Experiments on particle-free and particle-laden flows with the same incoming velocity were conducted in a horizontal wind tunnel. Three cases of particle-laden flows with different degrees of particle near-wall motions and similar particle volume fractions, including top-release particles, local-laying sand beds and global-laying sand beds, were designed to investigate the effects of collision bounces and impact splashing on turbulence statistics. The top-released particles accelerate the fluid during gravitational settling, but weaken the intensity and reduce the probability of ‘ejection’ and ‘sweep’ events. This leads to a weakened Reynolds stress and a decreased scale of the outer spectral peak at the centre of the logarithmic region, indicating a concentration of energy at small scales. In contrast, the collision bounce and impact splashing slow the fluid but promote the ‘ejection and sweep’ cycle with larger intensity, and thus enhance the Reynolds stress. Meanwhile, the bouncing and splashing generate ascending particles that transport the kinetic energy upwards, resulting in more energetic very-large-scale motions further from the wall. This study reveals the importance of particle motions to turbulence, and contributes to a further insight into the interactions between particles and turbulence in two-phase flows with erodible surfaces.