Modulator protein RsbR regulates environmental signalling in the general stress pathway of <i>Bacillus subtilis</i>

Akbar, Samina; Kang, Changsun; Gaidenko, Tatiana A.; Price, Chester W.

doi:10.1046/j.1365-2958.1997.3631732.x

Cited by 98 publications

(136 citation statements)

References 43 publications

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“…4B and C). Activity of the B -dependent opuE P-2 promoter rose transiently in response to an osmotic upshock, consistent with the temporal pattern of B activation after sudden environmental challenges (Voelker et al, 1995;Varó n et al, 1996;Akbar et al, 1997). This promoter also allowed the input of other typical B -inducing stimuli (e.g.…”

Section: Discussionmentioning

confidence: 78%

“…2). This pattern of gene expression distinguishes opuE from other members of the B regulon where transcription is only transiently induced after osmotic upshifts (Voelker et al, 1995;Hecker et al, 1996;Varó n et al, 1996;Akbar et al, 1997). It also reflects the physiological role of the OpuE transporter.…”

Section: Discussionmentioning

confidence: 99%

“…The activity of the alternative transcription factor B occurs only transiently subsequent to the imposition of environmental stresses (Varó n et al, 1993;Voelker et al, 1995;Hecker et al, 1996;Akbar et al, 1997). Transcription mediated by opuE P-2 reflects this temporal pattern.…”

Section: Identification Of a Minimal Opue Regulatory Dna Fragmentmentioning

confidence: 99%

“…Its members are thought to prepare B. subtilis cells for various unfavourable circumstances, regardless of the type of stress that was initially responsible for the induction of the B -dependent regulon. A complex network of regulatory proteins allows the integration of both cellular and environmental signals into the control of the activity of the B protein (Voelker et al, 1995;Akbar et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A‐ and sigma B‐dependent stress‐responsive promoters

Spiegelhalter

Bremer

1998

Molecular Microbiology

102

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SummaryThe opuE gene from Bacillus subtilis encodes a transport system (OpuE) for osmoprotective proline uptake and is expressed from two osmoregulated promoters: opuE P-1 recognized by the vegetative sigma factor A ( A ) and opuE P-2 dependent on the stress-induced transcription factor sigma B ( B ). The contributions of these two promoters to osmoregulation of opuE were analysed. Genetic studies using chromosomal opuE-treA operon fusions revealed that opuE transcription is rapidly induced after an osmotic upshock. proportion to the external osmolarity and was maintained at high levels. Moreover, both promoters exhibited a different response to the osmoprotectant glycine betaine in the medium. Our results suggest that at least two different signal transduction pathways operate in B. subtilis to communicate osmotic changes in the environment to the transcription apparatus of the cell.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Identification Of a Minimal Opue Regulatory Dna Fragmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A‐ and sigma B‐dependent stress‐responsive promoters

Spiegelhalter

Bremer

1998

Molecular Microbiology

102

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“…Exposure to physical stress enables RsbT to phosphorylate RsbS. This results in the release of RsbT and its activation of RsbU (Akbar et al, 1997;Chen et al, 2003;Voelker et al, 1995a, b;Yang et al, 1996). RsbS/ RsbT interactions are believed to be modulated by RsbR and a family of related proteins (Akbar et al, 1997(Akbar et al, , 2001Chen et al, 2003;Gaidenko et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Associations between Bacillus subtilis B regulators in cell extracts

Kuo¹

2004

Microbiology

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The general stress regulon of Bacillus subtilis is induced by the activation of the s B transcription factor. Activation of s B occurs as a consequence of the dephosphorylation of its positive regulator RsbV by one of two phosphatases that respond to either physical or nutritional stress. The physical stress phosphatase (RsbU) requires a second protein (RsbT) for activity. Stress is thought to initiate a process that triggers the release of RsbT from a large inhibitory complex composed of multiple copies of two protein species, RsbR (and/or its paralogues) and RsbS. The stress-derived signal driving RsbT release is unknown, but it fails to develop in B. subtilis lacking either ribosome protein L11 or the ribosome-associated protein Obg. RsbR, RsbS, RsbT, Obg and ribosomes elute in common high-molecular-mass fractions during gel-filtration chromatography of crude B. subtilis extracts. This paper reports the investigation of the basis of this coelution by the examining of associations between these proteins in extracts prepared from wild-type and mutant B. subtilis, and Escherichia coli engineered to express RsbR, RsbS and RsbT. Large RsbR/RsbS complexes, distinct from ribosomes, were detected in extracts of both B. subtilis and E. coli. In E. coli, high-molecular-mass forms of RsbS were less abundant when RsbR was absent, but in B. subtilis, only when both RsbR and its principal paralogues were missing from the extract was this form less abundant. This finding is consistent with the notion that the RsbR paralogues, present in B. subtilis but not E. coli, can substitute for RsbR in such complexes. RsbT was not bound to RsbR/RsbS in any extract that was examined, including one prepared from a B. subtilis strain with an RsbS variant (RsbS59SA) that is believed to continuously associate with RsbT. The high-molecular-mass forms of RsbT were found to be Triton-sensitive and independent of any other B. subtilis protein for their formation. These probably represent RsbT aggregates. The data suggest that the contribution of ribosomes/Obg to s B activation does not involve formation of a stable association between these proteins and the Rsb complex. In addition, the binding of RsbT to RsbS/RsbR appears to be more labile than the binding between the previously analysed Rsb proteins which form inhibitory complexes. This, and the apparent proclivity of RsbT to aggregate, suggests an inherent instability in RsbT which may play a role in its regulation.

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From gene regulation to gene function: regulatory networks in Bacillus subtilis

Harwood

Moszer

2002

Comp Funct Genom

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Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. The publication of the B. subtilis genome sequence and subsequent systematic functional analysis and gene regulation programmes, together with an extensive understanding of its biochemistry and physiology, makes this micro-organism a prime candidate in which to model regulatory networks in silico. In this paper we discuss combined molecular biological and bioinformatical approaches that are being developed to model this organism’s responses to changes in its environment.

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Modulator protein RsbR regulates environmental signalling in the general stress pathway of Bacillus subtilis

Cited by 98 publications

References 43 publications

Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A‐ and sigma B‐dependent stress‐responsive promoters

Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A‐ and sigma B‐dependent stress‐responsive promoters

Associations between Bacillus subtilis B regulators in cell extracts

From gene regulation to gene function: regulatory networks in Bacillus subtilis

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