Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounder<i>Paralichthys olivaceus</i>

Yang, Mingfang; Deng, Kailin; Pan, Mingzhu; Zhang, Yue; Sampath, Wijerath Wiriduge Hasitha Aruna; Zhang, Wenbing; Mai, Kangsen

doi:10.1111/anu.13013

Cited by 17 publications

(7 citation statements)

References 87 publications

(102 reference statements)

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“…There were no significant differences in the survival rate (94.22–95.56%) and feed efficiency ratio (1.38–1.42) of olive flounder among all the treatments after the feeding trial ( P < 0.05). The specific growth rate in the group of C16 (3.65) was significantly higher than those in the groups of C0 (3.57), C8 (3.42) and C24 (3.52) ( P < 0.05) (Supplementary Table S1 ) 33 – 35 .…”

Section: Resultsmentioning

confidence: 95%

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Liu

Deng

Pan

et al. 2020

Sci Rep

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The present study was conducted to estimate the effects of dietary carbohydrates on muscle quality and the underlying mechanisms. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of carbohydrates (0%, 8%, 12%, 16%, 20% and 24%, respectively). These diets were named as C0, C8, C12, C16, C20 and C24, respectively. After a 10-week feeding trial, results showed that the muscle pH, liquid holding capacity (LHC) and hardness were significantly decreased by the increasing dietary carbohydrate levels. Dietary carbohydrates significantly decreased the muscle fibre diameter, and the highest value was found in the C0 group. Accumulated glycogen and degenerated mitochondrial cristae were observed in the C24 group. Significantly higher contents of protein carbonyls were observed in the C20 group and C24 group (P < 0.05). There was a significant decrease of mtDNA copy number in the C24 group compared with that in the C0 and C8 groups. The AMP/ATP ratio in muscle decreased first and then increased with the increasing dietary carbohydrate levels. The dietary incorporation of carbohydrate significantly reduced the expression of opa1, pygm and genes involved in myogenesis (myf5 and myog). Meanwhile, proteolysis-related genes (murf-1, mafbx, capn2 and ctsl), pro-inflammatory cytokines (il-6 and tnf-α) and mstn were significantly up-regulated. In the C24 group, significant increase of phosphorylation of AMPK (Thr172), up-regulation of PGC-1α and GLUT4 were observed, while the phosphorylation level of S6 (Ser235/236) was significantly decreased. It was concluded that excessive dietary carbohydrate level (24%) had negative impacts on mitochondria function and promoted glycogen accumulation, and thereafter influenced the muscle quality of olive flounder. The activation of AMPK as well as the upregulation of PGC-1α and GLUT4 was the key mechanism.

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Section: Resultsmentioning

confidence: 95%

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Liu

Deng

Pan

et al. 2020

Sci Rep

Self Cite

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“…A high-carbohydrate diet might lead to a high level of plasma insulin, and further deposition of glycogen and lipids in liver and muscle. Meanwhile, it results in the dysregulation of glucose homeostasis [ 41 , 42 ]. However, carbohydrate metabolism is not the sole cause of dysglycemia; lipid metabolism is also one of the reasons [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Functions of Forkhead Box O on Glucose Metabolism in Abalone Haliotis discus hannai and Its Responses to High Levels of Dietary Lipid

Wang

Guo

Pan

et al. 2021

Genes

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The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the foxo homolog in abalone Haliotis discus hannai was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone foxo-specific siRNA (small interfering RNA) was injected to investigate the functions of foxo on glucose metabolism. Knockdown of foxo inhibited expression of phosphoenolpyruvate carboxykinase (pepck) and significantly increased expressions of hexokinase (hk) and pyruvate kinase (pk), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (g6pase). Then, a 100-day feeding trial was conducted to investigate the response of foxo and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis (hk) or improved gluconeogenesis significantly (pepck and g6pase). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone foxo can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role.

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“…GCK phosphorylates glucose to glucose-phosphate, which is the first step of glucose metabolism and occurs in the liver of animals [19], while HK catalyzes glucose phosphorylation at lower glucose levels [16]. Normally, GCK and HK expressions are positively related to dietary carbohydrate level [57]. Besides, upregulation of GCK is also beneficial for insulin secretion and promotes glucose uptake by the liver for glycogen synthesis to maintain the homeostasis of glucose [18].…”

Section: Aquaculture Nutritionmentioning

confidence: 99%

Transcriptomic Analysis and Histological Alteration of Black Sea Bream (Acanthopagrus schlegelii) Liver Fed Different Protein/Energy Ratio Diets

Wang

Sagada

Wang

et al. 2022

Aquaculture Nutrition

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To systematically investigate the effects of different protein/energy (P/E) ratio diets on the microstructure and metabolic response of black sea bream liver, fish (initial weight 11.20 ± 0.20 g) were fed three isoenergetic (20.9 kJ/g) diets with varying protein/lipid levels (LR, 28.8% protein/26.3% lipid; MR, 37.5% protein/20.9% lipid; and HR, 42.8% protein/17.3% lipid, P/E ratio increased gradually) for 8 weeks. The lipid and glycogen contents in the liver decreased with increasing dietary P/E ratios ( P < 0.05 ). Histological observation found that the liver of the LR group presented many adipose cells, along with polarized nucleus, massive lipid droplets, and unclear organelle structure. The liver cellular structures of MR and HR groups were normal with some small unmatured lipid droplets. RNA-Seq was applied to investigate the hepatic transcriptome response to different P/E ratio diets of black sea bream. Different levels of gene expressions were identified in the signaling pathways related to energy metabolism, amino acid metabolism, lipid metabolism, carbohydrate metabolism, immune system, etc. The LR diet inhibited the genes related to lipid oxidation, glycolysis, and protein metabolism, whereas the HR diet inhibited those of glycolysis and protein catabolism. The present study provides a comprehensive understanding of the molecular mechanisms behind the effects of different P/E ratio diets on the liver metabolism of black sea bream.

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Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

Cited by 17 publications

References 87 publications

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Functions of Forkhead Box O on Glucose Metabolism in Abalone Haliotis discus hannai and Its Responses to High Levels of Dietary Lipid

Transcriptomic Analysis and Histological Alteration of Black Sea Bream (Acanthopagrus schlegelii) Liver Fed Different Protein/Energy Ratio Diets

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