Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

Piccinno, M.; Rizzo, Annalisa; Maselli, M.A.; DeRosa, M.; Sciorsci, Raffaele Luigi

doi:10.1016/j.theriogenology.2014.08.008

Cited by 12 publications

(15 citation statements)

References 66 publications

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“…Previous studies have shown that various antibiotics not included in the aminoglycoside group, such as clarythromicin (1), erythromicin (37), rifaximin (28,29,32), fosfomycin, amoxicillin and doxycyline (3), ceftiofur (31), enrofloxacin (24, 28, 29) and amoxicillin (28,29), have different effects on uterus contractions. It has been reported that some antibiotics stimulate (28,29,31,32) while some other suppress myometrial contractions (1,24,37). Previous in vitro studies have shown that gentamicin inhibits spontaneous contractions of the isolated uterus in nonpregnant rats (25) and cows (22).…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that rifaximin stimulates uterus contractions in nonpregnant cows (32) and that ceftiofur has an additive effect on the strength of contractile activity of the myometrium, as shown in an in vitro study on nonpregnant heifers Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows and cows (31). In another study, it was reported that amoxicillin inhibits uterus contractions, enrofloxacin increases contraction and rifaximin increases contraction in the follicular phase and causes relaxation in the luteal phase (29). It has been determined that erythromycin inhibits oxytocin and carbacol-induced uterus contractions in pregnant rats (8), and that it also inhibits PGF 2α -induced uterus contractions in nonpregnant rats (2).…”

Section: Praca Oryginalnamentioning

confidence: 99%

See 1 more Smart Citation

Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows

Yüksel¹,

Öcal²,

Ayar³

2019

Medycyna Weterynaryjna

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The aim of the present study is to examine the effects of gentamicin sulphate on spontaneous, oxytocin and PGF2α induced in vitro contractions of myometrium isolated from pregnant cows. Myometrial strips were obtained from healthy pregnant cows and suspended in a covered organ bath filled with Krebs’ solution at 37°C (pH 7.4) continuously bubbled with 95% oxygen and 5% carbon dioxide: isometric contractions were recorded using an isometric force displacement transducer. After the stabilization of spontaneous contractile activity during a 90-minute equilibration period, contractions were recorded for 20 minutes (control). Gentamicin sulphate was then added to the tissue bath cumulatively and the responses were recorded every 20-minutes for each consecutive dose of gentamicin. In agonist-induced contractions, oxytocin or PGF2α was added to the tissue bath at the end of the equilibration period and the same protocol was followed to investigate the effects gentamicin on these agonist-induced contractions. Gentamicin decreased the frequency and inhibited the amplitude of the spontaneous contractions in a dose dependent manner (p < 0.05). The mean frequency and amplitude of oxytocin-induced contractions was significantly inhibited by the application of gentamicin (p < 0.05). Gentamicin also inhibited the contractions induced by PGF2α in a dose dependent manner (p < 0.05). This study showed gentamicin inhibited, depending on the dosage, oxytocin and PGF2α induced contractions of myometrium isolated from pregnant cows. Upon clinically examining the findings obtained by the study, gentamicin can be used as an antibacterial in septic abort and chorioamniotis in order to prevent premature birth, abortion and early uterus contractions. Further studies are necessary to test whether the same effect will take place in vivo and to examine the effects of longterm use of gentamicin on offsprings.

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Section: Resultsmentioning

confidence: 99%

Section: Praca Oryginalnamentioning

confidence: 99%

Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows

Yüksel¹,

Öcal²,

Ayar³

2019

Medycyna Weterynaryjna

View full text Add to dashboard Cite

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“…If an infectious cause remains a possibility following the diagnostic workup for ICI-Pneumonitis, empiric broadspectrum antimicrobial therapy, given in conjunction with immunosuppressive therapy, is advocated (Haanen et al, 2017;Brahmer et al, 2018). Emerging data suggests that the gut microbiome plays a substantial role in the efficacy of ICI-based therapy (Piccinno et al, 2014;Derosa et al, 2018;Zitvogel et al, 2018;Elkrief et al, 2019;Derosa and Zitvogel, 2020). Antibiotics are known to alter the gut microbiome (Zimmermann and Curtis, 2019).…”

Section: Management Of Immune Checkpoint Inhibitor-pneumonitismentioning

confidence: 99%

Pulmonary Toxicities Associated With the Use of Immune Checkpoint Inhibitors: An Update From the Immuno-Oncology Subgroup of the Neutropenia, Infection & Myelosuppression Study Group of the Multinational Association for Supportive Care in Cancer

et al. 2021

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The development of immune checkpoint inhibitors (ICIs) has revolutionized cancer treatment, with agents such as nivolumab, pembrolizumab, and cemiplimab targeting programmed cell death protein-1 (PD-1) and durvalumab, avelumab, and atezolizumab targeting PD-ligand 1 (PD-L1). Ipilimumab targets cytotoxic T lymphocyte-associated antigen-4 (CTLA-4). These inhibitors have shown remarkable efficacy in melanoma, lung cancer, urothelial cancer, and a variety of solid tumors, either as single agents or in combination with other anticancer modalities. Additional indications are continuing to evolve. Checkpoint inhibitors are associated with less toxicity when compared to chemotherapy. These agents enhance the antitumor immune response and produce side- effects known as immune-related adverse events (irAEs). Although the incidence of immune checkpoint inhibitor pneumonitis (ICI-Pneumonitis) is relatively low, this complication is likely to cause the delay or cessation of immunotherapy and, in severe cases, may be associated with treatment-related mortality. The primary mechanism of ICI-Pneumonitis remains unclear, but it is believed to be associated with the immune dysregulation caused by ICIs. The development of irAEs may be related to increased T cell activity against cross-antigens expressed in tumor and normal tissues. Treatment with ICIs is associated with an increased number of activated alveolar T cells and reduced activity of the anti-inflammatory Treg phenotype, leading to dysregulation of T cell activity. This review discusses the pathogenesis of alveolar pneumonitis and the incidence, diagnosis, and clinical management of pulmonary toxicity, as well as the pulmonary complications of ICIs, either as monotherapy or in combination with other anticancer modalities, such as thoracic radiotherapy.

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“…All uteri were found without diseases and so were considered in our study: 28 from cows in the follicular phase and 32 from cows in the luteal phase. The phase of the estrus cycle was identified as reported by Piccinno et al [23]. After cows' slaughter, uteri were collected in about 20 AE 10 minutes.…”

Section: Uterine Strip Preparationmentioning

confidence: 99%

“…Several workers [18,19] demonstrated that oxytocin, when combined with some antibiotics, significantly reduces the formation of bacterial biofilm, but the mechanisms behind this synergistic action are not completely understood. Previous work demonstrates that some antibiotics have intrinsic contractile activity [23]. With this background, the present study was aimed to examine whether antibiotics previously identified as having intrinsic contractile activity could act synergistically with oxytocin in strengthening the contraction activity of bovine uterus.…”

Section: Introductionmentioning

confidence: 99%

Oxytocin plus antibiotics: A synergism of potentiation to enhance bovine uterine contractility

Piccinno

Rizzo

Cariello³

et al. 2016

Theriogenology

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Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

Cited by 12 publications

References 66 publications

Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows

Effects of gentamicin on spontaneous and agonist-induced in vitro contractions of isolated myometrial tissue from pregnant cows

Pulmonary Toxicities Associated With the Use of Immune Checkpoint Inhibitors: An Update From the Immuno-Oncology Subgroup of the Neutropenia, Infection & Myelosuppression Study Group of the Multinational Association for Supportive Care in Cancer

Oxytocin plus antibiotics: A synergism of potentiation to enhance bovine uterine contractility

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