Mogroside V Protects Porcine Oocytes From Lipopolysaccharide-Induced Meiotic Defects

Abstract

Accumulating evidence has demonstrated that lipopolysaccharide (LPS) compromises female reproduction, especially oocyte maturation and competence. However, methods to protect oocyte quality from LPS-induced deterioration remain largely unexplored. We previously found that mogroside V (MV) can promote oocyte maturation and embryonic development. However, whether MV can alleviate the adverse effects of LPS exposure on oocyte maturation is unclear. Thus, in this study, we used porcine oocytes as a model to explore the effects of MV administration on LPS-induced oocyte meiotic defects. Our findings show that supplementation with MV protected oocytes from the LPS-mediated reduction in the meiotic maturation rate and the subsequent blastocyst formation rate. In addition, MV alleviated the abnormalities in spindle formation and chromosome alignment, decrease in α-tubulin acetylation levels, the disruption of actin polymerization, and the reductions in mitochondrial contents and lipid droplet contents caused by LPS exposure. Meanwhile, LPS reduced m6A levels in oocytes, but MV restored these epigenetic modifications. Furthermore, MV reduced reactive oxygen species (ROS) levels and early apoptosis in oocytes exposed to LPS. In summary, our study demonstrates that MV can protect oocytes from LPS-induced meiotic defects in part by reducing oxidative stress and maintaining m6A levels.

Mogroside V Alleviates Oocyte Meiotic Defects and Quality Deterioration in Benzo(a)pyrene-Exposed Mice

“…As expected, we further found that MV treatment improved the proportion of 2-cell embryos and blastocysts in BaP-exposed mice. In addition, previous studies have demonstrated that MV can improve porcine oocyte in vitro maturation and protect oocytes from quality deterioration induced by postovulatory ageing or LPS exposure (Nie et al, 2019;Nie et al, 2020;Yan et al, 2021). As subsequent embryonic development potential is an indicator of oocyte cytoplasmic maturation, our findings show that MV might alleviate the deterioration of oocyte cytoplasmic maturation induced by BaP exposure.…”

“…In vitro studies have demonstrated that MV can alleviate lipopolysaccharide-induced inflammation in cultured cells (Li et al, 2019;Liu et al, 2021). Moreover, we found that MV supplementation in vitro maturation medium can promote porcine oocyte maturation (Nie et al, 2020), alleviate the quality deterioration of in vitro ageing oocytes (Nie et al, 2019), and protect porcine oocytes from meiotic defects induced by LPS (Yan et al, 2021). Due to its beneficial effects on oocyte maturation and competence, we speculate that MV can protect oocytes from meiotic defects caused by BaP exposure.…”

“…We found that spindle assembly and chromosome alignment were disrupted by BaP exposure and associated with decreased acetylation levels at α-tubulin. However, MV could notedly alleviate the defects of cytoskeletal structures in BaPexposed oocytes, further explaining the ways MV ameliorates the decline of oocyte nucleic maturation (Yan et al, 2021).…”

“…A previous study revealed that melatonin and ginsenoside compound K can attenuate oxidative stress to protect porcine oocytes from meiotic defects caused by BaP exposure (Miao et al, 2018;Luo et al, 2020). In addition, we recently reported that MV inhibits the increase in ROS levels in porcine oocytes exposed to LPS (Yan et al, 2021). Oxidative stress results in diverse adverse changes, such as DNA damage and apoptosis in oocytes, which deteriorate oocyte quality and fertilization capacity (Tamura et al, 2008;Collins et al, 2015).…”

“…MV is an excellent antioxidant with a very strong scavenging effect on ROS in cultured cells and tissues (Zhang X. et al, 2018;Liu et al, 2018). In addition, MV can also reduce excessive ROS accumulation in oocytes during in vitro maturation and the postovulatory ageing process or in LPS-exposed oocytes (Nie et al, 2019;Nie et al, 2020;Yan et al, 2021). Relatively low ROS levels prevent oocytes from impairments induced by oxidative stress.…”