1The wide range of optimal values reported for the physical parameters of compost 2 mixtures suggest that their interactive relationships should be investigated. The objective of this 3 study was to examine the microbial O 2 uptake rate (OUR) in 16 sludge waste recipes, offering a 4 range of moisture content (MC), waste/bulking agent (W/BA) ratio and BA particle size levels 5 determined using a central composite experimental design. The 3 kg samples were maintained at 6 a constant temperature and aeration rate for 28 days, during which a respirometer recorded O 2 7 uptake to provide a measure of microbial activity and biodegradability. The cumulative O 2 8 consumption after 14 and 28 days was found to be significantly influenced by MC, W/BA ratio, 9BA particle size and the interaction between MC and W/BA ratio (p < 0.05). Using multivariate 10 regression analysis, the experimental data was used to generate a model with good predictive 11 ability for cumulative O 2 consumption after 28 days as a function of the significant physical 12 variables (R 2 = 0.84). The prediction of O 2 uptake by the model depended highly on the 13 interaction between MC and W/BA ratio. A MC outside of the traditional 50-60 % (wet basis) 14 range still resulted in a high level of microbial O 2 uptake as long as the W/BA ratio was adjusted 15 to maintain a good O 2 exchange in the sample. The evolution of OUR in the samples was also 16 investigated, uncovering strong associations between short and long-term respirometric indices, 17 such as peak OUR and cumulative O 2 consumption (p < 0.005). Combining peak OUR data with 18 cumulative O 2 consumption after 14 days allowed for accurate predictions of cumulative O 2 after 19 28 days of aeration (R 2 = 0.96), implying that future studies need only run trials up to 14 days to 20 evaluate the overall O 2 consumption or biodegradability of a sludge mixture. 21