Moisture content of vital vs endodontically treated teeth

Papa, J.; Cain, C; Messer, Harold H.

doi:10.1111/j.1600-9657.1994.tb00067.x

Cited by 147 publications

(103 citation statements)

References 8 publications

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“…However, in this study, inter-individual variabilities were high, also giving higher values compared to the results of this study. Additionally, all bound water was not regarded by Papa and others, 25 which might explain the marginally lower values compared to the values from this study. As mentioned above, human dentin contains approximately 11% water, but the individual values range between 8% and 16%, depending on methodical differences.…”

Section: Discussioncontrasting

confidence: 55%

“…The overall water content of bovine dentin obtained with the applied Karl-Fischer-methodology was 15.2%. This value is slightly higher than the average outcome by Papa and others, 25 who investigated the water content of human dentin thermogravimetrically. However, in this study, inter-individual variabilities were high, also giving higher values compared to the results of this study.…”

Section: Discussioncontrasting

confidence: 40%

“…In the literature, the free water content of dentin was mostly investigated by determining the weight loss gravimetrically. 12,14,[24][25] There are different approaches to investigating free and bound water fractions by Differential Thermal Analysis [DTA] 26 or quantitative infrared spectroscopical analysis. 27 A disadvantage to the DTA and spectroscopic methods is the qualitative changes that occur in the content of bound water.…”

Section: Discussionmentioning

confidence: 99%

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Influence of Bleaching Agents and Desensitizing Varnishes on the Water Content of Dentin

Betke

Kahler

Reitz

et al. 2006

Operative Dentistry

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The dehydration of tooth structure is discussed as a possible factor for tooth hypersensitivity arising during bleaching; however, it still remains unclear to what extent glycerinebased bleaching gels dehydrate dentin and whether dentin hydration can be maintained by protective dentin varnishes. SUMMARYThis in vitro study investigated the possible dehydration of dentin caused by bleaching agents. Furthermore, it tested whether protective dentin varnishes can maintain the physiological moisture of dentin during bleaching treatment. Fifty-five standardized dentin cylinders were prepared from freshly extracted bovine incisors under constant water irrigation. Prior to bleaching, the treatment specimens were conditioned at room temperature in a hygrophor for 14 days. The samples were divided into 11 groups. The Group A specimens, which were completely dehydrated, and Group B, which was stored for 2 weeks in a hygrophor, served as controls (A, B n=5). The other samples (n=10 each group) were coated with Vivasens [VS] (C), Bilfuorid [BF] (D) and Seal&Protect [SP] (E). Five specimens from each group (C-E) were subsequently treated with an experimental bleaching gel (Exp BG) (20% carbamide peroxide [CP], glycerine-based gel): Cb, Db, Eb. The remaining specimens were bleached with Exp BG (F) only, Vivastyle (G: 16% CP, glycerine-based gel) or Vivastyle Paint On (H: 6% CPvarnish) for 7 days (n=5 each group) with bleaching time for gels: 2 hours/day, paint on: 20 minutes/day. After the respective treatments, the overall water content of each specimen was determined using the analytical method of KarlFischer-titration. The water content of bovine dentin (Group B, mean%±SD) obtained in this

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Section: Discussioncontrasting

confidence: 55%

Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

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Influence of Bleaching Agents and Desensitizing Varnishes on the Water Content of Dentin

Betke

Kahler

Reitz

et al. 2006

Operative Dentistry

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“…12 However, even necrotic teeth contain dentinal fluid. 13 Using the cellulose membrane technique described here, we were able to collect MMPs from teeth with necrotic teeth also (data not shown). Consequently, absorption of fluid through dentin by cellulose may be necessary to get maximum yields regardless of the condition of the pulp.…”

Section: Discussionmentioning

confidence: 99%

Comparison of vehicles to collect dentinal fluid for molecular analysis

Zehnder

Rechenberg

Bostancı

et al. 2014

Journal of Dentistry

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OBJECTIVES To test the hypothesis that a material with higher water absorption than polyvinylidene fluoride (PVDF) could increase the yield of target molecules from exposed dentine. METH-ODS In a series of standard tests, different cellulose membranes were compared to a PVDF counterpart for their ability to absorb water and release protein. In a subsequent randomized clinical trial, the cellulose material with the most favourable values was compared to PVDF regarding the levels of MMP-2 that could be collected from exposed dentine of healthy human teeth during filling replacement. MMP-2 levels were determined by enzyme-linked immunosorbent assay (ELISA). Data from the laboratory experiments were compared between materials using the appropriate parametric tests. The frequency of cases yielding quantifiable levels of MMP-2 was compared between materials by Fisher's exact test. The level of significance was set at 5%. RESULTS The cellulose membrane with the largest pore size (12-15 m) absorbed significantly (P<0.05) more water than PVDF. It showed a protein release that was similar to that of PVDF, while the cellulose membranes with smaller pore size retained significantly more protein (P<0.05). Using the large-pore cellulose membrane, MMP-2 could be collected at a quantifiable level from the dentine of healthy teeth in 9 of 13 cases, compared to 1 of 13 with the PVDF membrane (P<0.05). CONCLUSIONS Under the current conditions, a large-pore cellulose membrane yielded more of a molecule of diagnostic value compared to a standard PVDF membrane. CLINICAL SIGNIFICANCE Molecular diagnostics of dentinal fluid are hampered by low yields. In the current study, it was shown that cellulose membranes are more useful to collect MMP-2 from dentinal fluid than PVDF membranes. ABSTRACTObjectives: To test the hypothesis that a material with higher water absorption than polyvinylidene fluoride (PVDF) could increase the yield of target molecules from exposed dentin. Methods:In a series of standard tests, different cellulose membranes were compared to a PVDF counterpart for their ability to absorb water and release protein. In a subsequent randomized clinical trial, the cellulose material with the most favorable values was compared to PVDF regarding the levels of MMP-2 that could be collected from exposed dentin of healthy human teeth during filling replacement. MMP-2 levels were determined by enzyme-linked immunosorbent assay (ELISA). Data from the laboratory experiments were compared between materials using the appropriate parametric tests. The frequency of cases yielding quantifiable levels of MMP-2 was compared between materials by Fisher's exact test. The level of significance was set at 5%. Results:The cellulose membrane with the largest pore size (12 -15 µm) absorbed significantly (P < 0.05) more water than PVDF. It showed a protein release that was similar to that of PVDF, while the cellulose membranes with smaller pore size retained significantly more protein (P < 0.05). Using the large-pore cellulose membrane, MM...

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“…22 In the present study, the use of different rewetting solutions simulated the conditions of dentin moisture. Under in vivo conditions, the root dentin of endodontically treated teeth has a moisture level of 12.1%, 23 and this moisture is perhaps insufficient to promote the bioactivity of materials within the root canal. Reys-Carmona et al 24,25 suggested the use of PBS as intracanal medication to promote biomineralization of MTA apical plugs in the interior portion of root canals, since the biomineralization of MTA can only be achieved in direct contact with the periodontium and dentin.…”

Section: Discussionmentioning

confidence: 99%

Effect of rewetting solutions on micropush-out dentin bond strength of new bioceramic endodontic material

et al. 2017

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To evaluate the influence of rewetting solutions on bond strength to root dentin of conventional gutta-percha (GP) or niobium phosphate glass-based gutta-percha (GNb) associated with a bioceramic sealer. The root canals of 80 human mandibular premolars were prepared using nickel-titanium instruments and irrigation with sodium hypochlorite and EDTA. The teeth were randomly divided into four groups according to the gutta-percha used: GNb or GP associated with EndoSequence BC Sealer (BC) and the solution for rewetting dentin before filling (distilled water; phosphate buffer saline solution -PBS; simulated body fluid -SBF; or no solution). The root canals were filled with a single cone using warm vertical condensation. Micropush-out bond strengths associated with the filling materials in slices from middle root thirds was determined 30 days after root filling. The failure mode was analyzed with stereoscopic lens. The data were statistically analyzed by two-way ANOVA and Holm-Sidak test (p < 0.05). There was significant difference in the types of gutta-percha (p < 0.001) and in the different rewetting solutions (p = 0.003). The interaction between gutta-percha and rewetting solutions was not significant (p = 0.53). The SBF solution provided an increase in bond strength for both gutta-percha solutions. The GNb+BC (3.42 MPa) association increased bond strength when compared with GP+BC (2.0 MPa). The use of SBF as a dentin rewetting solution increased bond strength in the groups studied. Association of GNb with bioceramic sealer was beneficial, increasing the bond strength to dentin when compared with the association with GP.

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Moisture content of vital vs endodontically treated teeth

Cited by 147 publications

References 8 publications

Influence of Bleaching Agents and Desensitizing Varnishes on the Water Content of Dentin

Influence of Bleaching Agents and Desensitizing Varnishes on the Water Content of Dentin

Comparison of vehicles to collect dentinal fluid for molecular analysis

Effect of rewetting solutions on micropush-out dentin bond strength of new bioceramic endodontic material

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