Molecular Analysis of Anthocyanin Biosynthetic Genes and Control of Flower Coloration by Flavonoid 3′,5′-Hydroxylase (F3′5′H) in Dendrobium moniliforme

Whang, Sung Soo; Um, Wan Sook; Song, In-Ja; Lim, Pyung Ok; Choi, Kyung; Park, Kwang-Woo; Kang, Kyung‐Won; Choi, Mi Sun; Koo, Jun Mo

doi:10.1007/s12374-011-9158-7

Cited by 31 publications

(21 citation statements)

References 44 publications

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“…Based on the PGSC database, PG0026477 and PG0000561 were annotated as StAN11, which was homologous to Arabidopsis TTG1. PG0026477b not only interacted with the MYB transcription factor and bHLH1 (PG0012891), but also directly interacted with multiple genes that were involved in the flavonoid biosynthesis pathway, such as ANS (PG0003090, PG0003091, PG0003880, PG0027333, PG0027396, and PG0033567), F3 5 H (PG0000425), DFR (PG0003605), F3H (PG0003563), and FLS (PG1024988) [76][77][78]. PG0026477, annotated as StAN11, plays the same role as the TTG1 gene in anthocyanin synthesis, which was reported to form a MBW complex with the MYB transcription factor and bHLH1.…”

Section: The Interaction Network Of Stan11 Proteinsmentioning

confidence: 99%

The WD40 Gene Family in Potato (Solanum Tuberosum L.): Genome-Wide Analysis and Identification of Anthocyanin and Drought-Related WD40s

Liu

Coulter

et al. 2020

Agronomy

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WD40 proteins, also known as WD40 domain proteins, constitute a large gene family in eukaryotes and play multiple roles in cellular processes. However, systematic identification and analysis of WD40 proteins have not yet been reported in potato (Solanum tuberosum L.). In the present study, 178 potato WD40 (StWD40) genes were identified and their distribution on chromosomes, gene structure, and conserved motifs were assessed. According to their structural and phylogenetic protein features, these 178 StWD40 genes were classified into 14 clusters and 10 subfamilies. Collinearity analysis showed that segmental duplication events played a major role in the expansion of the StWD40 gene family. Synteny analysis indicated that 45 and 23 pairs of StWD40 genes were orthologous to Arabidopsis and wheat (Triticum aestivum), respectively, and that these gene pairs evolved under strong purifying selection. RNA-seq data from different tissues and abiotic stresses revealed tissue-specific expression and abiotic stress-responsive StWD40 genes in doubled monoploid potato (DM). Furthermore, we further analyzed the WD40 genes might be involved in anthocyanin biosynthesis and drought stress in tetraploid potato cultivars based on RNA-seq data. In addition, a protein interaction network of two homologs of Arabidopsis TTG1, which is involved in anthocyanin biosynthesis, was constructed to identify proteins that might be related to anthocyanin biosynthesis. The result showed that there were 112 pairs of proteins interacting with TTG1, with 27 being differentially expressed in pigmented tissues. This study indicates that WD40 proteins in potato might be related to anthocyanin biosynthesis and abiotic stress responses.

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Section: The Interaction Network Of Stan11 Proteinsmentioning

confidence: 99%

The WD40 Gene Family in Potato (Solanum Tuberosum L.): Genome-Wide Analysis and Identification of Anthocyanin and Drought-Related WD40s

Liu

Coulter

et al. 2020

Agronomy

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“…Compared with cyanidin-based and pelargonidin-based anthocyanin, the formation of delphinine needed the expression of a special structural gene F3'5'H (Fig 1) [42]. The structural gene F3'5'H had a higher transcript level in blue aleurone than white, which could imply that delphinidin-based anthocyanin was responsible for the aleurone’s blue color in ‘Blue 1’.…”

Section: Discussionmentioning

confidence: 99%

ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum

Li¹,

Zhang

et al. 2017

PLoS ONE

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Blue aleurone is a useful and interesting trait in common wheat that was derived from related species. Here, transcriptomes of blue and white aleurone were compared for isolating Blue aleurone 1 (Ba1) transferred from Thinopyrum ponticum. In the genes involved in anthocyanin biosynthesis, only a basic helix-loop-helix (bHLH) transcription factor, ThMYC4E, had a higher transcript level in blue aleurone phenotype, and was homologous to the genes on chromosome 4 of Triticum aestivum. ThMYC4E carried the characteristic domains (bHLH-MYC_N, HLH and ACT-like) of a bHLH transcription factor, and clustered with genes regulating anthocyanin biosynthesis upon phylogenetic analysis. The over-expression of ThMYC4E regulated anthocyanin biosynthesis with the coexpression of the MYB transcription factor ZmC1 from maize. ThMYC4E existed in the genomes of the addition, substitution and near isogenic lines with the blue aleurone trait derived from Th. ponticum, and could not be detected in any germplasm of T. urartu, T. monococcum, T. turgidum, Aegilops tauschii or T. aestivum, with white aleurone. These results suggested that ThMYC4E was candidate Ba1 gene controlling the blue aleurone trait in T. aestivum genotypes carrying Th. ponticum introgression. The ThMYC4E isolation aids in better understanding the genetic mechanisms of the blue aleurone trait and in its more effective use during wheat breeding.

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“…Dendrobium moniliforme "Jindo" (Whang et al 2011) was cultivated under a 16-h light/8-h dark cycle at 24℃ in a greenhouse facility at Chonbuk National University. Leaf and root samples were harvested from young seedlings to isolate total RNA.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Promoter activity of constructs containing various lengths of DmACT1 5' flanking sequence were examined by a microprojectile bombardment-mediated transient assay (Whang et al 2011). Approximately 3.5 μg of gold particles (0.6 μm, Bio-Rad Laboratory, USA) were coated with 5 μg of DNA construct.…”

Section: Microprojectile Bombardmentmentioning

confidence: 99%

Isolation of an actin promoter for strong expression of transgenes in the orchid genus Dendrobium

Koo¹

2013

Journal of Plant Biotechnology

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We isolated and functionally characterized a Dendrobium Actin1 (DmACT1) promoter that drives strong gene expression in the orchid genus Dendrobium. A genomic fragment containing the region 3227 bp upstream of the coding region of DmACT1 was obtained by inverse PCR. Detailed comparison of the full-length cDNA and genomic sequences revealed that DmACT1 has a 1374 bp first intron in the 5' UTR. However, the 5' flanking sequences upstream of the coding region showed no obvious sequence similarities compared to those of known promoters, including plant actin promoters. Serial deletion constructs of the 5' flanking region from the translation initiation codon were fused to the coding sequence of a GUS/luciferase fusion reporter to identify the regulatory elements necessary for promoter activity. Transient assays in the flowers of Dendrobium revealed that the 5' UTR-intron greatly enhanced promoter activity. Moreover, the DmACT1 promoter with its 5' UTR-intron yielded approximately 10-fold higher reporter activity than the rice Act1 promoter-intron. Our data suggest that the DmACT1 promoter with its 5' UTR-intron is a useful tool for strong expression of transgenes in Dendrobium orchids.

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Molecular Analysis of Anthocyanin Biosynthetic Genes and Control of Flower Coloration by Flavonoid 3′,5′-Hydroxylase (F3′5′H) in Dendrobium moniliforme

Cited by 31 publications

References 44 publications

The WD40 Gene Family in Potato (Solanum Tuberosum L.): Genome-Wide Analysis and Identification of Anthocyanin and Drought-Related WD40s

The WD40 Gene Family in Potato (Solanum Tuberosum L.): Genome-Wide Analysis and Identification of Anthocyanin and Drought-Related WD40s

ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum

Isolation of an actin promoter for strong expression of transgenes in the orchid genus Dendrobium

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