Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production

Nascimento, Miguel Figueiredo; Barreiros, Ricardo; Oliveira, Ana; Ferreira, Frederico Castelo; Faria, Nuno Torres

doi:10.1007/s13399-022-02837-y

Cited by 14 publications

(20 citation statements)

References 43 publications

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“…Mannosylerythritol lipids (MELs) used in this study were produced by Moesziomyces antarcticus PYCC 5048 T (CBS 5955) and M. aphidis PYCC 5535 T , provided by the Portuguese Yeast Culture Collection (PYCC), CREM, FCT/UNL, Caparica, Portugal, following previously established fermentation medium and conditions [ 19 ]. Yeasts were cultivated in a 2-L bioreactor (New Brunswick TM Bioflo R /CelliGen R 115), using 1.5 L of working volume, with a medium composition described elsewhere [ 32 ]. The temperature was set up at 27 °C and the pH was not controlled.…”

Section: Methodsmentioning

confidence: 99%

Novel Organic Solvent Nanofiltration Approaches for Microbial Biosurfactants Downstream Processing

et al. 2023

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Glycolipid biosurfactants are the most prominent group of microbial biosurfactants, comprising rhamnolipids, sophorolipids and mannosylerythritol lipids (MELs). Usually, large amounts of hydrophobic substrates (e.g., vegetable oils) are used to achieve high titers (~200 g/L) of a crude product of low purity at values limited to 50–60%, contaminated with unconsumed triacylglycerol and residual free fatty acids and monoacylglycerides. The methods reported for the removal of these contaminants use a mixture of organic solvents, compromising solvent recyclability and increasing final process costs. This study reports, for the first time, an innovative downstream method for MELs, in which 90% of the triacylglycerols are separated from the crude MEL mixture in a first stage and the other lipid derivatives (free fatty acids, mono- and diacylglycerols) are removed by organic solvent nanofiltration (OSN). Three commercially available membranes (GMT-oNF-2, PuraMEm-600 and DuramMem-500) and several homemade membranes, casted from 22, 24 or 26% (w/v) polybenzimidazole (PBI) solutions, were assessed for crude MELs purification by diafiltration. A final purity of 87–90% in the MELs was obtained by filtering two diavolumes of methanol or ethyl acetate solutions through a PBI 26% membrane, resulting in MELs losses of 14.7 ± 6.1% and 15.3 ± 2.2%, respectively. Higher biosurfactant purities can be archived using the PBI 26% membrane at higher DV, but at the cost of higher product losses. Namely, in MeOH, the use of 6 DV leads to losses of 32% for MELs and 18% for sophorolipids. To obtain MELs at reagent grade with purities equal or higher than 97%, a two-sequential cascade filtration approach was implemented using the commercial membrane, GMT-oNF. In such a process, MELs with 98% purity was obtained at the cost of 11.6% MELs losses. Finally, decoloration, important in some applications, was successfully assessed using activated carbon. Overall, this study reports a unique solution for microbial biosurfactants production with minimal product losses, enabling solvent recycling and potentially reducing costs.

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Section: Methodsmentioning

confidence: 99%

Novel Organic Solvent Nanofiltration Approaches for Microbial Biosurfactants Downstream Processing

et al. 2023

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“…A study by (Nascimento et al, 2023) methods can be challenging due to the interference of biomass with downstream separation processes-biomass will also be collected along with precipitated MELs. Consequently, additional cell separation techniques may be required before implementing an in situ precipitation process (Teke, Tai, et al, 2022).…”

Section: Membrane Filtrationmentioning

confidence: 99%

“…These studies led to a good understanding of the optimal conditions for labscale MEL production in shake flasks. It has been observed that vegetable oils, most notably soybean oil, serve as the optimal carbon F I G U R E 6 An illustration of the proposed downstream process which integrates solvent selective dissolution and organic solvent nanofiltration (OSN) for the purification of mannosylerythritol lipids modified from Nascimento et al (2023).…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Bhangale et al (2013) produced MELs at a concentration of 5.61 g/L by growing P. antarctica ATCC 32657 on 14% (w/v) honey, whileMadihalli et al (2020) produced MELs at a concentration of 3.85 g/L by growing P. antarctica JCM 10317 on coconut water.Finally,Andrade et al (2017) produced MELs by growing P. tsukubaensis on cassava wastewater in a 3 L bioreactor. Finally, recent research has been aimed at the production of MEL from a combination of hydrophobic and hydrophilic waste streams Nascimento et al (2022). successfully produced MELs at a concentration of 13.98 and 13.1 g/L by cultivating P. antarctica PYCC 5048 and P. aphidis PYCC 5535 on a combination of 30% (v/v) cheese whey and 20 g/L waste cooking oil(Nascimento et al, 2022).Similarly,Faria et al (2023) investigated the production of MELs by P. antarctica PYCC 5048 and P. aphidis PYCC 5535, initially grown on 40 g/L glucose before being supplemented with 20 g/L waste cooking oil.…”

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A review on the upstream production and downstream purification of mannosylerythritol lipids

Valkenburg,

Ncube,

Teke

et al. 2023

Biotech & Bioengineering

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Biosurfactants are natural compounds with remarkable surface‐active properties that may offer an eco‐friendly alternative to conventional surfactants. Among them, mannosylerythritol lipids (MELs) stand out as an intriguing example of a glycolipid biosurfactant. MELs have been used in a variety of sectors for various applications, and are currently commercially produced. Industrially, they are used in the pharmaceutical, cosmetic, food, and agricultural industries, based on their ability to reduce surface tension and enhance emulsification. However, despite their utility, their production is comparatively limited industrially. From a bioprocessing standpoint, two areas of interest to improve the production process are upstream production and downstream (separation and purification) product recovery. The former has seen a significant amount of research, with researchers investigating several production factors: the microbial species or strain employed, the producing media composition, and the production strategy implemented. Improvement and optimization of these are key to scale‐up the production of MELs. On the other hand, the latter has seen comparatively limited work presented in the literature. For the most part traditional separation techniques have been employed. This systematic review presents the production and purification methodologies used by researchers by comprehensively analyzing the current state‐of‐the‐art with regards the production, separation, and purification of MELs. By doing so, the review presents different possible approaches, and highlights some potential areas for future work by identifying opportunities for the commercialization of MELs.

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“…Pre-treated lignocellulosic residues by an enzymatic process were fed to M. antarcticus PYCC 5048 T and M. aphidis PYCC 5535 T to produce MEL [23]. Other hydrophilic sources have been recently assessed, such as the use of coconut water [24], cassava wastewater [25], or cheese whey [26]. While these studies using hydrophilic carbon sources report low accumulation of residual lipids, the titres obtained are still low.…”

Section: Introductionmentioning

confidence: 99%

Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp.

Faria

Nascimento

Ferreira

et al. 2023

Appl Biochem Biotechnol

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Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, their large-scale production and application are restricted by high production costs. Such costs can be reduced using renewable raw materials and facilitated downstream processing. Here, a novel strategy for mannosylerythritol lipid (MEL) production explores the combination of hydrophilic and hydrophobic carbon sources sideways with a novel downstream processing strategy, based on nanofiltration technology. Co-substrate MEL production by Moesziomyces antarcticus was threefold higher than using D-glucose with low levels of residual lipids. The use of waste frying oil instead of soybean oil (SBO) in co-substrate strategy resulted in similar MEL production. Moesziomyces antarcticus cultivations, using 3.9 M of total carbon in substrates, yields 7.3, 18.1, and 20.1 g/L of MEL, and 2.1, 10.0, and 5.1 g/L of residual lipids, for D-glucose, SBO, and a combination of D-Glucose and SBO, respectively. Such approach makes it possible to reduce the amount of oil used, offset by the equivalent molar increase in D-glucose, improving sustainability and decreasing residual unconsumed oil substrates, facilitating downstream processing. Moesziomyces spp. also produces lipases that broken down the oil and, thus, residual unconsumed oils are in the form of free fatty-acids or monoacylglycerol, which are smaller molecules than MEL. Therefore, nanofiltration of ethyl acetate extracts from co-substrate-based culture broths allows to improve MEL purity (ratio of MEL per total MEL and residual lipids) from 66 to 93% using 3-diavolumes.

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Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production

Cited by 14 publications

References 43 publications

Novel Organic Solvent Nanofiltration Approaches for Microbial Biosurfactants Downstream Processing

Novel Organic Solvent Nanofiltration Approaches for Microbial Biosurfactants Downstream Processing

A review on the upstream production and downstream purification of mannosylerythritol lipids

Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp.

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