Modulators or facilitators? Roles of lipids in plant root–microbe interactions

Macabuhay, Allene; Arsova, Borjana; Walker, Robert; Johnson, Alexander; Watt, Michelle; Roessner, Ute

doi:10.1016/j.tplants.2021.08.004

Cited by 75 publications

(51 citation statements)

References 85 publications

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“…For example, in spore-treated samples, lipid Classes such as glycerolipids and GPs decreased (leaves) while prenol lipids and sphingolipids increased (roots) (Fig. 4), consistent with their importance in membrane remodeling and signaling during plant-AMF interactions (Wewer et al, 2014;Macabuhay et al, 2022). Interestingly, more sphingolipids showed a decrease in the leaves, but the pattern was reversed in the roots.…”

Section: Compound Class Annotation Reveals An Important Role Of Lipid...supporting

confidence: 70%

Characterization and visualization of global metabolomic responses ofBrachypodium distachyonto environmental changes

Mahood

Bennett

Komatsu

et al. 2022

Preprint

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Plant responses to environmental change are mediated via changes in cellular metabolomes. However, <5% of signals obtained from tandem liquid chromatography mass spectrometry (LC-MS/MS) can be identified, limiting our understanding of how different metabolite classes change under biotic/abiotic stress. To address this challenge, we performed untargeted LC-MS/MS of leaves, roots and other organs of Brachypodium distachyon, a model Poaceae species, under 17 different organ-condition combinations, including copper deficiency, heat stress, low phosphate and arbuscular mycorrhizal symbiosis (AMS). We used a combination of information theory-based metrics and machine learning-based identification of metabolite structural classes to assess metabolomic changes. Both leaf and root metabolomes were significantly affected by the growth medium. Leaf metabolomes were more diverse than root metabolomes, but the latter were more specialized and more responsive to environmental change. We also found that one week of copper deficiency shielded the root metabolome, but not the leaf metabolome, from perturbation due to heat stress. Using a recently published deep learning based method for metabolite class predictions, we analyzed the responsiveness of each metabolite class to environmental change, which revealed significant perturbations of various lipid classes and phenylpropanoids such as cinnamic acids and flavonoids. Co-accumulation analysis further identified condition-specific metabolic biomarkers. Finally, to make these results publicly accessible, we developed a novel visualization platform on the Bioanalytical Resource website, where significantly perturbed metabolic classes can be readily visualized. Overall, our study illustrates how emerging chemoinformatic methods can be applied to reveal novel insights into the dynamic plant metabolome and plant stress adaptation.

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Section: Compound Class Annotation Reveals An Important Role Of Lipid...supporting

confidence: 70%

Characterization and visualization of global metabolomic responses ofBrachypodium distachyonto environmental changes

Mahood

Bennett

Komatsu

et al. 2022

Preprint

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“…We also observed an increase in monoacylglycerols (MAG) levels. MAG comprise the bulk of oil storage in plant tissues and are involved in many regulatory processes such as cell signaling and intracellular trafficking (Macabuhay et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Biostimulant Effects of Chaetomium globosum and Minimedusa polyspora Culture Filtrates on Cichorium intybus Plant: Growth Performance and Metabolomic Traits

et al. 2022

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In this study, we investigated the biostimulant effect of fungal culture filtrates obtained from Chaetomium globosum and Minimedusa polyspora on growth performance and metabolomic traits of chicory (Cichorium intybus) plants. For the first time, we showed that M. polyspora culture filtrate exerts a direct plant growth-promoting effect through an increase of biomass, both in shoots and roots, and of the leaf area. Conversely, no significant effect on morphological traits and biomass yield was observed in C. intybus plants treated with C. globosum culture filtrate. Based on 1H-NMR metabolomics data, differential metabolites and their related metabolic pathways were highlighted. The treatment with C. globosum and M. polyspora culture filtrates stimulated a common response in C. intybus roots involving the synthesis of 3-OH-butyrate through the decrease in the synthesis of fatty acids and sterols, as a mechanism balancing the NADPH/NADP+ ratio. The fungal culture filtrates differently triggered the phenylpropanoid pathway in C. intybus plants: C. globosum culture filtrate increased phenylalanine and chicoric acid in the roots, whereas M. polyspora culture filtrate stimulated an increase of 4-OH-benzoate. Chicoric acid, whose biosynthetic pathway in the chicory plant is putative and still not well known, is a very promising natural compound playing an important role in plant defense. On the contrary, benzoic acids serve as precursors for a wide variety of essential compounds playing crucial roles in plant fitness and defense response activation. To the best of our knowledge, this is the first study that shows the biostimulant effect of C. globosum and M. polyspora culture filtrates on C. intybus growth and metabolome, increasing the knowledge on fungal bioresources for the development of biostimulants.

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“…From the nutritional perspective, ARM was rich in amino acids, nucleotides, and carbohydrates, which provided a different niche from the lipid-rich URE (Fig. 1d) (Koprivova and Kopriva 2022, Macabuhay et al, 2021, Pang et al, 2021, Sasse et al, 2018. Furthermore, the terpenoid-and flavonoid-rich ARM could impose a distinct selective pressure on microbes compared to URE, which contained higher levels of alkaloids and phenolic acids (Fig.…”

Section: Discussionmentioning

confidence: 99%

Microbiota-mediated nitrogen fixation and homeostasis in aerial root-mucilage

Pang

Mao

et al. 2022

Preprint

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Plants sustain intimate relationships with diverse microbes. While it is well recognized that these plant-associated microbiota shape individual performance and fitness of host plants, how they exert their function and maintain their homeostasis are largely unexplored. Here, using the pink lady plant (Heterotis rotundifolia) as a model, we investigated the phenomenon of microbiota-mediated nitrogen fixa-tion as well as the proximal homeostasis mechanisms of this process in the aerial root mucilage. The aerial root mucilage is enriched in primary metabolites such as carbohydrates, and abundant diazo-trophic bacteria. Nitrogen-labeled experiments and gene expression analysis indicate that the aerial root-mucilage microhabitat fixes atmospheric nitrogen to support plant growth. The presence of a key "police" fungus in the mucilage helps the host plant to defend against environmental microbes rather than diazotrophs. The discovery of new biological function and “police” fungi in the aerial root-mucilage microhabitat provides insights into microbial homeostasis maintenance of microenvironmental function and rhizosphere ecology.

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Modulators or facilitators? Roles of lipids in plant root–microbe interactions

Cited by 75 publications

References 85 publications

Characterization and visualization of global metabolomic responses ofBrachypodium distachyonto environmental changes

Characterization and visualization of global metabolomic responses ofBrachypodium distachyonto environmental changes

Biostimulant Effects of Chaetomium globosum and Minimedusa polyspora Culture Filtrates on Cichorium intybus Plant: Growth Performance and Metabolomic Traits

Microbiota-mediated nitrogen fixation and homeostasis in aerial root-mucilage

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