The use of wood contributes to the global transformation into a bio-based community. There are, however, challenges. The growth of mold, rot fungi, and algae on the coated surface is of major concern due to decreased aesthetic service life and shorter maintenance intervals. The colonization of a coated surface requires the presence of spores, nutrients, and a sufficient amount of water. This work studied the influence of using heartwood and sapwood on the moisture content (MC) and growth of microorganisms on the surface of coated Norway spruce boards [Picea abies (L.) Karst.]. The results revealed a relationship of heartwood samples having a lower MC and a lower or equal degree of biological growth on the coated surface than sapwood samples. The relationship was valid through a range of densities (309-548 kg/m 3 ) and two different coating systems based on either an alkyd or an acrylic resin. Furthermore, the choice of coating color (red compared to white) affected the MC as the red-colored samples had a lower MC, combined with no growth of microorganisms.