Molecular analysis and identification of the radC gene from the phototrophic bacterium Rhodobacter capsulatus B10

Katsiou, Eleni; Nickel, Christiane M.; García, Augusto F.; Tadros, Monier H.

doi:10.1016/s0944-5013(99)80020-2

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Nevertheless, the cause of this partial induction remains to be investigated. Interestingly, we also noticed that CtrA activates the expression of other genes related with stress responses such as gshB, radC, dnaE2, and yaaA (36)(37)(38)(39). Furthermore, RSWS8N_00955 that encodes a DNA photolyase showed a log 2 fold change (FC) of Ϫ1.95, just slightly below the selected threshold.…”

Section: Resultsmentioning

confidence: 89%

The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle

et al. 2020

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Activation of the two-component system formed by CckA, ChpT, and CtrA (kinase, phosphotransferase, and response regulator, respectively) in Rhodobacter sphaeroides does not occur under the growth conditions commonly used in the laboratory. However, it is possible to isolate a gain-of-function mutant in CckA that turns the system on. Using massive parallel transcriptome sequencing (RNA-seq), we identified 321 genes that are differentially regulated by CtrA. From these genes, 239 were positively controlled and 82 were negatively regulated. Genes encoding the Fla2 polar flagella and gas vesicle proteins are strongly activated by CtrA. Genes involved in stress responses as well as several transcriptional factors are also positively controlled, whereas the photosynthetic and CO2 fixation genes are repressed. Potential CtrA-binding sites were bioinformatically identified, leading to the proposal that at least 81 genes comprise the direct regulon. Based on our results, we ponder that the transcriptional response orchestrated by CtrA enables a lifestyle in which R. sphaeroides will effectively populate the surface layer of a water body enabled by gas vesicles and will remain responsive to chemotactic stimuli using the chemosensoring system that controls the Fla2 flagellum. Simultaneously, fine-tuning of photosynthesis and stress responses will reduce the damage caused by heat and high light intensity in this water stratum. In summary, in this bacterium CtrA has evolved to control physiological responses that allow its adaptation to a particular lifestyle instead of controlling the cell cycle as occurs in other species. IMPORTANCE Cell motility in Alphaproteobacteria is frequently controlled by the CckA, ChpT, and CtrA two-component system. Under the growth conditions commonly used in the laboratory, ctrA is transcriptionally inactive in Rhodobacter sphaeroides, and motility depends on the Fla1 flagellar system that was acquired by a horizontal transfer event. Likely, the incorporation of this flagellar system released CtrA from the strong selective pressure of being the main motility regulator, allowing this two-component system to specialize and respond to some specific conditions. Identifying the genes that are directly regulated by CtrA could help us understand the conditions in which the products of this regulon are required. Massive parallel transcriptome sequencing (RNA-seq) revealed that CtrA orchestrates an adaptive response that contributes to the colonization of a particular environmental niche.

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Section: Resultsmentioning

confidence: 89%

The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle

et al. 2020

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“…While not associated with the SOS regulon, its activity is enhanced in response to UV-induced DNA damage [71]. The function of RadC is still unclear, but it is thought to be involved in the repair of DNA strand breaks [71], [72]. We also noted that the RecN DNA repair protein (COG0497) is not encoded by the ‘ Ca.…”

Section: Resultsmentioning

confidence: 99%

The Complete Genome Sequence of ‘Candidatus Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra Chip Disease

et al. 2011

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Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This disease has led to multi-million dollar losses for growers in the central and western United States over the past decade and impacts the livelihood of potato farmers in Mexico and New Zealand. ZC is associated with ‘Candidatus Liberibacter solanacearum’, a fastidious alpha-proteobacterium that is transmitted by a phloem-feeding psyllid vector, Bactericera cockerelli Sulc. Research on this disease has been hampered by a lack of robust culture methods and paucity of genome sequence information for ‘Ca. L. solanacearum’. Here we present the sequence of the 1.26 Mbp metagenome of ‘Ca. L. solanacearum’, based on DNA isolated from potato psyllids. The coding inventory of the ‘Ca. L. solanacearum’ genome was analyzed and compared to related Rhizobiaceae to better understand ‘Ca. L. solanacearum’ physiology and identify potential targets to develop improved treatment strategies. This analysis revealed a number of unique transporters and pathways, all potentially contributing to ZC pathogenesis. Some of these factors may have been acquired through horizontal gene transfer. Taxonomically, ‘Ca. L. solanacearum’ is related to ‘Ca. L. asiaticus’, a suspected causative agent of citrus huanglongbing, yet many genome rearrangements and several gene gains/losses are evident when comparing these two Liberibacter. species. Relative to ‘Ca. L. asiaticus’, ‘Ca. L. solanacearum’ probably has reduced capacity for nucleic acid modification, increased amino acid and vitamin biosynthesis functionalities, and gained a high-affinity iron transport system characteristic of several pathogenic microbes.

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“…The expression of radC was reported to increase about fivefold after UV irradiation. However, the survival rate of radC mutant cells to mild UV treatment was not significantly different from that of the wild-type parent (10). In B. subtilis, radC belongs to the M -responsive genes (9).…”

Section: Figmentioning

confidence: 85%

“…In the facultative phototrophic bacterium Rhodobacter capsulatus, radC was shown to be relatively highly expressed under both chemotrophic and phototrophic growth conditions (10). The expression of radC was reported to increase about fivefold after UV irradiation.…”

Section: Figmentioning

confidence: 99%

RadC, a Misleading Name?

et al. 2008

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Molecular analysis and identification of the radC gene from the phototrophic bacterium Rhodobacter capsulatus B10

Cited by 7 publications

References 17 publications

The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle

The CtrA Regulon of Rhodobacter sphaeroides Favors Adaptation to a Particular Lifestyle

The Complete Genome Sequence of ‘Candidatus Liberibacter solanacearum’, the Bacterium Associated with Potato Zebra Chip Disease

RadC, a Misleading Name?

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