Moesin and Stress-Induced Phosphoprotein-1 Are Possible Sero-Diagnostic Markers of Psoriasis

Abstract

To identify diagnostic markers for psoriasis vulgaris and psoriatic arthritis, autoantibodies in sera from psoriasis vulgaris and psoriatic arthritis patients were screened by two-dimensional immunoblotting (2D-IB). Based on 2D-IB and MADLI TOF/TOF-MS analyses, eleven proteins each in psoriasis vulgaris and psoriatic arthritis were identified as autoantigens. Furthermore, serum levels of moesin, keratin 17 (K17), annexin A1 (ANXA1), and stress-induced phophoprotein-1 (STIP1), which were detected as autoantigens, were studied by dot blot analysis with psoriasis patients and healthy controls. The levels of moesin and STIP1 were significantly higher in sera from patients with psoriasis vulgaris than in the controls (moesin: P<0.05, STIP1: P<0.005). The area under the curve (AUC) for moesin and STIP1 between patients with psoraisis vulgaris and controls was 0.747 and 0.792, respectively. STIP1 and K17 levels were significantly higher in sera from patients with psoriatic arthritis than in those with psoriasis vulgaris (P<0.05 each). The AUC for STIP1 and K17 between patients with psoriatic arthritis and psoriasis vulgaris was 0.69 and 0.72, respectively. The STIP1 or moesin, CK17 serum level was not correlated with disease activity of psoriasis patients. These data suggest that STIP1 and moesin may be novel and differential sero-diagnostic markers for psoriasis vulgaris and psoriatic arthritis.

A systematic review of diagnostic criteria for psoriasis in adults and children: evidence from studies with a primary aim to develop or validate diagnostic criteria

“…Within the 11 702 studies identified from the search strategy no clinical examination‐based diagnostic criteria for psoriasis in adults or children were found. Only 23 studies met the inclusion criteria; they presented a broad range of diagnostic criteria including genetic, molecular, dermoscopy, confocal microscopy, histopathology, questionnaire‐based, computer‐aided and traditional Chinese medicine criteria (Table S1; see Supporting Information). Sixteen studies were of a case–control design, five studies were case series, one study was cross‐sectional and one study was a Delphi consensus study.…”

“…The data labels on the scatter plot relate to the references within the review. For Maejima et al . the moesin data are presented.…”

“…Seven studies were of a case–control design and one was a cross‐sectional study. Sensitivity and specificity results of the criteria were presented or available on request for five studies; the sensitivity values ranged from 65·6% to 98% and specificity from 58·2% to 100% . Diagnostic accuracy results were often reported as area under the curve, and three studies reported a value ≥0·7 .…”

“…Diagnostic accuracy results were often reported as area under the curve, and three studies reported a value ≥0·7 . Six studies undertook validation testing of their developed criteria; in two studies this was conducted in a separate cohort, but no studies reported validation in their intended population.…”

Proteomic analysis of physiological versus pathological cardiac remodeling in animal models expressing mutations in myosin essential light chains

“…Moesin is an important cytoskeletal protein which has been shown to interact with ezrin and radixin (Amsellem et al 2014). Interestingly, meosin and stress-induced phosphoprotein-1, which were both downregulated in Δ43 hearts and not in A57G hearts, have been recently suggested to be possible sero-diagnostic markers of psoriasis (Maejima et al 2014). Among cytoskeletal proteins altered in pathologic (A57G) versus physiologic (Δ43) hypertrophy was myosin heavy chain 6 (α-MHC) downregulated in A57G hearts (Table 5).…”