Molding topologically-complex 3D polymer microstructures from femtosecond laser machined glass

Schaap, Allison; Bellouard, Yves

doi:10.1364/ome.3.001428

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…Corrective lenses were experimentally fabricated using TPL ( Figure 3-figure supplement 1a,b) and plastic molding replication 39 directly onto the glass coverslip (see Materials and methods).…”

Section: Fabrication Of Efov-microendoscopesmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

Antonini

Sattin

Moroni

et al. 2020

eLife

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Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (< 500 μm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-crosssection ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

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Section: Fabrication Of Efov-microendoscopesmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

Antonini

Sattin

Moroni

et al. 2020

eLife

View full text Add to dashboard Cite

show abstract

“…Corrective lenses were experimentally fabricated using TPL 33 ( Supplementary Fig. 1a,b) and plastic molding replication 37 directly onto the glass coverslip (see Methods). Fabricated lenses had profile largely overlapping with the simulated one (Fig.…”

Section: Fabrication Of Efov-microendoscopesmentioning

confidence: 99%

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice

Antonini

Sattin

Moroni

et al. 2020

Preprint

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Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤ 500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-crosssection ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

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“…A. Schaap et. al [9], use a two-step femtosecond laser direct-write technique and wet-etching process to fabricate monolithic glass micromolds with complex three-dimensional surface topologies, and demonstrate the replication of these structures in a soft polymer (polydimethylsiloxane, PDMS).…”

Section: Process Characterization and Some Application Of Femtosecondmentioning

confidence: 99%

Feature issue introduction: Progress in Ultrafast Laser Modifications of Materials

Canioni

Bellouard

Cheng

et al. 2013

Opt. Mater. Express

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Molding topologically-complex 3D polymer microstructures from femtosecond laser machined glass

Cited by 21 publications

References 31 publications

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness

Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice

Feature issue introduction: Progress in Ultrafast Laser Modifications of Materials

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