Modulatory influence of Acacia hydaspica R. Parker ethyl acetate extract against cisplatin inveigled hepatic injury and dyslipidemia in rats

Abstract

BackgroundCisplatin (CP) is recommended as a first-line chemotherapeutic agent for solid tumors, however its usage outcomes in severe adverse effects. Acacia hydaspica possesses various phytochemicals and pharmacological activities. The current study aimed to investigate the protective effect of A. hydaspica ethyl acetate extract (AHE) against CP induced aberrations in lipid profile and hepatotoxicity.MethodsRats were randomly separated into six groups (n = 6). Group 1 (control) received distilled water orally for 21 days. Groups 2 (CP control) received a single dose of CP (7.5 mg/kg bw, i.p) on day 16, group 3 (Plant control) received AHE (400 mg/kg b.w, oral) for 21 days, group 4 (post treated group); CP received on day 16 and AHE (400 mg/kg b.w/day, p.o.) was administered after CP till day 21, Group 5 (pretreated group) received AHE (400 mg/kg b.w/day, p.o.) for 21 days and CP (7.5 mg/kg b.w., i.p.) on day 16, group 6 (Silymarin + CP) received 100 mg/kg b.w., p.o. (11 doses/21 days) and CP (7.5 mg/kg b.w., i.p.) on day 16. Lipid profile, liver functional tests, oxidative stress markers, antioxidant enzymes status and histopathological changes were examined.ResultsThe present study revealed that CP caused body weights loss and increase liver index. CP significantly increased serum total lipid, triglycerides and LDL-cholesterol levels. Conversely, it significantly decreased serum HDL-cholesterol level. CP induced marked deteriorations in serum liver function biomarkers, reduced antioxidant enzymes in tissue, while elevated tissue oxidative stress markers along with morphological injuries compared to control rats. Treatment with AHE ameliorated CP induced alterations in lipid profile, serum ALT, AST, ALP and total bilirubin levels and liver weight. Furthermore AHE treatment improved the total protein and antioxidant enzymes levels while decreased the level of MDA, H2O2, and NO. The altered parameters were returned to the control level with AHE pretreatment. Histopathological analysis also supported the biochemical findings. Pretreatment seems to be more effective compared to post treatment indicating protective effect.ConclusionThese results reveal that treatment of AHE may be useful in the prevention of CP induced hepatotoxicity due to its antioxidant potential and polyphenolic constituents.

Acacia hydaspica R. Parker ameliorates cisplatin induced oxidative stress, DNA damage and morphological alterations in rat pulmonary tissue

“…The acute toxicity study was conducted as per the guidelines 425 of Organization for Economic Cooperation and Development (OECD) for testing of chemicals for acute oral toxicity [ 39 ]. The detailed procedure is described in our earlier research [ 32 ]. General behavioral changes were detected by the previously described procedure [ 40 ].…”

“…Various species of genus Acacia were reported for their antioxidant and protective potentials against lung toxicity in animal models [ 31 ]. A. hydaspica AHE fraction showed significant hepato-protective potential against cisplatin persuaded hepatic damage in rats [ 32 ]. Shahid and colleagues reported the protective potential of methanolic extract of Acacia catechu Willd bark.…”

Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

“…Peroxidase (POD) determination The activity level of the POD enzyme assay was performed by following previous protocol [19]. The variation in absorbance of the sample was determined at 470 nm after each 20 s. An alteration in absorbance of 0.01unit/min was determined as one-unit POD level of activity.…”

Antioxidant Traits and Protective Impact of Vorinostat against Cisplatin Induced Hepatoxicity in Rats

“…Oxidative stress and ROS were noted to be involved in CDDP induced hepatic and renal toxicity which is considered to be one of the main causes for liver injury [27]. GSH, is an antioxidant defense enzyme that plays an important role in cellular defense against ROS and cellular damage [28], MDA, is a harmful product of lipid peroxidation produced by oxidative stress causes the damage of cellular membrane integrity and leakage of cellular contents to blood [28]. In the present study, VST prevented CDDP induced increase in rat's serum level of transaminase in combination groups confirming Oxidative stress and ROS were noted to be induced hepatic and renal toxicity which is considered to be one of the main GSH, is an antioxidant defense enzyme that plays an important role in cellular defense against ROS and cellular , MDA, is a harmful product of lipid peroxidation produced by oxidative stress which causes the damage of cellular membrane kage of cellular contents to blood CDDP administration was known to induce oxidative stress in renal epithelial cells and liver cells with depletion of the hepatic GSH and triggered the formation of lipid peroxide These effects were confirmed in the present study by a significant reduction of GSH and an increase of MDA in liver tissues of rats received a single IP dose of CDDP.…”