Modulatory effect of Byrsonima verbascifolia (Malpighiaceae) against damage induced by doxorubicin in somatic cells of Drosophila melanogaster

Abstract

ABSTRACT. Byrsonima verbascifolia, popularly known in Brazil as murici, is a medicinal plant widely used in the treatment of bacterial and viral infections, Chagas's disease, diarrhea, bronchitis, cough and fever, as well as for protection of the intestinal mucosa. Since chemotherapy and radiotherapy, broadly employed in the treatment of cancer, can have undesirable side effects, such as inducing DNA damage in normal cells, it would be useful to investigate compounds that inhibit or reduce these effects. A lyophilized water extract of murici, used at three different concentrations (25, 50, and 100 mg/mL), was tested to determine if it could reduce damage induced by the antineoplastic compound doxorubicin in somatic cells of Drosophila melanogaster, analyzed by SMART/wing. The frequency of mutant spots in descendants from standard and high bioactivation crosses was significantly reduced by treatment with murici extract. Further studies are needed using other experimental models, to determine if murici has the potential to be employed by cancer patients receiving chemotherapy.

Vernonanthura polyanthes leaves aqueous extract enhances doxorubicin genotoxicity in somatic cells of Drosophila melanogaster and presents no antifungal activity against Candida spp.

“…In high bioactivation (HB) crossing, 80 female ORR lineage were crossed with 40 mwh males. In both crosses the following offspring was obtained: marked trans-heterozygous (MH) with genetic constitution mwh +/+ flr 3 , which can be easily identified by the smooth wings (Ribeiro et al, 2003(Ribeiro et al, , 2009Valadares, 2007;Mendanha et al, 2010).…”

“…The test is based on the identification of mutant phenotypes that are originated due to the occurrence of DNA damage, caused by the loss of larval heterozygous cells (Fonseca and Pereira, 2004;Ribeiro et al, 2003Ribeiro et al, , 2009Mendanha et al, 2010;Abreu et al, 2011;Alte et al, 2012). The test makes use of three D. melanogaster different strains: (i) multiple wing hairs (mwh); (ii) flare-3 (flr 3 ); and (iii) ORR/ORR.…”

Compostos químicos e aspectos botânicos, etnobotânicos e farmacológicos da Byrsonima verbascifolia Rich ex. A. Juss

“…A. Juss (Malpighiaceae) é popularmente conhecida como "murici" (Morais et al, 2013), "murici-cascudo", "mirici", "douradinha-falsa","muricizinho", "orelha-de-burro", "orelha-de-veado", "semaneira", "baga-de-tucano" (Mendanha et al, 2010). Possui hábito subarbustivo (Barbosa et al, 2005) e folhas grandes e rígidas (Andrade et al, 1995), com pêlos em ambas faces (Andrade et al, 1995;Andrade et al, 1999).…”

Modulatory effects of Duguetia furfuracea (A. St. Hil) Benth. and Hook. f. in Drosophila melanogaster somatic and germinative cells

“…Silva (1998) These results are in accordance with those observed in the present study, which also showed the modulatory effect of D. furfuracea infusion on the damage caused by urethane. Mendanha et al (2010) observed the antigenotoxic activity of the aqueous extract of Byrsonima verbascifolia using the SMART/wing test/wing. This medicinal plant has chemicals that are also present in D. furfuracea, such as flavonoids and terpenes, corroborating the results found in this study.…”