Modulatory effect of heat stress on viability of primary cultured chicken satellite cells and expression of heat shock proteins <i>ex vivo</i>

Siddiqui, Sharif Hasan; Subramaniyan, Sivakumar Allur; Kang, Darae; Park, Jinryong; Khan, Mousumee; Shim, Kwan Seob

doi:10.1080/10495398.2020.1757460

Cited by 14 publications

(10 citation statements)

References 41 publications

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“…As a consequence, this increases the disease susceptibility of broilers (Monson et al 2018), which leads to a high mortality rate (Brossi et al 2018). Moreover, the high temperature reduces the broiler muscle cell proliferation (Siddiqui et al 2020b, Siddiqui et al 2021b) by altering the molecular structure and function of the intestine. Our result is consistent with the results of a previous study, indicating that environmental factors reduce the growth performance of broilers (Zaghari et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Ahmed¹,

Siddiqui

Das

et al. 2022

Preprint

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Broiler meat is known for its nutritional value, availability, and low price. Although, broilers are more sensitive to environmental conditions and need a specific environment for optimum production. However, global warming and natural disasters are important obstacles to broiler production. Therefore, we conducted this study to reveal the broiler production status throughout the year in a subtropical region. In our study, we selected the years 2019, 2020, and 2022. The study area included Noakhali and Lakshimpur, located in the southern part of Bangladesh. We selected 10 smallholder broiler farms following specific conditions and collected information at the end of each month for analysis. The body weight of broilers was significantly lower in July than in other months. However, the mortality was significantly lower in July than in other months of the year. Nonetheless, the prevalence of viral disease was higher in the winter season (January, February, and December). Although, the metabolic disease prevalence was higher in October, a high prevalence of bacterial disease was found in April. These results suggest that high temperatures decrease broiler production while broilers are more susceptible to viral disease in the winter season.

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Section: Discussionmentioning

confidence: 99%

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Ahmed¹,

Siddiqui

Das

et al. 2022

Preprint

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“…This phenomenon was consistent with our previous in vivo study of juvenile spotted sea bass, which showed that the growth performance indicated by the parameters of both specific growth rate (SGR) and weight gain rate (WGR) was significantly higher when reared at 28 °C in comparison with 20 °C after 30 days [ 32 ]. The impacts of temperature on myogenesis have also been studied using a primary cultured muscle cell model in chickens, which reported the significant influence of thermal stress during myogenic proliferation and differentiation stages [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures

Zhang

Wen²,

Qi³

et al. 2022

IJMS

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Temperature strongly modulates muscle development and growth in ectothermic teleosts; however, the underlying mechanisms remain largely unknown. In this study, primary cultures of skeletal muscle cells of Lateolabrax maculatus were conducted and reared at different temperatures (21, 25, and 28 °C) in both the proliferation and differentiation stages. CCK-8, EdU, wound scratch and nuclear fusion index assays revealed that the proliferation, myogenic differentiation, and migration processes of skeletal muscle cells were significantly accelerated as the temperature raises. Based on the GO, GSEA, and WGCNA, higher temperature (28 °C) induced genes involved in HSF1 activation, DNA replication, and ECM organization processes at the proliferation stage, as well as HSF1 activation, calcium activity regulation, myogenic differentiation, and myoblast fusion, and sarcomere assembly processes at the differentiation stage. In contrast, lower temperature (21 °C) increased the expression levels of genes associated with DNA damage, DNA repair and apoptosis processes at the proliferation stage, and cytokine signaling and neutrophil degranulation processes at the differentiation stage. Additionally, we screened several hub genes regulating myogenesis processes. Our results could facilitate the understanding of the regulatory mechanism of temperature on fish skeletal muscle growth and further contribute to utilizing rational management strategies and promoting organism growth and development.

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“…In the present study, we detected a direct proportional influence of TAC-DTE combinations on the total RNA concentration in stressed PBMCs, with RNA concentration decreasing as TACs went from moderate to severe. Previous studies [9,21] showed that in vitro incubation at temperatures simulating hyperthermia subsequently impaired nucleotide synthesis in mitogen-stimulated PBMCs. Moreover, several reports found that RNA as a nucleic acid is heat labile and very unstable and highly denatured under TACs [6].…”

Section: Numbers Ofmentioning

confidence: 99%

“…In normal BT (37°C) conditions, there is an elevation of nucleic acid synthesis due to the introduction of extracellular protein kinase and phosphoinositide 3-kinase/Akt signal transduction during transcription [31,32]. Furthermore, increased cell proliferation, cell viability, and nucleic acid synthesis were shown to happen for longer S and G2/M phases of the cell cycle [7,9]. A recent report also indicated that cells at the G1/S or G2/M checkpoints and in the S phase are more sensitive to death and arrest of proliferation when exposed to TACs [10].…”

Section: Numbers Ofmentioning

confidence: 99%

“…Previous studies [6][7][8][9] reported the effects of variations in thermal assault conditions (TACs) and heat shock on cellular integrity, proliferation, and viability as well as on RNA concentration, thus predisposing animals to vulnerable opportunistic infections resulting from immune response breakdown and decline in production performance and reproduction. RNA as a nucleic acid is heat labile and becomes very unstable and very unstable under harsh environmental conditions, particularly thermal conditions.…”

Section: Introductionmentioning

confidence: 99%

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Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

et al. 2022

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Background and Aim: Global warming has grave consequences on livestock production systems and profound negative effects on animal production. This study aimed to carry out an in vitro thermal stress stimulation (TSS) of bovine peripheral blood mononuclear cells (PBMCs) using different thermal assault conditions (TACs), including normal to extreme temperatures and varying durations of thermal exposure (DTE) to understand how PBMCs of Indian Zebu–Jersey crossbreds respond to various levels and durations of heat shock. Materials and Methods: Ten milliliters of blood were collected from 70 Indian Zebu–Jersey crossbreds under aseptic conditions and were sampled for isolating PBMCs. Peripheral blood mononuclear cells were divided into seven groups, each comprising 10 PBMC samples isolated from 10 different animals. Aliquots of 500 μL of PBMCs were stressed by exposure to different TACs (37, 40, and 45°C) for DTEs of 3 or 6 h. Subsequently, the cells were harvested. The control unstressed samples (500 μL aliquots of PBMCs) were exposed to no TAC (0°C) and zero DTE (0 h). Total RNA from all the treatment groups of PBMCs were isolated and quantitated. Results: We found a very strong association between TACs and RNA levels. In addition, PBMCs viability was negatively affected by heat shock. This led to an exponential reduction in PBMC count as TACs toughened. Only 3.59 × 105 ± 0.34 cells/mL were viable after exposure to 45°C for a 6 h DTE. This cell viability was lower than that measured in controls subjected to no stress and zero time DTE (2.56 × 107 ± 0.22 cells/mL). We also observed a reduction in the concentration of RNA isolated from thermally stressed PBMCs. Conclusion: In vitro TSS of PBMCs provided biological information on the response of cellular systems to heat shock after exposure to TACs. This will help to mitigate and manage the effects of thermal stress in bovine species. The association between the reduction in PBMC count after in vitro TSS and the expression of heat shock protein 70 gene will be investigated in the future to further understand how Indian Zebu–Jersey crossbreds respond to in vitro thermal conditions. This will be used to determine the in vivo response of Indian Jersey crossbreds to different environmental thermal conditions and will further enable the in vivo understanding of thermotolerance potentials of bovine species for better adaptation, survival, and production performance.

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Modulatory effect of heat stress on viability of primary cultured chicken satellite cells and expression of heat shock proteins ex vivo

Cited by 14 publications

References 41 publications

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Growth performance of broilers and prevalence of different diseases vary with environmental variation

Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures

Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds

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