Modulation of tumor necrosis factor (TNF) receptor expression during monocytic differentiation by glucocorticoids

Abstract

These results indicate differential regulation of the two types of TNF receptors at the mRNA and protein level during monocytic differentiation. Glucocorticoids interfered with mRNA expression of TNF-R80 and protein expression of TNF-R60, but the rather limited effect leaves the question of its functional relevance open. In contrast to other cytokine systems, TNF receptors do not appear to be major targets of glucocorticoid action.

The glucocorticoid inhibition of LFA-1 and CD2 expression by human mononuclear cells is reversed by IL-2, IL-7 and IL-15

“…A further key issue, which has already been addressed in the literature, regards the lack of effect of GC on the receptors for the other cytokines tested. It has emerged that the expression of some relevant cytokine receptors is either not inhibited (TNF‐αR, IL‐1R) 21, 22 or enhanced (IL‐4R) 23 by GC, with no data on the IL‐10R. Therefore, it is highly unlikely thatthe lack of effect of TNF‐α, IL‐1β, and IL‐4 in our system is due to a GC‐induced down‐regulation of their respective receptors.…”

The characterization and role of leukemia cell-derived dendritic cells in immunotherapy for leukemic diseases

“…There are contradictory findings regarding the DC differentiation capacity of THP-1 cells. Although THP-1 cells have been noted to acquire DC-like properties upon stimulation with cytokines, THP-1 cells have a relatively low DC differentiation capacity since less than 5% of THP-1 cells express the classic myeloid DC marker CD1a (46,47). Moreover, THP-1 DCs fail to function like DCs, as indicated by the absence of allogeneic T-cell stimulatory capacity.…”