Modulatory effect of selected phenolic acids on enzyme activities of purinergic signaling

Abstract

This research assessed the antioxidant activities and effects of some selected phenolic acids (ferulic, Caffeic, p-coumaric and chlorogenic acid) on enzymes of the purinergic signalling in isolated rat brain which include ATPdase, ecto- 51nucleotidase, phosphodiesterase and Na+/ K+ ATPase. Standard stock solutions (13mM) of the selected phenolics (Caffeic acid, ferulic acid, chlorogenic acid, p-coumaric acid) were prepared and several dilutions (0.05mM-10mM) were subsequently prepared; the ability of the phenolic acids to inhibit Fe2+ induced malondialdehyde (MDA) production in rat’s brain homogenates were assessed. Furthermore, the antioxidant activities of the phenolic acids as typified by the radicals (DPPH* and ABTS*) scavenging abilities were investigated. The results revealed that ferulic acid had the highest Na+/K+ -ATPase (IC50 =3.48 /ml) stimulatory activityas well as the highest ecto-5'-nucleotidase (IC50 =6.222 μg/ml) inhibitory activity while chlorogenic acid had the highest phosphodiesterase-51 (IC50 =21.03μg/ml) inhibitory activity and P-coumaric acid had the highest inhibition of ATPDase (IC50 =17.56 μg/ml) activity. Also, chlorogenic acid had the highest ABTS* and DPPH* scavenging abilities and also the strongest inhibition of Fe2+ induced MDA production in rat brain. However, this study revealed that the phenolic acids altered critical enzymes of the purinergic signaling and therefore suggests their possible neuromodulatory effect.

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“…A recent study [ 53 ] demonstrated that phenolic acids inhibited thiobarbituric acid reactive substances (TBARS) production in a concentration-dependent manner in rat brain homogenate. The compound chlorogenic acid had a higher inhibitory ability (IC 50 = 32.61 μM) compared to that of ferulic acid (IC 50 = 81.38 μM), and a similar trend was found against DPPH radical.…”

“…The compound chlorogenic acid had a higher inhibitory ability (IC 50 = 32.61 μM) compared to that of ferulic acid (IC 50 = 81.38 μM), and a similar trend was found against DPPH radical. Therefore, the mentioned studies [ 51 , 52 , 53 ] lend support on the usefulness of colorimetric methods in the screening of potential biological properties of phenolic bioactives. In addition, a recent contribution improved the DPPH assay to overcome potential interferences of the tested material [ 54 ], also supporting the ongoing importance of colorimetric methods.…”