Modulation of vascular function and anti-aggregation effect induced by (1 → 3) (1 → 6)-β-d-glucan of Saccharomyces cerevisiae and its carboxymethylated derivative in rats

Bezerra, Lorena Soares; Magnani, Marciane; Castro-Gómez, Raúl Jorge Hernán; Cavalcante, Hassler Clementino; Silva, Tays Amanda Felisberto da; Vieira, Renata Layne Paixão; Medeiros, Isac A.; Veras, Robson Cavalcante

doi:10.1016/j.pharep.2017.01.002

Cited by 20 publications

(19 citation statements)

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“…Regarding glucan NPs, to the best of authors’ knowledge, no studies of platelet aggregation have been reported. Nonetheless, Bezerra and colleagues reported a study where different concentrations of β- d -glucans led to the dose-dependent inhibition of collagen-induced platelet aggregation, making them therefore a possible drug to treat cardiovascular diseases in the future …”

Section: Resultsmentioning

confidence: 99%

“…Nonetheless, Bezerra and colleagues reported a study where different concentrations of β-D-glucans led to the dose-dependent inhibition of collagen-induced platelet aggregation, making them therefore a possible drug to treat cardiovascular diseases in the future. 53 For the following studies, a mouse macrophage cell line (RAW 264.7) was used since it is an easier model to study the toxicity of nanomaterials, in this case, to corroborate the cytotoxicity previously determined in human PBMCs and also to investigate the behavior of cells after glucan NPs uptake in terms of inflammatory markers such as nitric oxide (NO) and reactive oxygen species (ROS).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Safe-by-Design of Glucan Nanoparticles: Size Matters When Assessing the Immunotoxicity

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Marques

Jesus

et al. 2020

Chem. Res. Toxicol.

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Glucan (from Alcaligenes faecalis) is a polymer composed of β-1,3-linked glucose residues, and it has been addressed in different medical fields, namely in nanotechnology, as a vaccine or a drug delivery system. However, due to their small size, nanomaterials may present new risks and uncertainties. Thus, this work aims to describe the production of glucan nanoparticles (NPs) with two different sizes, and to evaluate the influence of the NPs size on immunotoxicity. Results showed that, immediately after production, glucan NPs presented average sizes of 129.7 ± 2.5 and 355.4 ± 41.0 nm. Glucan NPs of 130 nm presented greater ability to decrease human peripheral blood mononuclear cells and macrophage viability and to induce reactive oxygen species production than glucan NPs of 355 nm. Both NP sizes caused hemolysis and induced a higher metabolic activity in lymphocytes, although the concentration required to observe such effect was lower for the 130 nm glucan NPs. Regarding pro-inflammatory cytokines, only the larger glucan NPs (355 nm) were able to induce the secretion of IL-6 and TNF-α, probably due to their recognition by dectin-1. This higher immunomodulatory effect of the larger NPs was also observed in its ability to stimulate the production of nitric oxide (NO) and IL-1β. On the contrary, a small amount of Glu 130 NPs inhibited NO production. In conclusion, on the safe-by-design of glucan NPs, the size of the particles should be an important critical quality attribute to guarantee the safety and effectiveness of the nanomedicine.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Safe-by-Design of Glucan Nanoparticles: Size Matters When Assessing the Immunotoxicity

Colaço

Marques

Jesus

et al. 2020

Chem. Res. Toxicol.

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“…In addition, it has been reported that the daily oral treatment with CM-G (20 mg/kg) for eight days reduced interleukin 8 (IL-8), improved vascular response to nitric oxide and exhibited anti-aggregation activity. This suggests that CM-G could have a beneficial effect on the vascular system ( Bezerra et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

A Newly Isolated Carboxymethyl-Glucan (CM-G) Restores Depressed Baroreflex Sensitivity in Renovascular Hypertensive Rats

et al. 2018

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This study was designed to investigate the effects of a newly synthesized carboxymethyl-glucan (CM-G) on blood pressure (BP), baroreflex sensitivity (BRS) and sympathetic vascular modulation in renovascular hypertensive rats. Male Wistar rats were divided into four groups: Sham (n = 10); 2K1C (subjected to renal artery clipping to induce renovascular hypertension, n = 10); Sham + CM-G (treated with CM-G, n = 7) and 2K1C + CM-G (treated with CM-G, n = 7). The daily treatment with CM-G (40 mg/kg) was performed for 2 weeks. Blood pressure, heart rate (HR), systolic BP variability, baroreflex sensitivity (BRS) and sympathetic vascular tone were evaluated. After six weeks of renal artery clipping, 2K1C rats exhibited arterial hypertension (171 ± 11 vs. 118 ± 4 mmHg, p < 0.05), impaired BRS (-1.30 ± 0.10 vs. -2.59 ± 0.17 bpm.mmHg-1, p < 0.05) and enhanced sympathetic activity as shown by the hexamethonium test (-60 ± 5 vs. -33 ± 2 ΔmmHg, p < 0.05) when compared to sham rats. Oral administration of CM-G in renovascular hypertensive rats reduced hypertension (126 ± 4 vs. 171 ± 11 mmHg, p < 0.05) and improved the BRS (-2.03 ± 0.16 vs. -1.30 ± 0.10 bpm.mmHg-1, p < 0.05) in 2K1C rats when compared to placebo. Those effects seem to be caused by a reduction in sympathetic activity. The present study revealed for the first time that CM-G treatment reduces arterial hypertension and restores arterial baroreflex sensitivity via a reduction in the sympathetic tone in conscious renovascular hypertensive rats.

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“…The tissues were maintained in Krebs solution at 37 °C and aerated with 95% O 2 and 5% CO 2 (Carbogen, White Martins, Brazil). All the rings were subjected to a basal tension of approximately 1.0 g during a stabilization period of 60 min, exchanging the nutrient solution every 15 min [ 24 ]. After stabilization and viability verification, vascular endothelium was considered intact when the aortic rings showed ACh-induced relaxation greater than 70% after Phe (1 µM, Sigma Aldrich PHR1017, Burlington, MA, USA) contraction.…”

Section: Methodsmentioning

confidence: 99%

“…After stabilization and viability verification, vascular endothelium was considered intact when the aortic rings showed ACh-induced relaxation greater than 70% after Phe (1 µM, Sigma Aldrich PHR1017, Burlington, MA, USA) contraction. Endothelium removal was confirmed by a relaxation of less than 10% [ 24 ]. Next, isolated concentration–response curves for Phe (10 −9 − 10 −5 M), ACh (10 −9 − 10 −5 M, TCI America A008425G, Portland, OR, USA) and SNP (10 −12 − 10 −5 M, Sigma Aldrich 71778, Burlington, MA, USA) were obtained.…”

Section: Methodsmentioning

confidence: 99%

Effects of Baru Almond Oil (Dipteryx alata Vog.) Treatment on Thrombotic Processes, Platelet Aggregation, and Vascular Function in Aorta Arteries

et al. 2022

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Background: This study assessed the effects of Baru (Dipteryx alata Vog.) almond oil supplementation on vascular function, platelet aggregation, and thrombus formation in aorta arteries of Wistar rats. Methods: Male Wistar rats were allocated into three groups. The control group (n = 6), a Baru group receiving Baru almond oil at 7.2 mL/kg/day (BG 7.2 mL/kg, n = 6), and (iii) a Baru group receiving Baru almond oil at 14.4 mL/kg/day (BG 14.4 mL/kg, n = 6). Baru oil was administered for ten days. Platelet aggregation, thrombus formation, vascular function, and reactive oxygen species production were evaluated at the end of treatment. Results: Baru oil supplementation reduced platelet aggregation (p < 0.05) and the production of the superoxide anion radical in platelets (p < 0.05). Additionally, Baru oil supplementation exerted an antithrombotic effect (p < 0.05) and improved the vascular function of aorta arteries (p < 0.05). Conclusion: The findings showed that Baru oil reduced platelet aggregation, reactive oxygen species production, and improved vascular function, suggesting it to be a functional oil with great potential to act as a novel product for preventing and treating cardiovascular disease.

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Modulation of vascular function and anti-aggregation effect induced by (1 → 3) (1 → 6)-β-d-glucan of Saccharomyces cerevisiae and its carboxymethylated derivative in rats

Abstract: These findings suggest that CM-G modulate positively the vascular function, mainly in responses NO-dependent. CM-G and βG-Sc have an anti-aggregation effect, being CM-G more selective to ADP-induced platelet aggregation.

Cited by 20 publications

References 44 publications

Safe-by-Design of Glucan Nanoparticles: Size Matters When Assessing the Immunotoxicity

Safe-by-Design of Glucan Nanoparticles: Size Matters When Assessing the Immunotoxicity

A Newly Isolated Carboxymethyl-Glucan (CM-G) Restores Depressed Baroreflex Sensitivity in Renovascular Hypertensive Rats

Effects of Baru Almond Oil (Dipteryx alata Vog.) Treatment on Thrombotic Processes, Platelet Aggregation, and Vascular Function in Aorta Arteries

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