“Molecular Activity Painting”: schaltbare, lichtgesteuerte Manipulation in lebenden Zellen

Chen, Xi; Venkatachalapathy, Muthukumaran; Kamps, Dominic; Weigel, Simone; Kumar, Ravi; Orlich, Michael; Garrecht, Ruben; Hirtz, Michael; Niemeyer, Christof M.; Wu, Yao‐Wen; Dehmelt, Leif

doi:10.1002/ange.201611432

Angewandte Chemie

2017

DOI: 10.1002/ange.201611432

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“Molecular Activity Painting”: schaltbare, lichtgesteuerte Manipulation in lebenden Zellen

Xi Chen

Muthukumaran Venkatachalapathy

Dominic Kamps

et al.

Abstract: Die akute subzelluläre Rekrutierung von Proteinen ist eine leistungsfähige Methode zur Untersuchung biologischer Netzwerke. Hoch‐dynamische Signalprozesse an der Plasmamembran können bisher jedoch nur bedingt räumlich und zeitlich untersucht werden. Insbesondere durch laterale Diffusion an der Plasmamembran ist eine anhaltende lokale Steuerung molekularer Funktionen schwer zu realisieren. Hier wird die “Molekulare‐Aktivitäts‐Malerei” (Molecular Activity Painting, MAP) vorgestellt, eine neuartige Technik, die p… Show more

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Cited by 14 publications

References 33 publications

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Tunable and Photoswitchable Chemically Induced Dimerization for Chemo‐optogenetic Control of Protein and Organelle Positioning

Chen

2018

Angewandte Chemie

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The spatiotemporal dynamics of proteins and organelles play an important role in controlling diverse cellular processes. Optogenetic tools using photosensitive proteins and chemically induced dimerization (CID), which allow control of protein dimerization, have been used to elucidate the dynamics of biological systems and to dissect the complicated biological regulatory networks. However, the inherent limitations of current optogenetic and CID systems remain a significant challenge for the fine‐tuning of cellular activity at precise times and locations. Herein, we present a novel chemo‐optogenetic approach, photoswitchable chemically induced dimerization (psCID), for controlling cellular function by using blue light in a rapid and reversible manner. Moreover, psCID is tunable; that is, the dimerization and dedimerization degrees can be fine‐tuned by applying different doses of illumination. Using this approach, we control the localization of proteins and positioning of organelles in live cells with high spatial (μm) and temporal (ms) precision.

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Tunable and Photoswitchable Chemically Induced Dimerization for Chemo‐optogenetic Control of Protein and Organelle Positioning

Chen

2018

Angewandte Chemie

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Multidirectional Activity Control of Cellular Processes by a Versatile Chemo‐optogenetic Approach

et al. 2018

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The spatiotemporal dynamics of proteins or organelles plays avital role in controlling diverse cellular processes. However,a cute control of activity at distinct locations within ac ell is challenging.Aversatile multidirectional activity control (MAC) approach is presented, whichemploys aphotoactivatable system that may be dimerizedu pon chemical inducement. The system comprises second-generation SLF*-TMP (S*T) and photocaged NvocTMP-Cl dimerizers;where, SLF*-TMP features as ynthetic ligand of the FKBP(F36V) binding protein, Nvoc is ac aging group,a nd TMP is the antibiotic trimethoprim. Tw oMAC strategies are demonstrated to spatiotemporally control cellular signaling and intracellular cargo transport. The novel platform enables tunable,r eversible,and rapid control of activity at multiple compartments in living cells.

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DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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The evolution of DNA microarray technology has led to sophisticated DNA chips that are being used as routine tools for fundamental and applied genome research such as genotyping and expression profiling. Owing to their capability for highly parallel, site‐directed immobilization of complementary nucleic acids through canonical Watson–Crick base‐pairing, however, DNA‐modified surfaces can also be used for the assembly of complex surface architectures comprised of non‐nucleic acid compounds, such as proteins or colloidal materials. Furthermore, implementation of functional DNA devices and structural DNA nanotechnology can unlock the full potential of DNA surfaces. Based on case studies from diagnostics, sensing, proteome research, cell adhesion, and cell signaling, we show how classical gene sensors have developed into modern integrated systems and platforms for various applications in life sciences and materials research.

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“Molecular Activity Painting”: schaltbare, lichtgesteuerte Manipulation in lebenden Zellen

Cited by 14 publications

References 33 publications

Tunable and Photoswitchable Chemically Induced Dimerization for Chemo‐optogenetic Control of Protein and Organelle Positioning

Tunable and Photoswitchable Chemically Induced Dimerization for Chemo‐optogenetic Control of Protein and Organelle Positioning

Multidirectional Activity Control of Cellular Processes by a Versatile Chemo‐optogenetic Approach

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

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