionality of FcRn within Rat Alveolar Epithelium: A Study in Primary Cell Culture and in the Isolated Perfused Lung

et al. 2006

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“…In addition, Interleukin 4 has been shown to reduce the expression of all three FcγR (20). Modulation of FcγRI in particular has been shown to be susceptible to treatment with glucocorticoids, which oppose the effect of IFN-γ (24). Furthermore, neither IFN-γ nor dexamethasone affects mRNA stability of FcγR (24, 25).…”

Section: Discussionmentioning

confidence: 99%

“…Modulation of FcγRI in particular has been shown to be susceptible to treatment with glucocorticoids, which oppose the effect of IFN-γ (24). Furthermore, neither IFN-γ nor dexamethasone affects mRNA stability of FcγR (24, 25). At the molecular level, transcriptional regulation of FcγRI has been shown to be dependent upon two main factors: PU.1 and Stat1.…”

Section: Discussionmentioning

confidence: 99%

Itraconazole, a Commonly Used Antifungal, Inhibits Fcγ Receptor–Mediated Phagocytosis

Niño

Cauvi

Maio

2014

Shock

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Itraconazole (ICZ)‡ is commonly used for the treatment of fungal infections, particularly in immunocompromised patients. In addition, ICZ has been recently found to have anti-angiogenic effects and is currently being tested as a new chemotherapeutic agent in several cancer clinical trials. We have previously shown that ICZ impaired complex N-linked glycosylation processing, leading to the accumulation of high-mannose glycoproteins on the surface of macrophages. This investigation was directed at determining the effects of ICZ on phagocytosis as a major function of macrophages. We found a significant decrease in the phagocytosis of opsonized bacterial particles in ICZ-treated murine macrophages in comparison with non-treated macrophages. Furthermore, the impairment of phagocytosis was associated with a decrease in cell surface expression of Fcγ receptors (FcγR) as well as alteration of their glycosylation pattern. Concomitantly, a reduction in all three isoforms of the FcγR family (i.e., Fcgr1, Fcgr2 and Fcgr3) mRNA levels was observed after incubation with ICZ. The effect of ICZ on phagocytosis and FcγR expression was reversed by addition of LDL. These studies indicate that ICZ treatment certainly has a dramatic effect on macrophage function, which could result in a potential impairment of the immune system's ability to respond to pathogens and may lead to elevated incidence of infections.

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“…An hCG concentration of 1.0 ng/ml was used so that we could maintain adequate levels of receptor transcripts, especially in the control and in the initial treatment samples. After culturing with or without hCG, 5 pg/ml actinomycin D was added to arrest further transcription [10,20]. The 0-time samples were separated, and the remaining samples were incubated for 3-24 h. At the end of incubation, total cellular RNA was isolated for Northern blotting for LH/hCG receptors and GAPDH mRNAs.…”

Section: Measurement Of the Stability Of Transcriptsmentioning

confidence: 99%

Homologous Down-Regulation of Luteinizing Hormone/Chorionic Gonadotropin Receptors by Increasing the Degradation of Receptor Transcripts in Human Uterine Endometrial Stromal Cells

Han¹,

Lei²,

Rao³

1997

Biology of Reproduction

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We investigated the possible homologous down-regulation of LH/hCG receptors in human uterine endometrial stromal cells. The cells contained a major 4.3-kilobase (kb) and minor 3.6-kb, 2.4-kb, 1.8-kb, and 1.0-kb transcripts of receptors and an 80-kDa receptor protein that can bind [125I]hCG. Culturing these cells with increasing concentrations of highly purified hCG resulted in a dose-dependent significant decrease in steady-state levels of all the receptor transcripts, the 80-kDa receptor protein, and [125I]hCG binding as compared to the control values. The hCG effect was hormone specific and required the conformation of native hormone. The decrease in steady-state receptor transcript levels by hCG was not due to a decrease in the transcription rate of the LH/hCG receptor gene. It was rather due to a significant decrease in the half-life of receptor transcripts from 40.1 +/- 12.4 h in the control to 13.3 +/- 3.6 h after treatment. The homologous down-regulation observed in the present study may potentially explain low endometrial receptor levels in the postmenopausal human endometrium.

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Modulation of the transcriptional rate of Fcγ receptor mRNA in human mononuclear phagocytes

Cited by 18 publications

References 54 publications

Expression and Transport Functionality of FcRn within Rat Alveolar Epithelium: A Study in Primary Cell Culture and in the Isolated Perfused Lung

Expression and Transport Functionality of FcRn within Rat Alveolar Epithelium: A Study in Primary Cell Culture and in the Isolated Perfused Lung

Itraconazole, a Commonly Used Antifungal, Inhibits Fcγ Receptor–Mediated Phagocytosis

Homologous Down-Regulation of Luteinizing Hormone/Chorionic Gonadotropin Receptors by Increasing the Degradation of Receptor Transcripts in Human Uterine Endometrial Stromal Cells

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