Molded bone augmentation by a combination of barrier membrane and recombinant human bone morphogenetic protein‐2

Takeishi, Hideyuki; Irie, Kazuharu; Okuda, K; Ozawa, Hiroki; Yajima, Toshihiko; Ejiri, Sadakazu

doi:10.1034/j.1601-0825.2001.00725.x

Cited by 16 publications

(10 citation statements)

References 24 publications

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“…Previously, we showed that rhBMP-2-induced domeshaped areas of bone underwent remodeling that resulted in a decrease of height [24]. The present study demonstrated that the dome-shaped bone contained a large amount of OPN, and had been slowly replaced by highly mineralized mature bone during remodeling.…”

Section: Discussionmentioning

confidence: 52%

Changes of Osteopontin Distribution and Matrix Mineralization during Remodeling in Experimental Bone Formation.

Irie

Takeishi

Tsuruga

et al. 2002

Acta Histochem. Cytochem

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When bone is rapidly induced by recombinant human bone morphogenetic protein-2 (rhBMP-2), more noncollagenous proteins (NCPs) are accommodated among the collagen meshwork. These NCPs are then removed, resulting in highly mineralized mature bone. However, few reports have focused on changes in the bone matrix of rhBMP-2-induced bone. In the present study, rhBMP-2 with an artificial carrier was implanted over bone defects in rat calvariae, and changes in the distribution of osteopontin (OPN), the degree of mineralization and speed of bone formation were investigated histochemically and radiographically. Domeshaped areas of newly formed bone observed at postoperative week 2 were intensely immunoreactive for OPN and intensely labeled with calcein, but were not as radiopaque as the preexisting bone. At postoperative week 8, intense immunoreactivity was detected only on cement lines and in small discrete areas on the flattened domes. The matrix was as radiopaque as, and indistinguishable from, the preexisting bone. Only thin linear labeling of calcein was found on the bone surface. These findings suggest that, in rhBMP-2 induced bone, production of OPN is increased when the rate of bone formation is high, and that OPN produced in the early stage of bone formation is removed during bone remodeling to create a highly mineralized mature bone matrix.

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Section: Discussionmentioning

confidence: 52%

Changes of Osteopontin Distribution and Matrix Mineralization during Remodeling in Experimental Bone Formation.

Irie

Takeishi

Tsuruga

et al. 2002

Acta Histochem. Cytochem

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“…Therefore, the use of a membrane barrier to reduce osteoclastic bone graft resorption is a good alternative if there is no time to harvest the bone and to preserve the periosteum during surgery. Several studies [47,48] have used mineralizing substances to promote bone regeneration under the resorbable collagen membranes, and substances that promote bone regeneration have also been reported. Hence, some good results are expected.…”

Section: Discussionmentioning

confidence: 99%

A comparison study between periosteum and resorbable collagen membrane on iliac block bone graft resorption in the rabbit calvarium

et al. 2014

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BackgroundTo compare the different resorption patterns between resorbable membrane barrier and periosteum after iliac block bone grafting radiographically and histologically.MethodsEighteen mature male rabbits weighing from 2.0 to 2.5 kg were used. The recipient site was the rabbit skull, and autogenous iliac bone was used as the grafting material. The harvested iliac block bones were divided in the following groups: autogenous iliac block bone with preservation of the periosteum (the periosteum group), autogenous iliac block bone covered with a resorbable collagen membrane (Biomesh®, Samyang Co, Korea) after removing the periosteum (the collagen membrane group), and autogenous iliac block bones with removal of the periosteum (the control group). In each experimental group, periosteum or resorbable collagen membrane of the donor site was fixed directed to the periosteum of the recipient site. The specimens were examined macroscopically, radiographically, histologically, and histomorphometrically at every 2, 4, and 8 weeks.ResultsAll groups presented excellent bone graft healing state without inflammation, dehiscence, or displacement. The radiolucency increased from mild to moderate in all groups over the experiment. The mean thickness of the upper end of the cortical iliac bone graft was statistically significantly different between the control group and the periosteum group, between the four-week and eight-week control group, and between the four- week and eight-week periosteum group (p & 0.05).ConclusionThis study suggests that both the periosteum and the resorbable collagen membrane may help to prevent soft tissue infiltration into the bone graft and to reduce bone graft resorption compared to block graft alone.

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“…Critical size defects in cranium, mandible, femur and other portions of the rat skull are often used as experimental models to test bone repair materials [10][11][12]. A calvaria defect model has many similarities to defects in maxillofacial region.…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, many studies use type-I collagen, osteocalcin and osteopontin antibodies as markers for osteoblasts [10,18,19]. In our study, we wanted to use multiple ways, such as gross finding, radiographs quantitative radiograph analysis and histology methods, to evaluate the capacity of bone repair of DG compared with that of BG.…”

Section: Discussionmentioning

confidence: 99%

Application of a Novel Resorbable Membrane in the Treatment of Calvarial Defects in Rats

Feng

Wang

2011

Journal of Biomaterials Science, Polymer Edition

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Diplen-Gam (DG) is a novel absorbable guided bone regeneration (GBR) membrane. This study was designed to evaluate the capacity of bone repair of DG compared with that of Bio-Gide (BG). Critical size defects were created in both sides of the calcarium of 36 Sprague-Dawley rats. Defects were assigned to six groups and each group was subjected to one of the following treatments: (A1) unfilled defects, (A2) Bio-Oss (BO) grafts, (B1) DG membrane, (B2) BG membrane, (C1) DG membrane + BO grafts and (C2) BG membrane + BO grafts. The animals were killed at 2, 4, 8 and 12 weeks after the operation. The defects and surrounding tissues were examined by gross observation and X-ray examination. The paraffin sections were subjected to HE (hematoxylin and eosin) staining and IHC (immunohistochemistry) for bone morphogenetic protein-2 (BMP-2). The X-rays showed that, at 12 weeks, the DG and BG group exhibited more new bone formation than CSD blank group did; the BG group exhibited more new bone formation than the DG group did (t = 5.240, P = 0.035), the BG + BO group showed no significant differences in bone formation compared with the DG + BO group (t = 1.246, P = 0.339). By IHC staining, BMP-2-positive results could be seen inside the DG membrane, on the surface of the new bone, and inside the new bone. It can be suggested that BG membrane achieved better effects in guided bone regeneration compared with DG membrane. No significant differences were found between the two membranes in their bone healing ability when they are used with BO. Therefore, DG membrane shows clinical effectiveness, but should be used in combination with bone substitute.

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Molded bone augmentation by a combination of barrier membrane and recombinant human bone morphogenetic protein‐2

Abstract: The results suggest that the combination of rhBMP-2 and barrier membrane has advantages in producing and maintaining bone in the intended shape by inducing osteoblasts directly on the inner surface of the membrane.

Cited by 16 publications

References 24 publications

Changes of Osteopontin Distribution and Matrix Mineralization during Remodeling in Experimental Bone Formation.

Changes of Osteopontin Distribution and Matrix Mineralization during Remodeling in Experimental Bone Formation.

A comparison study between periosteum and resorbable collagen membrane on iliac block bone graft resorption in the rabbit calvarium

Application of a Novel Resorbable Membrane in the Treatment of Calvarial Defects in Rats

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