Molecular analysis of ARF1 expression profiles during development of physic nut (Jatropha curcas L.)

Qin, Xiaobo; Lin, Fei; Lii, Yifan; Gou, Chunbao; Chen, Fang

doi:10.1007/s11033-010-0280-2

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…It is obvious that ARF1 is not developmentally regulated at the translational level, but is regularly expressed as a housekeeping gene, to a certain functional level during early developmental stages, in accordance with previous reports for plants (Kobayashi‐Uehara et al. 2001; Xu & Scheres 2005; Qin et al. 2011), but additional induced protein expression was observed in darkness.…”

Section: Discussionsupporting

confidence: 91%

Expression characteristics of ARF1 and SAR1 during development and the de‐etiolation process

et al. 2011

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ARF1 (ADP-ribosylation factor 1) and SAR1 (secretion-associated RAS super family) are involved in the formation and budding of vesicles throughout plant endomembrane systems. The molecular mechanisms of this transport have been studied extensively in mammalian and yeast cells. However, very little is known about the mechanisms of coat protein complex (COP) formation and recruitment of COP-vesicle cargoes in plants. To provide insights into vesicular trafficking in Pisum sativum L., we investigated mRNA and protein expression patterns of ARF1 and SAR1 in roots and shoots at early growth stages and in the de-etiolation process. We showed that ARF1 was concentrated mostly in the crude Golgi fractions, and SAR1 was concentrated predominantly in the crude ER fractions of de-etiolated shoots. ARF1 and SAR1 proteins were several times more abundant in shoots relative to roots. In total protein homogenates, the expression level of SAR1 and ARF1 was higher in shoots of dark-grown pea plants than light-grown plants. In contrast, ARF1 was higher in roots of light-grown pea relative to roots of dark-grown pea. With ageing, the ARF1 mRNA in roots was reduced, while SAR1 expression increased. Unlike ARF1 transcripts, ARF1 protein levels did not fluctuate significantly in root and shoot tissue during early development. The relative abundance of SAR1 protein in root tissues may suggest a high level of vesicular transport from the ER to the Golgi. Experimental results suggested that white light probably affects the regulation of ARF1 and SAR1 protein levels. On the other hand, short-term white light affects SAR1 but not ARF1.

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Section: Discussionsupporting

confidence: 91%

Expression characteristics of ARF1 and SAR1 during development and the de‐etiolation process

et al. 2011

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“…Similarly, ARF1 of Jatropha curcas is induced in response to abiotic stresses, particularly upon treatment with PEG, which induces osmotic stress, and cold treatment (Qin et al 2011 ).…”

Section: Arf Gtpasesmentioning

confidence: 99%

Small and Large G Proteins in Biotic and Abiotic Stress Responses in Plants

Pandey

Sharma

Pandey

2015

Elucidation of Abiotic Stress Signaling in Plants

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The sessile nature of plants has enabled them to develop a higher level of plasticity in adaptation against the hostile environmental conditions such as biotic and abiotic stresses. The intricate molecular machineries in plants, consisting of signal sensors, signal transducers, and generation of response, are highly similar with animal's signal transduction components; however, a large number of pathways also show a high level of divergence. G proteins are a part of signal transduction components, which play pivotal role in regulating a large number of physiological and developmental processes in both plants and animals. G proteins or guanine nucleotide-binding proteins serve as molecular switches, existing in two forms: an active conformation, when bound to GTP, and an inactive conformation, when bound to GDP. In active conformation, G proteins interact with downstream effector molecules to initiate various cellular responses. Based on their structure, they have been divided into heterotrimeric and monomeric G proteins, also called large and small G proteins, respectively, where the former is composed of three subunits (α, β, and γ) and latter is composed of single subunit (α). Plant growth and development is partially regulated by the combined activity of large and small G proteins. Besides, identifi cation of profuse number of stress-related genes interacting with monomeric and heterotrimeric G-protein complex in both Arabidopsis and rice suggests their involvement in modulation of abiotic stress responses. Further, based on the functional analysis of several G proteins (large and small) regulating cell growth, cell proliferation, seed germination, sugar sensing, and hormonal responses indicate these proteins are equally essential for plant development. Due to increased demand of food crops to sustain growing population in adverse environmental conditions, the role of G proteins and modulation of GTPase signaling has been studied in plants to produce genetically modifi ed crop plants with increased stress tolerance and productivity.

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